AMANDA CARAMEL JUVINO

AMANDA CARAMEL JUVINO

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Caries preventive action of Nd:YAG and fluoride in three different pH conditions
2023 - JUVINO, AMANDA C.; ZANINI, NATHALIA A.; AVELINO, SABRINA G.; OLIVEIRA, YASMIN R.F. de; GERMANO, GLEICE C.M.; EDUARDO, CARLOS de P.; ZEZELL, DENISE M.
This in vitro study aimed to evaluate the preventive action of topical fluoride application combined with laser irradiation under different pH conditions using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning ElectronMicroscopy (SEM). A total of 180 samples of human dental enamel were prepared and divided into groups: Negative Control, Fluoride (FFA 12.300 F􀀀/g), Laser (Nd:YAG 84.9 J/cm2), and Laser + Fluoride (Nd:YAG 84.9 J/cm2 + FFA 12.300 F􀀀/g). The pH cycling was performed at three different pH conditions: pH 5 (below the critical pH for hydroxyapatite), pH 4.5 (below the critical pH in the presence of fluorapatite), and pH 4 (investigating acid resistance of hydroxyapatite and fluorapatite forms with laser irradiation). In the FTIR analysis, the Laser + Fluoride group demonstrated statistically significant differences compared to the Negative Control group and Fluoride group at pH 4.5 and pH 4 when evaluating the phosphate bands. Similar results were observed in the SEM analysis, where the Laser + Fluoride group exhibited lower demineralization compared to the other treatments at pH 4.5 and pH 4. In conclusion, the Laser + Fluoride group demonstrated a significant reduction in demineralization even at pH levels below the critical threshold for fluorapatite, highlighting its superior acid resistance compared to fluoride alone.
Identification of enamel demineralization using high performance convolutional neural network
2022 - CARAMEL-JUVINO, AMANDA; FAROOQ, SAJID; ROMANO, MARIANA; ZEZELL, DENISE M.
Here, we traces use segmentation and convolutional neural network (CNN) to trace, diagnose and quantify enamel demineralization for research. The preprocessing, histograms based methods are used to enhance the contrast and equalize the brightness through the scanning electron microscope images. Our result evidence that the deep learning based CNN model is highly efficient to process the dental image to achieve high accuracy of enamel demineralization and presents promising outcomes with optimal precision.
Evaluation of the anti-caries effect beyond the critical enamel pH of preventive treatment of fluoride associated with Nd:YAG laser irradiation
2021 - JUVINO, AMANDA C.; ROMANO, MARIANA; ALVES, NATHALIA Z.; FREITAS, THAIS R.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
This study aimed to evaluate the anti-caries effect of fluoride associated with Nd:YAG laser irradiation in the h-eatment of enamel Eight groups (n = S) were analyzed: Negative Control pH 4.5; Negative control pH 4; Fluoride pH 4.5; Fluoride pH 4; Nd-YAG pH 4.5; Nd-YAG pH 4; (Fluoride + Nd-YAG) pH 4.5 and (Fluoride + Nd-YAG) pH 4. All samples were analyzed by Scanning Electron Microscopy (SEM) before and after the cycle. Quantification of phosphorus in the cycling solutions was carried out using the colorimetric method, as an indication of enamel demineralization. The anti-caries effect of the Fluoride + Nd: YAG treatment in addition to the critical pH of the enamel can be observed in the morphological analysis, however in the analysis of the demineralization solutions, the amount of phosphorus showed a difference only in the Fluoiide group compared to the others in the investigative pH.
Morphological, optical, and elemental analysis of dental enamel after debonding laminate veneer with Er,Cr:YSGG laser
2021 - ZANINI, NATHALIA A.; RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; CARAMEL-JUVINO, AMANDA; ANA, PATRICIA A.; ZEZELL, DENISE M.
Laminate veneer removal is becoming a routine procedure at the dental clinic and the use of laser can facilitate its removal. This work aimed to evaluate the morphological, elemental, and optical changes in the remaining enamel after veneer removal using Er,Cr:YSGG laser. Forty‐four enamel slabs were prepared and randomly distributed into nine experimental groups, for bonding using lithium disilicate laminates with three different luting agents (Variolink Veneer, RelyX U200, and RelyX Veneer). Then each agent was debonded using Er,Cr:YSGG laser (2.78 μm) using two different protocols:3.5 W, 48.14 J/cm2, 20 Hz non‐contact and 3.0 W, 48.14 J/cm2, 20 Hz non‐contact. The morphological, optical, and elemental analysis of enamel was performed before cementation and after laser debonding, using scanning electron microscopy (SEM), optical coherence tomography (OCT), and energy‐dispersive X‐ray spectroscopy (EDS). The level of statistical significance adopted was 5%. The EDS analysis of enamel after debonding revealed a significant increase in silane and carbon, as well as a decrease in calcium and phosphate contents. Analysis showed the presence of residual cement in most experimental groups but the morphological analysis showed alteration of the enamel's prisms only in the groups that used RelyX Veneer and Variolink Veneer cements. There was no evidence of deleterious morphological changes resulting from irradiation. However, an increase in the optical attenuation coefficient by the OCT was observed due to the presence of the remaining cement. It can be concluded that the Er,Cr:YSGG laser, in the mean powers used, is efficient for veneer removal without causing deleterious effects for the enamel.
Human dental enamel evaluation after radiotherapy simulation and laminates debonding with Er,Cr:YSGG using SEM and EDS
2019 - RABELO, THAIS F.; ZAMATARO, CLAUDIA B.; KUCHAR, NIELSEN G.; ZANINI, NATHALIA; JUVINO, AMANDA C.; DEL-VALLE, MATHEUS; CASTRO, PEDRO A.A.; SANTOS, MOISES O.; ZEZELL, DENISE M.
The pursuit of perfection makes younger people undergo aesthetic procedures without formal indication. However, young patients may be susceptible to a disease such as head and neck cancer which treatment can compromise the adhesion of these indirect mate-rials. Here, we present an analyze, of the gamma radiation effects on crystallographic morphology of human dental enamel after laminate veneer debonding with Er,Cr:YSGG laser. Thus, human dental enamel samples were prepared and randomized into 2 groups (n=10): Laser Irradiation (L) and Gamma + Laser Irradiation (GL) group. Scanning elec-tron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were performed before bonding and after debonding using Er,Cr:YSGG. Only Gamma + Laser Irradia-tion group received a cumulative dose of 70 Gy gamma radiation used in head and neck cancer radiotherapy. SEM images showed that both GL and L groups presented altered morphology. EDS showed an decrease in Ca and P intensities after laser debonding of laminates veneers in both group. Therefore, a proper laser facet removal protocol should be established for healthy patients and patients who have been exposed to radiotherapy for head and neck cancer.