RODRIGO FERNANDO BRAMBILLA DE SOUZA
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Artigo IPEN-doc 29025 Application of egg yolk IgY on carboxylated polypyrrole films for impedimetric detection of PfHRP2 antigen2022 - GANDARILLA, ARIAMNA M.D.; GLORIA, JULIANE C.; BARCELAY, YONNY R.; SOUZA, RODRIGO F.B. de; MARIUBA, LUIS A.M.; BRITO, WALTER R.This paper described an impedimetric immunosensor for detecting Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Antibodies from egg yolk (Ab-PfHRP2, IgY type) were linked covalently to the screen-printed gold electrodes (SPGE) surface modified with a thin film of Poly-pyrrole-pyrrole 3 carboxylic acid (P(Py-Py3COOH) to develop the sensing platform. The fabrication steps were followed by microscopic (scanning electron microscopy), spectroscopic (RAMAN spectroscopy and Energy-dispersive X-ray spectroscopy), and electrochemical (electrochemical impedance spectroscopy (EIS) and cyclic voltammetry) techniques. The determination of Ag-PfHRP2 was performed by EIS, and the BSA(bovine serum albumin)/Ab-PfHRP2(IgY)/P(Py-Py3COOH)/SPGE immunosensor recorded a linear response at 100–1000 ng mL−1 concentration range, with a limit of detection (LOD) of 27.47 ng mL−1. Its performance was confirmed by Enzyme-Linked Immuno-Sorbent Assay. The fabricated device uses a simple strategy of IgY immobilization, showing high sensitivity and good selectivity, and can be considered an alternative for carrying out malaria tests.Artigo IPEN-doc 25790 Microbial fuel cell‑induced production of fungal laccase to degrade the anthraquinone dye Remazol Brilliant Blue R2019 - SIMÕES, MARTA F.; MAIORANO, ALFREDO E.; SANTOS, JONAS G. dos; PEIXOTO, LUCIANA; SOUZA, RODRIGO F.B. de; OLIVEIRA NETO, ALMIR; BRITO, ANTONIO G.; OTTONI, CRISTIANE A.The anthraquinone dye Remazol Brilliant Blue R is largely used in the textile industry. However, its removal from wastewaters is costly and complex. Many methods have been tested to solve this ecological problem, but there is still a need for efficient methods. We propose here an alternative use of a two-chambered microbial fuel cell (MFC), fuelled with domestic wastewater in the anodic chamber, to degrade a simulated textile dye effluent made of Remazol Brilliant Blue R inoculated with an immobilised fungal strain, Pleurotus ostreatus URM 4809, as a laccase producer, in the cathodic chamber. The MFC showed continuous synthesis of laccase in the cathodic chamber, which, in turn, promoted the rapid decolourisation, of more than 86% of the textile dye effluent. The yield was further increased by the addition of glycerol. Electrochemical monitoring also indicated an increase in power density and current density. After 20 days of MFC operation, 62.1% of organic matter was removed in the anodic compartment, thus leaving the effluent with a much lower toxicity.