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Resumo IPEN-doc 29546 Photobiomodulation therapy as a radiosensitizer for triple-negative breast cancer2022 - SILVA, CAMILA R.; PEREIRA, SAULO de T.; PRETTO, LUCAS R. de; FREITAS, ANDERSON Z. de; RIBEIRO, MARTHA S.INTRODUCTION: Radiotherapy (RT) is an essential cancer treatment and is estimated that approximately 52% of oncological patients will be submitted to this technique once. However, some tumors, such as triple-negative breast cancer (TNBC), present radioresistance, demanding high doses of ionizing radiation (IR) and a prolonged period of treatment, which contributes to secondary malignancies due to deposition of dose in organs at risk and several side effects. Moreover, this subtype of cancer shows a high incidence of metastasis and decreases the survival expectancy of the patient. Thus, the search for new agents that can act as a radiosensitizer to improve the RT effects has been growing. Conversely, photobiomodulation therapy (PBM), which is a promising therapy with increasing adhesion in clinical practice, has been used to mitigate the adverse effects of RT. Indeed, recent studies have associated PBM with RT to combat cancer. OBJECTIVES: In this study, we used TNBC-bearing mice as a radioresistant cancer model to verify if PBM could act as a radiosensitizer MATERIALS AND METHODS: PBM was applied in two different protocols before the RT with a high dose (60 Gy fractioned in 4 sessions). We evaluated the tumor volume progression, animal clinical evolution, lung metastases by optical coherence tomography, and animal survival DISCUSSION AND RESULTS: Our data indicate that PBM before each RT session arrested the tumor volume, improved the clinical signals of the animals, reduced the nodules in the lung, and extended animal survival. CONCLUSION: In the light of the knowledge gained, our data indicate that PBM could act as a radiosensitizer.Resumo IPEN-doc 27949 Laser speckle imaging for osteoporosis evaluation2020 - AMARAL, MARCELLO M.; DEL-VALLE, MATHEUS; RAELE, MARCUS P.; PRETTO, LUCAS R. deOsteoporosis is a common disease characterized by the reduction on Bone Mineral Density (BMD), leading to weakening of bone structure, Chronic pain, deformities and loss of quality of life. In addition to the clinical evaluation, dual-energy X-ray absorptiometry is one of the main techniques to diagnose it. However, this technique uses ionizing radiation to assess the bone structure and therefore cannot be used very often by patients, due to radiological safety reasons. On the other hand, optical techniques are known for its safe use, due to non-ionizing radiation, however, optical techniques do not easily allows the analysis of bone tissue. This limitation could be circumvented in the oral cavity area. In this work we used the Laser Speckle Imaging (LSI) technique to evaluate maxilla and mandible bones after demineralization prosses in an animal in vitro model. Osteoporosis lesions were simulated in sixteen mandible and twelve maxilla slabs using Ethylenediaminetetraacetic Acid (EDTA) 0.5 M for 0 (control) 7, 15 and 30 days. The roughness parameters Ra and Rq were analyzed with optical profilometry (ZeGage, Zygo, USA) to characterize the demineralization process. The LSI images were measured by custom experimental setup. A collimated laser beam at 635 nm and 1.3mW (Thorlabs CPS635R), expanded by a diverging lens (-75 mm), illuminates the sample. The scattered signal was imaged by a CCD camera (Thorlabs - DCC1645-HQ), an adapter (Thorlabs MVLCMC) and objective lens (Thorlabs/Navitar - MVL12X3Z) setting. A custom software was implemented to measure the speckle patches ratio and the speckle contrast ratio from speckle images obtained by a custom LSI setup. The speckle contrast ratio method only differentiate sound from osteoporotic tissue. The speckle patches ratio method presented a negative correlation with the roughness parameter, and consequently with the demineralization level. It was concluded that LSI is a promissory technique for assessment osteoporosis lesions on alveolar bone and, for that, the patches ratio is the best methodology for detecting and differentiating several degrees of demineralization.Resumo IPEN-doc 27450 Assessment of osteoporosis through laser speckle imaging2020 - AMARAL, M.M.; DEL-VALLE, M.; RAELE, M.P.; PRETTO, L.R. de; ANA, P.A.