CLAUDIA BIANCHI ZAMATARO

CLAUDIA BIANCHI ZAMATARO

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In vivo study of diode laser 940 nm operating in high intensity mode in the periimplantitis disease
2018 - GOTTARDI, MARIELLA da S.; KAWAMOTO, DIONE; ZAMATARO, CLAUDIA B.; ANA, PATRICIA A. da; MAYER, MARCIA P.A.; ZEZELL, DENISE M.
The evolution of periimplantitis has been associated with several risk factors. The standard treatment consists on subgingival scaling and blasting with sodium bicarbonate. This 6 months longitudinal clinical study evaluated and compared two treatment methods: conventional as described and complemented by irradiation with high intensity diode laser (940 nm, Biolase) aiming to establish a more efficient periimplantitis management. After approval by the Human Research Ethics Committee, 20 patients of both sexes, with implants in function for at least 12 months and with diagnosis of periimplantitis were selected and randomly distributed in two experimental groups. Ten of them were conventionally treated as control group, and the remaining ten were in the laser treated group (1W, 4.24kJ/ cm2 energy density, pulsed mode, 30s, twice). There were six sites for treatment standardization: vestibular region (mesial, central and distal thirds) and buccal region (mesial, central and distal thirds). Results has shown that overall clinical evaluation has no significant difference between groups control and treatment. less bleeding and suppuration, as shallower periodontal pocket indicated a decreasing tendency on laser treated group. Quantitation of total bacteria and Porphyromona gingivalis by real-time PCR shown that laser synergy is a complementary therapy that could act as an additional option to the conventional treatment in difficult response cases.
Effect of Nd:YAG laser on caries prevention of dental enamel by optical attenuation coefficient with optical coherence tomography (OCT)
2018 - MORAES, MARCIA C.D.; PEREIRA, DAISA L.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.; FREITAS, ANDERSON Z.
Caries is a multifactorial disease, caused by the continuing loss of hydroxyapatite crystals. The association of topical acidulated phosphate fluoride application (APF) and high intensity lasers has the potencial to increase the resistance to demineralization of dental tissues. The Nd:YAG laser, specially associated to a photoabsorber over the dental surface, may induce thermal changes in order to increase the resistance to demineralization. Optical Coherence Tomography (OCT) is a non-ionizing radiation imaging technique that can be performed in real time in vivo and can detect early changes in the optical properties of t issues. This study aimed to characterize the effectiveness of the association of Nd:YAG la ser and APF gel in the prevention of caries lesions in human dental enamel in vitro, due to structural changes in function of the pH cycling, the treatments performed as well as to evaluate the potential of OCT to diagnose early demineralization. 160 human enamel samples were divided in four groups and submitted to pH cycling during 20 days, after the following treatments: G1- untreated; G2- treated with APF gel (1.23% F-, 4min); G3- irradiated with Nd:YAG (1064nm, 84.9J/ cm2, 10Hz) followed by APF application and G4- APF application before Nd:YAG laser irradiation (same conditions of G3). OCT (930 nm) analysis were performed before and after the treatments, 5th, 10th, 15th and 20th day of pH cycling. The optical attenuation coefficient decreased significantly (p<0.05) as demineralization increased over time and better results were achieved in group G4. OCT technique can diagnose early demineralization in enamel.
Longevity of diode laser irradiation in dentine
2018 - PEREIRA, DAISA L.; MORAES, MARCIA C.D.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
The efficacy of pulsed infrared lasers on clinical dentin wear sensitivity is well stablished in the Literature. This work aims to evaluate the effect of diode laser combined with fluoride products regarding the longevity of the sensibility treatment under the effect of the domestic use products. After Ethics Committee approval, 75 slabs of bovine root dentin measuring 8 mm2, were randomized into 5 groups (n=1 5): G1- untreated; G2- treated with acidulated phosphate fluoride (APF-gel, [F-J=1.23 %, pH=3.3 to 3.9); G3- irradiated with diode laser (980 nm, 2.5W, 5ms, 25Hz) using a cool paste as photoabsorber; G4- irradiated with diode laser (same conditions of G3) using a coal paste as photoabsorber + application of APF-gel and G5- application of APF-gel+ diode laser irradiation (same conditions of G3). All samples were submitted to an erosive and abrasive demineralization (Sprite Zero, pH=2.8, 90s, 4x/day), and remineralization (artificial saliva, pH=7.4) cycling for 10 days. Twice a day, slabs were brushed for 15s using a whitening dentifrice (Sensodyne True White, GSK, EUA). Slabs were evaluated by Optical Coherence Tomography (OCT) at the initial and 7th day of cycling. OCT measurements found a significant increase on dentin wear depth and area of lesions with the increase of cycling days. G1 and G2 hod significant higher mineral loss compared to the other groups. G3, G4 and GS showed no significant differences between them. Results suggested that the sensibility of the treatment with the diode laser has longevity despite the high abrasivity of dentifrice used in maintenance.
Gamma sterilized human dental enamel submitted to Er,Cr: YSGG laser irradiation associated to fluoride for in situ model
2018 - ZAMATARO, CLAUDIA B.; PEREIRA, DAISA de L.; LIMA, CASSIO A.; SCAPIN, MARCOS A.; ZEZELL, DENISE M.
Nd:YAG laser irradiation of darkened dental enamel by means of dentrifice use
2018 - PEREIRA, DAISA L.; GOMES, GABRIELA V.; SUGAHARA, VANESSA M.L.; SCAPIN, MARCOS A.; ANA, PATRICIA A.; ARANHA, ANA C.C.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
As the world population is growing older, dental erosion became a real concern on dentistry. Optical Coherence Tomography and X-Ray fluorescence were used to analyze enamel samples covered by black dentifrice as photoabsorber irradiated with NdYAG laser, then submitted to an erosive cycling. Heat injury risk in deeper tissue decreases because of the dentifrice dark pigment, when the most of photons are absorbed in the surface. Sixty Bmm2 blocks of bovine enamel, were randomized into 4 groups: G1- untreated; G2- treated with acidulated phosphate fluoride (APF, [FJ::1.23%, pH::3.3 to 3.9); G3- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz- Lares Research®) using a coal paste as photoabsorber; G4- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz), using a black dentifrice (Black is White, Curaprox®). All samples were submitted to a 3-day erosive demineralization (Citric acid 1%, pH::3.6, 5min, 2x/day) under agitation, and remineralization cycling (artificial saliva, pH::7, 120minJ. After treatments and pH cycling, the [Ca]/[P] ratio decreased in the laser irradiated groups. The samples were evaluated by OCT before and after treatment and after erosive cycling. OCT measurements found smaller lesion depths in laser irradiated groups than in the other groups and there were statistically significant differences between G3 and G4 groups (p<0.05). Heating promoted by Nd:YAG laser irradiation changed the morphological and crystallographic enamel surface properties and has shown to be a promissory alternative to prevent erosion.
Avaliação in vitro do teor de cálcio e fósforo em dentina humana submetida a radiação ionizante in vivo
2020 - JUNIOR, CLAUDIO M.; ZAMATARO, CLAUDIA B.; CARVALHO, VICTORIA G.; SILVA, MATEUS R.; ZEZELL, DENISE M.; SCAPIN, MARCOS A.; GONÇALVES, SERGIO E.P.
INTRODUÇÃO: Câncer de cabeça e pescoço é um problema de saúde pública. A radioterapia é uma opção de tratamento, capaz de promover maior preservação de tecidos e estruturas adjacentes. Entretanto, comorbidades ocorrem, causando alterações em tecidos da cavidade oral. O entendimento da etiologia das cáries de radiação ainda é um tema complexo no atendimento destes pacientes. OBJETIVO: Realizar a validação de uma metodologia de análise quantitativa in vitro de Fósforo (P) e Cálcio (Ca) na dentina humana submetida a radioterapia. METODOLOGIA: Após aprovação do Comitê de Ética em Pesquisa (parecer 3.842.547) foram utilizados 12 dentes extraídos oriundos de pacientes submetidos a radioterapia (~0,07 kGy) por câncer de cabeça e pescoço. As amostras de dentina foram preparadas e analisadas por meio de Microscopia Eletrônica de Varredura com Energia Dispersiva de Raios X (MEV/EDS). RESULTADOS: A metodologia proposta teve seus resultados comparados aos resultados obtidos pela espectroscopia de Fluorescência de Raios X e validada em termos de precisão e exatidão por meio de testes estatísticos sugeridos pelo documento DOQ-CGCRE-008 do INMETRO e teste Z-score. A precisão, em termos do desvio padrão relativo (RSD%) acessado de P e Ca, foi de 2,6 e 3,2%, respectivamente. A aceitabilidade calculada pelo teste de HORRAT (𝐻𝑂𝑅) mostrou que ambos valores são ≤ 2 (1,3 e 1,6 para P e CA, respectivamente), indicando que a precisão é satisfatória. A exatidão acessada de P e Ca, em termos de erro relativo (ER%), foi de 2,6 e 1,8%, respectivamente. A aceitabilidade, calculada pelo teste Z-score (score-score), apresentou valores entre 1,0 e 0,5 para P e CA, respectivamente, com a exatidão também considerada satisfatória. CONCLUSÃO: Conclui-se ser possível quantificar in vitro o Fósforo e o Cálcio na dentina humana por MEV/EDS, com parâmetros de precisão e exatidão satisfatórias para a técnica.
Molecular analysis of human and bovine hydroxyapatite from dental enamel and dentin submitted to gamma radiation
2018 - ZAMATARO, CLAUDIA B.; SCAPIN, MARCOS; KUCHAR, NIELSEN G.; ZANINI, NATHALIA; ZEZELL, DENISE M.
The important alteration of gamma irradiated hydroxyapatite in the buccal cavity depends on the biochemical alteration of the environment. The hydroxyapatite analyzed isolated showed a minimal molecular change of the crystal.
Esmalte dental sometido a radiación láser de Er:Cr:YSGG asociado con el uso de productos fluorados
2013 - ZAMATARO, CLAUDIA B.; ANA, PATRICIA A. da; BONETTI, CAROLINA; ZEZELL, DENISE M.
Association of fluoride and Er, Cr:YSGG laser irradiation provides enamel resistance to low pH environment
2013 - ZAMATARO, CLAUDIA B.; BENETTI, CAROLINA; ANA, PATRICIA A.; BLAY, ALBERTO; PEREIRA, THIAGO M.; ZEZELL, DENISE M.
Determinacao da difusividade termica em funcao da temperatura do esmalte e dentina bovinos
2009 - PEREIRA, T.M.; ANA, P.A.; ZAMATARO, C.B.; ZEZELL, D.M.