MORENO, CAROLINA S.ROGERO, SIZUE O.IKEDA, TAMIKO I.CRUZ, AUREA S.ROGERO, JOSE R.2014-11-172014-11-182015-04-022014-11-172014-11-182015-04-02MORENO, CAROLINA S.; ROGERO, SIZUE O.; IKEDA, TAMIKO I.; CRUZ, AUREA S.; ROGERO, JOSE R. In Vitro cell culture lethal dose submitted to gamma radiation. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 9th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 16th; MEETING ON NUCLEAR INDUSTRY, 1st, September 27 - October 2, 2009, Rio de Janeiro, RJ. <b>Proceedings...</b> Disponível em: http://repositorio.ipen.br/handle/123456789/16568.http://repositorio.ipen.br/handle/123456789/16568The present study was designed to evaluate the in vitro effect of gamma radiation in cell culture of mouse connective tissue exposed to different doses of gamma radiation and under several conditions. The cell viability was analyzed by neutral red uptake methodology. This assay was developed for establish a methodology to be used in the future in the study of resveratrol radioprotection. Resveratrol (3,4’,5- trihydroxystilbene), a phenolic phytoalexin that occurs naturally in some spermatophytes, such as grapevines, in response to injury as fungal infections and exposure to ultraviolet light. In the wines this compound is found at high levels and is considered one of the highest antioxidant constituents. The intense antioxidant potential of resveratrol provides many pharmacological activities including cardioprotection, chemoprevention and anti-tumor effects. Our results demonstrated that 60Co gamma radiation lethal dose (LD50) on NCTC clone 929 cells was about 340Gy.openAccessbiological radiation effectscell culturescobalt 60connective tissue cellsdose ratesdose-response relationshipsexperimental datagamma radiationin vitrolethal dosesradiation dosesradioprotective substancesratssurvival curvesTexto completo de eventohttps://orcid.org/0000-0002-2517-7772https://orcid.org/0000-0003-3396-6277