NATHALIA FONSECA BOIANI

NATHALIA FONSECA BOIANI

Página do item completo

Resultados de Busca

Agora exibindo 1 - 8 de 8

Electron beam irradiation applied for the detoxification and degradation of single ciprofloxacin aqueous solution and multiclass pharmaceutical quaternary mixture
2023 - TOMINAGA, FLAVIO K.; BOIANI, NATHALIA F.; SILVA, THALITA T.; SANTOS, JONAS G. dos; LEBRE, DANIEL T.; LEO, PATRICIA; BORRELY, SUELI I.
The application of electron beam irradiation for detoxification and degradation of single antibiotic ciprofloxacin (CPF) and in a mixture with multiclass pharmaceuticals in aqueous solutions was carried out. Ecotoxicity assays indicated that the green algae were most sensitive to antibiotic and also that the presence of several pharmaceutical increased the toxicity. After the irradiation treatment, degradation results of single antibiotic indicated reduction of 95.86 % at 1.0 kGy. Total organic carbon decreased up to 38 % at 5.0 kGy. At lower doses (1.0 kGy), no effect in toxicity was evidenced, however, increase in toxicity for Vibrio fischeri was observed after 2.5 kGy. For Daphnia similis exposure, an increase in toxicity was noted for all applied doses. In contrast, for the green algae R. subcapitata toxicity reduction varied from 62.3 to 81.9 % at the evaluated doses. Toxicity assays to microbes E. coli and S. aureus reduced antibacterial activity of CPF after irradiation treatment. Regarding the irradiated quaternary mixture at 2.5 kGy, reduction up to 96 % was achieved for the ciprofloxacin, metformin and acetylsalicylic acid, and 81 % removal was achieved for fluoxetine. Acute assays with V. fischeri indicated no increase in toxicity, while some increase was noted for D. similis (acute effects). Nevertheless, chronic assays data indicated low toxicity reduction (14 %) with D. similis, and complete detoxification was shown for the green algae after the irradiation. In addition, decrease in antimicrobial activity was noted after the treatment. Furthermore, the in-silico model was not enough accurate for the prediction of CIP toxicity. These findings showed that electron beam irradiation can be applied for reducing the impacts of antibiotics in aquatic ecosystem. Measuring toxicity on living-organism from different trophic levels are useful tools to evaluate the interaction of mixtures and also to assess toxicity of the generated byproducts.
Application of electron beam irradiation for remediation of pharmaceutical compounds in water
2022 - TOMINAGA, F.K.; BOIANI, N.F.; SILVA, T.T.; LEO, P.; BORRELY, S.I.
A significant number of pharmaceutical active compounds have been released in the aquatic environment. These compounds are not fully removed from water and wastewater treatment plants. Furthermore, these contaminants are not commonly monitored, and they possess the potential to cause adverse ecological and human health effects. Electron Beam Irradiation (EBI) have been applied as an alternatively green method in water management, being efficient for removing organic recalcitrant pollutants at low doses. This work aims to assess the effect of EBI on toxicity of four pharmaceuticals from distinct class (anti-inflammatory, antidepressant, antibiotic, and antidiabetic) using organism from different trophic levels. Acetylsalicylic acid and fluoxetine hydrochloride were obtained from Labsynth (>99.5%) and Divis Pharmaceuticals Pvt. Ltd (98.8%), respectively. Metformin hydrochloride (97%) and ciprofloxacin (>98%) were purchased from Sigma- Aldrich. All aqueous solution were diluted using ultra-pure water. Acute toxicity assays with Daphnia similis and Vibrio fischeri were based on ABNT/NBR standard methods. The evaluated endpoint was immobility and bioluminescence inhibition, respectively. The yeast assays were carried by monitoring of changes in the specific conductivity of suspensions of S. cerevisiae. All the assays were performed in triplicate. The toxicity results of the microcrustacean and the bacteria were expressed in Toxicity Factor. For the yeast, data were analyzed by F-test and t-test using a significance level of 0.05. The UV-Vis spectrum showed changes in all pharmaceutical’s spectrum after irradiation at 2.5 kGy. The toxicity results indicated that the effects varied depending on the organism and the studied pharmaceutical. For acetylsalicylic acid, increase of toxicity was observed for all three the organism. In contrast, for fluoxetine, a great toxicity removal was achieved for D. similis while and for the bacteria and the yeast no changes of toxicity were noted. Regarding metformin, the radiolytic byproducts were only toxic to the microcrustacean, possibly including residual hydrogen peroxide. Finally, for ciprofloxacin, toxicity increase was verified for D. similis and V. fischeri, while detoxification was observed for S. cerevisiae. In conclusion, the present work demonstrated EBI is effective for removing pharmaceuticals and showed the importance of using different organism for toxicity assessment.
Assessment of binary mixture toxicity of pharmaceuticals of environmental concern to aquatic organisms
2022 - BOIANI, N.F.; TOMINAGA, F.K.; SILVA, T.T.; REDÍGOLO, M.M.; BORRELY, S.I.
Pharmaceutical products have been frequently detected in aquatic environments as mixtures. They may cause direct toxic effects to aquatic organisms and indirect effects on ecosystems, due to interactions which induce additive, synergistic or antagonistic effects. The classical mathematical models of concentration addition and independent action of pollutants have been extensively used for predicting the mixture effects of chemicals of environmental relevance. The objective of this study was to assess the binary mixture toxicity of pharmaceuticals: fluoxetine and propranolol; fluoxetine and sulfadiazine; fluoxetine and caffeine, in acute tests with Daphnia similis carried out based on ABNT/NBR standard methods. The prediction of the acute effects to binary mixture was performed considering the mentioned mathematical models and deviations of them (synergism/antagonism; concentration level-dependent or concentration ratio-dependent), using an automated Excel spreadsheet. For the mixture of propranolol + fluoxetine, the concentration addition model best described the mixture effects, which explained most of the variability of acute toxic responses. Through the concentration level-dependent, synergism at low doses and antagonism at high doses was pointed out. The concentration addition model better described the effects of the binary mixture of sulfadiazine + fluoxetine. Synergism related to the independent action model and an antagonism related to the concentration ratio-depend were identified. For caffeine + fluoxetine mixture, the concentration addition model described slightly better the effects of this binary mixture, synergism concentration level-dependent and dose ratio-dependent were identified. We can conclude that the nature of the interaction between the compounds of a mixture depends on effect level and on the ratio in which each one is applied. Major effects of the binary mixture were detected in the zone between the effects predicted by the evaluated models. The synergism/antagonism or additivity do not depend on the similarity/dissimilarity of the mode of action of the compounds of a mixture, showing a different behaviour respect to the theoretical assumptions.
Toxicidade aguda e avaliação de riscos ecológicos de fármacos pertencentes a diferentes classes detectados em ambientes aquáticos
2021 - TOMINAGA, F.K.; BOIANI, N.F.; GARCIA, V.S.G.; SILVA, T.T.; LEO, P.; BORRELY, S.I.
Avaliação da toxicidade da mistura de fármacos submetidos a irradiação por feixe de elétrons
2021 - BOIANI, N.F.; SILVA, T.T.; TOMINAGA, F.K.; GARCIA, V.S.G.; BORRELY, S.I.
Acute and chronic ecotoxicological effects of pharmaceuticals and their mixtures in Daphnia similis
2022 - TOMINAGA, FLAVIO K.; BOIANI, NATHALIA F.; SILVA, THALITA T.; GARCIA, VANESSA S.G.; BORRELY, SUELI I.
Pharmaceuticals have increasingly received attention from the scientific community due to their growing intake, improved detection and potential ecological risks. Several pharmaceuticals, including antidepressants, anti-inflammatory and antidiabetic compounds and antibiotics, have been described as contaminants in different water matrices. In this context, the aim of the present study was to assess the acute and chronic effects of four classes of pharmaceuticals (acetylsalicylic acid, fluoxetine, metformin and ciprofloxacin) individually and in binary and quartenary mixture. Furthermore, the toxicity of binary mixtures containing the antidepressant fluoxetine was also evaluated. The results of the single acute and chronic toxicity assays indicate lower acetylsalicylic acid and higher fluoxetine toxicity towards Daphnia similis. Regarding the evaluated mixture toxicity, the nature of potential toxicological interactions was predicted by applying mathematical concentration addition and independent action models. The findings revealed both antagonistic and synergistic features, depending on the applied amounts and doses. Finally, the chronic assays performed with the quaternary mixture indicated the presence of a hormetic effect at low concentrations. In sum, the present study demonstrated that the effects of individual pharmaceuticals can underestimate the risk level of these contaminants in the environment.
Avaliação da toxicidade de surfactantes não iônico e aniônico submetidos a tratamento por feixe de elétrons
2021 - GARCIA, VANESSA S.G.; TOMINAGA, FLAVIO K.; BOIANI, NATHALIA F.; SILVA, THALITA T.; ROSA, JORGE M.; BORRELY, SUELI I.
Is ionizing radiation effective in removing pharmaceuticals from wastewater?
2021 - TOMINAGA, FLAVIO K.; SILVA, THALITA T.; BOIANI, NATHALIA F.; JESUS, JULIANA M.S. de; TEIXEIRA, ANTONIO C.S.C.; BORRELY, SUELI I.
Wastewater and effluent discharges are the main causes of receiving water body pollution and important challenges in water quality management. Among the emerging contaminants, pharmaceuticals have increasingly drawn attention due to their incomplete removal during conventional biological treatment, inducing potential and actual risks to living organisms following residue discharges in river effluent. Electron beam irradiation (EBI) is a clean process technology for organic compound degradation and mineralization, as well as persistent pollutant detoxification. This study aimed to evaluate EBI effects on the degradation and toxicity removal of anti-inflammatory aspirin (ASA) in a single solution and in a fluoxetine (FLX) mixture. Results indicate that 98% of the single aspirin was degraded at 5.0 kGy. Aspirin toxicity to Daphnia similis, however, increased with increasing absorbed dose (1.0 to 5.0 kGy), possibly as a result of the presence of H2O2 and other byproducts formed during the oxidation process. Regarding the irradiated mixture, complete degradation was achieved for both pharmaceuticals. Toxicity removals for the mixture were of 56.2 ± 0.9% and 58.8 ± 5.4% for 1.0 and 2.5 kGy, respectively. These findings demonstrate that EBI can be an interesting alternative process to be applied as a pre-treatment followed by biological treatment.