CARLOS HENRIQUE DE MESQUITA

CARLOS HENRIQUE DE MESQUITA

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Increased adiposity by feeding growing rats a high-fat diet results in iron decompartmentalisation
2020 - LOBO, ALEXANDRE R.; GAIEVSKI, EDUARDO H.S.; MESQUITA, CARLOS H. de; DE CARLI, EDUARDO; TEIXEIRA, PRYSCILA D.S.; PEREIRA, ROSA M.R.; BORELLI, PRIMAVERA; SÁ, LILIAN R.M. de; COLLI, CELIA
The present study reports the effects of a high-fat (HF) diet of over 8 weeks on the Fe status of growing rats. Tissue Fe levels were analysed by atomic absorption spectrophotometry, and whole-body adiposity was measured by dual-energy X-ray absorptiometry. Histopathology and morphometry of adipose tissue were performed. Liver homogenates were used for measuring ferroportin-1 protein levels by immunoblotting, and transcript levels were used for Fe genes measured by real-time PCR. Tissue Fe pools were fit to a compartmental biokinetic model in which Fe was assessed using fourteen compartments and twenty-seven transfer constants (kj,i from tissue ‘i’ to tissue ‘j’) adapted from the International Commission on Radiological Protection (ICRP) 69. Ten kj,i were calculated from the experimental data using non-linear regression, and seventeen were estimated by allometry according to the formula ki;j ¼ a ×Mb. Validation of the model was carried out by comparing predicted and analysed Fe pool sizes in erythrocytes, the liver and the spleen. Body adiposity was negatively associated with serum Fe levels and positively associated with liver Fe stores. An inferred increase in Fe transfer from bone marrow to the liver paralleled higher hepatic Fe concentrations and ferritin heavy-chain mRNA levels in the HF diet-fed animals, suggesting that liver Fe accumulation occurred at least in part due to a favoured liver erythrocyte uptake. If this feeding condition was to be prolonged, impaired Fe decompartmentalisation may occur, ultimately resulting in dysmetabolic Fe overload.
Plasma clearance of chylomicron remnants is delayed in aged subjects
2018 - TAVONI, T.M.; VINAGRE, C.G.; FREITAS, F.R.; MESQUITA, C.H.; VINAGRE, J.C.; MARIANI, A.C.; KALIL-FILHO, R.; MARANHAO, R.C.
Aim: Dietary fats absorbed in the intestine are transported in the circulation as chylomicrons and remnants that have atherogenic potential. Although postprandial lipidemia is increased in older subjects, the specific chylomicron metabolism has not been explored in older subjects nor compared to young subjects, which is the focus of this study. Methods: After a 12 h fast, artificially-made emulsions similar to lymph chylomicrons and doubly labeled with radioactive cholesteryl esters and triglycerides were intravenously injected in 23 older (66±4 years) and 20 young (24±3 years) subjects. Sequential blood samples were collected to determine fractional clearance rates (FCR, in min-1) by compartmental analysis. Results: Older subjects had higher LDL-cholesterol (p<0.001) and triglycerides (p<0.0001) than young subjects; HDL-cholesterol presented no difference. The emulsion cholesteryl-ester FCR was lower in older subjects compared to the young (p¼0.0001). The emulsion triglyceride FCR did not differ in the two groups. Tested in vitro, however, the lipolysis of the emulsion triglycerides was less intense in the older than in the young subjects. Conclusions: As delayed removal of remnants, indicated by the pronouncedly smaller cholesteryl ester FCR, is related to the presence of cardiovascular diseases, this can be a risk factor which could accelerate atherogenic complications occurring in aged subjects.
Removal of chylomicron remnants from the bloodstream is delayed in aged subjects
2018 - VINAGRE, CARMEN G.; FREITAS, FATIMA R.; MESQUITA, CARLOS H. de; VINAGRE, JULIANA C.; MARIANI, ANA C.; KALIL-FILHO, ROBERTO; MARANHAO, RAUL C.
Dietary fats absorbed in the intestine are transported in the circulation as chylomicrons and remnants that have atherogenic potential. Although postprandial lipidemia is increased in older subjects, the specific chylomicron metabolism has not been explored in older subjects nor compared to young subjects, which is the focus of this study. After a 12 h fast, artificially-made emulsions similar to lymph chylomicrons and doubly labeled with radioactive cholesteryl esters and triglycerides were intravenously injected in 23 older (66±4 years) and 20 young (24±3 years) subjects. Sequential blood samples were collected to determine fractional clearance rates (FCR, in min-1) by compartmental analysis. Older subjects had higher LDL-cholesterol (p<0.001) and triglycerides (p<0.0001) than young subjects; HDL-cholesterol presented no difference. The emulsion cholesteryl-ester FCR was lower in older subjects compared to the young (p=0.0001). The emulsion triglyceride FCR did not differ in the two groups. Tested in vitro, however, the lipolysis of the emulsion triglycerides was less intense in the older than in the young subjects. As delayed removal of remnants, indicated by the pronouncedly smaller cholesteryl ester FCR, is related to the presence of cardiovascular diseases, this can be a risk factor which could accelerate atherogenic complications occurring in aged subjects
Favorable effects of ezetimibe alone or in association with simvastatin on the removal from plasma of chylomicrons in coronary heart disease subjects
2014 - MANGILI, OTAVIO C.; GAGLIARDI, ANA C.M.; MANGILI, LEONARDO C.; MESQUITA, CARLOS H.; CESAR, LUIZ A.M.; TANAKA, AKIRA; SCHAEFER, ERNST J.; MARANHAO, RAUL C.; SANTOS, RAUL D.
Objective: Reductions on the clearance from plasma of chylomicrons are associated with atherosclerosis. Statins improve the removal from plasma of chylomicrons in a dose dependent manner. There is controversy whether ezetimibe modifies the plasma clearance of chylomicrons. Effects of ezetimibe alone or in combination with simvastatin were compared with low and high dose of the latter, upon the kinetics of a chylomicron-like emulsion in coronary heart disease (CHD) patients. Methods: 25 CHD patients were randomized for treatment with ezetimibe 10 mg (group 1) or simvastatin 20 mg (group 2) with progression to ezetimibe þ simvastatin 10/20 mg or simvastatin 80 mg, respectively. Kinetic studies were performed at baseline and after each treatment period of 6 weeks. The fractional catabolic rates (FCR) of the emulsion labeled with 14C-CE and 3 H-TG, that represent respectively chylomicron remnant and triglyceride removal, were calculated. Comparisons were made by ANOVA. Results: The 14CE-FCR in group 1 were 0.005 0.004, 0.011 0.008 and 0.018 0.005 min1 and in group 2 were 0.004 0.003, 0.011 0.008 and 0.019 0.007 min1 respectively at baseline, after 6 and 12 weeks (p < 0.05 vs. baseline, and 6 vs. 12 weeks). The 3 H-TG-FCR in group 1 were 0.017 0.011, 0.024 0.011 and 0.042 0.013 min1 and in group 2 were 0.016 0.009, 0.022 0.009 and 0.037 0.012 min1 at baseline, after 6 and 12 weeks (p < 0.05 vs. baseline, and 6 vs. 12 weeks). There were no differences between groups in time. Conclusion: Both treatments increased similarly the removal from plasma of chylomicron and remnants in CHD patients.
High cholesterol intake modifies chylomicron metabolism in normolipidemic young men
2006 - CESAR, THAIS B.; OLIVEIRA, MARIA R.M.; MESQUITA, CARLOS H.; MARANHAO, RAUL C.
The rise of the plasma lipid concentration elicited by dietary sodium chloride restriction in Wistar rats is due to an impairment of the plasma triacylglycerol removal rate.)
2001 - CATANOZI, S.; ROCHA, J.C.; NAKANDAKARE, E.R.; PASSARELLI, M.; MESQUITA, C.H.; SILVA, A.A.; DOLNIKOFF, M.S.; HARADA, L.M.; QUINTAO, E.C.R.; HEIMANN, J.C.