CAROLINE CRISTINA ELIAS
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Resumo IPEN-doc 26944 A new approach to obtain the catalytic sites region of human sACE with correct fold and activity2017 - AFFONSO, REGINA; SAMPAIO, SUELEN de B.; JANUARIO, FAGNER S.; PEREIRA, LARISSA M.; ARAGÃO, DANIELLE S.; CASARINI, DULCE E.; ELIAS, CAROLINE C.Angiotensin-converting enzyme I (ACE) is a membrane-bound that catalyzes the conversion of angiotensin I to the potent vasopressor angiotensin II. ACE is a key part of the renin-angiotensin system, which regulates blood pressure and is widely distributed throughout the body. There are two isoforms of human ACE, including the somatic ACE (sACE) present in somatic tissue and the testicular ACE (tACE) present in male germinal cells. The sACE possesses two domains, N- C- domains, with catalytic sites which exhibit 60% sequence identity. These domains differ in terms of chloride-ion activation profiles, rates of peptide hydrolysis of angiotensin I, bradykinin, Goralatide, Luliberin, substance P, angiotensina, beta-amyloid peptide and sensitivities to various inhibitors. A more detailed analysis shows that these regions are composed of HEMGH and EAIGD sequences that bind zinc ions to facilitate catalytic activity (Fig. 1). Our question is: If the synthesis of catalytic sites with corrects structure and activity could be a good model per si to study new drugs. The objective was to obtain the Ala361 a Gli468 and Ala959 to Ser1066 catalytic regions sACE in a structural conformation that resembles its native form. The catalytic regions were obtained from bacterial system; the expression of this protein in soluble form enables completion of the solubilization/purification steps without the need for refolding. The characterization of Ala959 to Ser1066 region shows that this has an α-helix and β-strand structure, Fig. 1b, which zinc ion (essential for its activity) binds to, and with enzymatic activity. Our conclusion is that the strategy used to obtain the Ala959 to Ser1066 region in the correct structural conformation and with activity was successful.