CLAUDIA BIANCHI ZAMATARO

CLAUDIA BIANCHI ZAMATARO

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In vivo study of diode laser 940 nm operating in high intensity mode in the periimplantitis disease
2018 - GOTTARDI, MARIELLA da S.; KAWAMOTO, DIONE; ZAMATARO, CLAUDIA B.; ANA, PATRICIA A. da; MAYER, MARCIA P.A.; ZEZELL, DENISE M.
The evolution of periimplantitis has been associated with several risk factors. The standard treatment consists on subgingival scaling and blasting with sodium bicarbonate. This 6 months longitudinal clinical study evaluated and compared two treatment methods: conventional as described and complemented by irradiation with high intensity diode laser (940 nm, Biolase) aiming to establish a more efficient periimplantitis management. After approval by the Human Research Ethics Committee, 20 patients of both sexes, with implants in function for at least 12 months and with diagnosis of periimplantitis were selected and randomly distributed in two experimental groups. Ten of them were conventionally treated as control group, and the remaining ten were in the laser treated group (1W, 4.24kJ/ cm2 energy density, pulsed mode, 30s, twice). There were six sites for treatment standardization: vestibular region (mesial, central and distal thirds) and buccal region (mesial, central and distal thirds). Results has shown that overall clinical evaluation has no significant difference between groups control and treatment. less bleeding and suppuration, as shallower periodontal pocket indicated a decreasing tendency on laser treated group. Quantitation of total bacteria and Porphyromona gingivalis by real-time PCR shown that laser synergy is a complementary therapy that could act as an additional option to the conventional treatment in difficult response cases.
Effect of Nd:YAG laser on caries prevention of dental enamel by optical attenuation coefficient with optical coherence tomography (OCT)
2018 - MORAES, MARCIA C.D.; PEREIRA, DAISA L.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.; FREITAS, ANDERSON Z.
Caries is a multifactorial disease, caused by the continuing loss of hydroxyapatite crystals. The association of topical acidulated phosphate fluoride application (APF) and high intensity lasers has the potencial to increase the resistance to demineralization of dental tissues. The Nd:YAG laser, specially associated to a photoabsorber over the dental surface, may induce thermal changes in order to increase the resistance to demineralization. Optical Coherence Tomography (OCT) is a non-ionizing radiation imaging technique that can be performed in real time in vivo and can detect early changes in the optical properties of t issues. This study aimed to characterize the effectiveness of the association of Nd:YAG la ser and APF gel in the prevention of caries lesions in human dental enamel in vitro, due to structural changes in function of the pH cycling, the treatments performed as well as to evaluate the potential of OCT to diagnose early demineralization. 160 human enamel samples were divided in four groups and submitted to pH cycling during 20 days, after the following treatments: G1- untreated; G2- treated with APF gel (1.23% F-, 4min); G3- irradiated with Nd:YAG (1064nm, 84.9J/ cm2, 10Hz) followed by APF application and G4- APF application before Nd:YAG laser irradiation (same conditions of G3). OCT (930 nm) analysis were performed before and after the treatments, 5th, 10th, 15th and 20th day of pH cycling. The optical attenuation coefficient decreased significantly (p<0.05) as demineralization increased over time and better results were achieved in group G4. OCT technique can diagnose early demineralization in enamel.
Longevity of diode laser irradiation in dentine
2018 - PEREIRA, DAISA L.; MORAES, MARCIA C.D.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
The efficacy of pulsed infrared lasers on clinical dentin wear sensitivity is well stablished in the Literature. This work aims to evaluate the effect of diode laser combined with fluoride products regarding the longevity of the sensibility treatment under the effect of the domestic use products. After Ethics Committee approval, 75 slabs of bovine root dentin measuring 8 mm2, were randomized into 5 groups (n=1 5): G1- untreated; G2- treated with acidulated phosphate fluoride (APF-gel, [F-J=1.23 %, pH=3.3 to 3.9); G3- irradiated with diode laser (980 nm, 2.5W, 5ms, 25Hz) using a cool paste as photoabsorber; G4- irradiated with diode laser (same conditions of G3) using a coal paste as photoabsorber + application of APF-gel and G5- application of APF-gel+ diode laser irradiation (same conditions of G3). All samples were submitted to an erosive and abrasive demineralization (Sprite Zero, pH=2.8, 90s, 4x/day), and remineralization (artificial saliva, pH=7.4) cycling for 10 days. Twice a day, slabs were brushed for 15s using a whitening dentifrice (Sensodyne True White, GSK, EUA). Slabs were evaluated by Optical Coherence Tomography (OCT) at the initial and 7th day of cycling. OCT measurements found a significant increase on dentin wear depth and area of lesions with the increase of cycling days. G1 and G2 hod significant higher mineral loss compared to the other groups. G3, G4 and GS showed no significant differences between them. Results suggested that the sensibility of the treatment with the diode laser has longevity despite the high abrasivity of dentifrice used in maintenance.
Gamma sterilized human dental enamel submitted to Er,Cr: YSGG laser irradiation associated to fluoride for in situ model
2018 - ZAMATARO, CLAUDIA B.; PEREIRA, DAISA de L.; LIMA, CASSIO A.; SCAPIN, MARCOS A.; ZEZELL, DENISE M.
Nd:YAG laser irradiation of darkened dental enamel by means of dentrifice use
2018 - PEREIRA, DAISA L.; GOMES, GABRIELA V.; SUGAHARA, VANESSA M.L.; SCAPIN, MARCOS A.; ANA, PATRICIA A.; ARANHA, ANA C.C.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.
As the world population is growing older, dental erosion became a real concern on dentistry. Optical Coherence Tomography and X-Ray fluorescence were used to analyze enamel samples covered by black dentifrice as photoabsorber irradiated with NdYAG laser, then submitted to an erosive cycling. Heat injury risk in deeper tissue decreases because of the dentifrice dark pigment, when the most of photons are absorbed in the surface. Sixty Bmm2 blocks of bovine enamel, were randomized into 4 groups: G1- untreated; G2- treated with acidulated phosphate fluoride (APF, [FJ::1.23%, pH::3.3 to 3.9); G3- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz- Lares Research®) using a coal paste as photoabsorber; G4- irradiated with Nd:YAG laser (1064nm, 0.6W, 10Hz), using a black dentifrice (Black is White, Curaprox®). All samples were submitted to a 3-day erosive demineralization (Citric acid 1%, pH::3.6, 5min, 2x/day) under agitation, and remineralization cycling (artificial saliva, pH::7, 120minJ. After treatments and pH cycling, the [Ca]/[P] ratio decreased in the laser irradiated groups. The samples were evaluated by OCT before and after treatment and after erosive cycling. OCT measurements found smaller lesion depths in laser irradiated groups than in the other groups and there were statistically significant differences between G3 and G4 groups (p<0.05). Heating promoted by Nd:YAG laser irradiation changed the morphological and crystallographic enamel surface properties and has shown to be a promissory alternative to prevent erosion.
SEM/EDS for determining the phosphorus and calcium content in human dentin submitted to radiotherapy for head and neck cancer
2020 - SCAPIN, MARCOS A.; MOREIRA JUNIOR, CLAUDIO; ZAMATARO, CLAUDIA B.; GONCALVES, SERGIO E. de P.; ZEZELL, DENISE M.
The research followed the CONSORT 2010 guidelines. Patients who underwent radiotherapy due to the head and neck cancer (n = 12) and healthy patients (n = 12) donated the teeth. Twenty-four urgently extracted teeth for periodontal reasons or in order to prevent infectious processes were used. All teeth obtained had the approval from the ICT - UNESP Human Experiments Ethics Committee. The teeth obtained were separated into two large groups: Control (n = 12 dentin teeth of healthy patients) and Irradiated (n = 12 dentin teeth of patients irradiated in vivo) and sectioned in their long axes, obtaining two halves (n = 24 per group). The samples were analyzed by Scanning Electron Microscopy with Energy Dispersive (SEM/EDS) and Surface Microhardness (SMH). The results obtained by SEM/EDS and X-Ray Fluorescence techniques were analyzed separately and then compared to each other. To validate the analytical method of the correlation between the two types of tests performed, the Horwitz equation was used, which would generate a Horwitz value and establishes for either method. Precision for repeatability was assessed using the relative percentage standard deviation (RDS%) and the acceptability criterion by the Horwitz test. Accuracy was assessed by relative percentage error (ER%) and acceptance criterion by z-score. The precision for repeatability was evaluated through the relative standard deviation (RSD%) and the acceptability was verified using the equation of Horwitz, in which the HORRAT’s (HOR) values fit more accordingly.
Comportamento da hidroxiapatita do esmalte e da dentina frente à radiação ionizante in vivo e in vitro
2019 - KUCHAR, NIELSEN G.; ZAMATARO, CLAUDIA B.; ZANINI, NATHALIA; RABELO, THAIS F.; JUVINO, AMANDA C.; SOGLIA, VICTOR; CASTRO, PEDRO A.A.; ZEZELL, DENISE
Avaliação in vitro do teor de cálcio e fósforo em dentina humana submetida a radiação ionizante in vivo
2020 - JUNIOR, CLAUDIO M.; ZAMATARO, CLAUDIA B.; CARVALHO, VICTORIA G.; SILVA, MATEUS R.; ZEZELL, DENISE M.; SCAPIN, MARCOS A.; GONÇALVES, SERGIO E.P.
INTRODUÇÃO: Câncer de cabeça e pescoço é um problema de saúde pública. A radioterapia é uma opção de tratamento, capaz de promover maior preservação de tecidos e estruturas adjacentes. Entretanto, comorbidades ocorrem, causando alterações em tecidos da cavidade oral. O entendimento da etiologia das cáries de radiação ainda é um tema complexo no atendimento destes pacientes. OBJETIVO: Realizar a validação de uma metodologia de análise quantitativa in vitro de Fósforo (P) e Cálcio (Ca) na dentina humana submetida a radioterapia. METODOLOGIA: Após aprovação do Comitê de Ética em Pesquisa (parecer 3.842.547) foram utilizados 12 dentes extraídos oriundos de pacientes submetidos a radioterapia (~0,07 kGy) por câncer de cabeça e pescoço. As amostras de dentina foram preparadas e analisadas por meio de Microscopia Eletrônica de Varredura com Energia Dispersiva de Raios X (MEV/EDS). RESULTADOS: A metodologia proposta teve seus resultados comparados aos resultados obtidos pela espectroscopia de Fluorescência de Raios X e validada em termos de precisão e exatidão por meio de testes estatísticos sugeridos pelo documento DOQ-CGCRE-008 do INMETRO e teste Z-score. A precisão, em termos do desvio padrão relativo (RSD%) acessado de P e Ca, foi de 2,6 e 3,2%, respectivamente. A aceitabilidade calculada pelo teste de HORRAT (𝐻𝑂𝑅) mostrou que ambos valores são ≤ 2 (1,3 e 1,6 para P e CA, respectivamente), indicando que a precisão é satisfatória. A exatidão acessada de P e Ca, em termos de erro relativo (ER%), foi de 2,6 e 1,8%, respectivamente. A aceitabilidade, calculada pelo teste Z-score (score-score), apresentou valores entre 1,0 e 0,5 para P e CA, respectivamente, com a exatidão também considerada satisfatória. CONCLUSÃO: Conclui-se ser possível quantificar in vitro o Fósforo e o Cálcio na dentina humana por MEV/EDS, com parâmetros de precisão e exatidão satisfatórias para a técnica.
Heat-induced depth of Nd:YAG laser irradiation in biological hard tissues
2019 - PEREIRA, DAISA L.; CASTRO, PEDRO A.A.; GOMES, GABRIELA V.; ZAMATARO, CLAUDIA B.; ZEZELL, DENISE M.; RIBEIRO, HENRIQUE B.; MATOS, CHRISTIANO J.S.; ANA, PATRICIA A.
Confocal Raman spectroscopy is a non-destructive and non-invasive technique which provides surface Raman spectra and depth images of biological structures contactless with the sample, with no use of ionizing radiation to penetrate in the sample under analysis. These characteristics allow its experimental use without any side e®ects to the sample. The depth images are obtained by Raman microscopy and are related to the characteristics of the tissues. This study aims to characterize irradiated hard tissues and correlate the depth reached by the heat of the laser irradiation with the obtained images. For this, thirty 8 mm2 blocks of bovine enamel and bovine root dentin, were randomized into 6 groups: G1- enamel untreated; G2- enamel irradiated with Nd:YAG micropulsed laser (1064 nm, 10 Hz- Lares Research R°) using a coal paste as photoabsorber; G3- enamel irradiated with Nd:YAG nanopulsed laser (1064 nm, 20 Hz, Brilliant, Quantel Laser) using a coal paste as photoabsorber; G4-G6 (bovine root dentin in the same conditions of treatment of G1-G3). The measurements were performed in three di®erent depth regions of the cubic shaped samples: region A- left corner above of the sample, region B- middle of the sample and Region C- right corner below of the sample. The area under the phosphate, carbonate, amide I, II, and III bands were calculated. The Raman spectra of the Nd:YAG irradiated samples detected a reduction in all the organic components of the enamel after laser irradiation. Previous studies of our group demonstrated that di®erences in carbonate substitution in the apatite lattice are related to the apatite instability and demineralization susceptibility. Considering that carbonate free apatite is less susceptible to acid attack, the results of this study suggest that Nd:YAG lased enamel can be more resistant to caries, in a direct correlation to the thickness of the treated area. It was found that for micropulsed Nd:YAG laser, the heat induced depth was 10 § 2 ¹m and for nanopulsed laser the heat induced depth was 8 § 3 ¹m. So, it is possible to correlate the heat penetration depth of the laser irradiation with the images obtained by the confocal Raman.
Calcium analysis from gamma sterilized human dentin and enamel
2019 - ZAMATARO, CLAUDIA B.; KUCHAR, NIELSEN G.; SCAPIN, MARCOS A.; ZANINI, NATHALIA; CASTRO, PEDRO; RABELO, THAIS F.; JUVINO, AMANDA C.; ZEZELL, DENISE
Gamma radiation changes the patients0 oral cavity undergoing radiotherapy. Alterations cause an unsaturated environment of calcium and phosphate into the oral cavity. After approval of the Ethics Committee, 20 hu- man teeth were sectioned to obtain 20 human enamel and 20 dentin samples, polished plane. Samples were randomized in the irradiated group and control group (untreated). Then, the treatment group was irradiated with 25:0 kGy at the 60Co multipurpose irradiator. After the gamma irradiation, Fourier Transformed Infrared Spectroscopy (FTIR), percentage of surface microhardness loss (%SMHL) and Scanning Electron Microscopy (SEM) were performed. At the end, acidic biopsies were performed to quantify the concentration of calcium present in the samples. FTIR showed that the molecular structure of HA of the enamel is similar to the non- irradiated, with no formation or loss of molecular compounds occurring. X-ray °uorescence at enamel samples was performed. Microscopic morphological analysis did not shown signi¯cant di®erences. Surface microhardness is an indirect indicator of the mineral content of the samples. The mean obtained was 258:2 (38:8) KHN within the hardness spectrum of the healthy natural enamel. The compounds present in the samples and the values of the ratios of Calcium and Phosphate oxides and relation between the elements Calcium and Phosphorus. The ratio of the most stable oxides shows a variation with linear correlation. In the enamel, the ratio (Ca/P) shows a change in the elemental content with linear correlation (R2 = 1). These ¯ndings lead us to a new hypothesis of behaviour of the HA crystal versus gamma irradiation. On the other hand for the irradiated dentin, the Knoop hardness number was within the range of the spectrum similar to that of natural dentin of human origin. X-ray °uorescence shows that irradiated dentin has great similarity with natural dentin from the point of view of chemical composition. SEM analyses showed that there was no thermal damage or interprismatic morpho- logical changes in the hydroxyapatite structure of human dental dentin outside the buccal environment when using doses of gamma irradiation up to 25 kGy.