JANAINA BAPTISTA ALVES
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Artigo IPEN-doc 14475 Immunological assessment of mice hyperimmunized with native and cobalt-60-irradiated bothrops venoms2005 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; MARTINEZ, J.C.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 14476 Young ovine death during hyperimmunization: crotalic envenomation or copper toxicosis?2008 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 14474 Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera2005 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; MARTINEZ, J.C.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 13015 Laboratory evaluation of young ovines inoculated with natural or sup(60)Co-irradiated Crotalus durissus terrificus venom during hyperimmunization process2006 - FERREIRA JUNIOR, R.S.; NASCIMENTO, N.; COUTO, R.; ALVES, J.B.; MEIRA, D.A.; BARRAVIERA, B.Artigo IPEN-doc 11620 Immune response against irradiated with sup(60)Co gamma-rays2006 - BAPTISTA, J.A.; SPENCER, P.J.; OLIVEIRA, J.E.; CESARE, M.S.; NASCIMENTO, N.Artigo IPEN-doc 10991 A morphological in vitro study of the effects of Nd:YAG laser on irradiated cervical dentin2004 - MAGALHAES, M.F.; MATSON, E.; ROSSI, W.; ALVES, J.B.Artigo IPEN-doc 09897 Effects of gamma rays on the immunogenicity (IgG types) of ovalbumin2004 - BAPTISTA, J.A.; SPENCER, P.J.; AROEIRA, L.G.S.; CASARE, M.S.; NASCIMENTO, N.Artigo IPEN-doc 09896 Effects of sup(60)Co radiation on the molecular structure of crotamine2004 - CASARE, M.S.; BAPTISTA, J.A.; SPENCER, P.J.; NASCIMENTO, N.Artigo IPEN-doc 14083 Study of irradiated bothropstoxin-1 with sup(6)Co gamma rays: immune system behavior2009 - CAPRONI, P.; BAPTISTA, J.A.; ALMEIDA, T.L. de; PASSOS, L.A.C.; NASCIMENTO, N.Ionizing radiation has been successfully employed to modify the immunological properties of biomolecules. Very promising results were obtained when crude animal venoms, as well as isolated toxins, were treated with 60Co gamma rays, yielding toxoids with good immunogenicity. The achievement of modified antigens with lower toxicity and preserved or improved immunogenicity can be very useful. Ionizing radiation has already been proven to be a powerful tool to attenuate snake venom toxicity without affecting, and even increasing, their immunogenic properties. However, little is known about the modifications that irradiated molecules undergo and even less about the immunological response that such antigens elicit. In the present work, we investigated the immunological behavior of bothropstoxin-1, a K49 phospholipase, before and after irradiation. Structural modifications of the toxin were analyzed by SDS-PAGE. Isogenic mice were immunized with either the native or the irradiated toxin. The circulating antibodies were isotyped and titrated by ELISA. According to our data, irradiation promoted structural modifications in the toxin characterized by higher molecular weight forms of proteins (aggregates and oligomers). The results also indicated that irradiated toxins were immunogenic and antibodies elicited by them were able to recognize the native toxin in ELISA. These findings suggest that irradiation of toxic proteins can promote significant modifications in their structures; however they still retain many of the original antigenic and immunological properties of native proteins. Also, our data indicate that irradiated proteins induce higher titers of IgG2a and IgG2b, suggesting that Th1 cells are predominantly involved in the immune response.Artigo IPEN-doc 14173 Immunological properties of sup(60)Co gamma-rays irradiated bothropstoxin-I2009 - BAPTISTA, J.A.; VIEIRA, D.P.; GALISTEO JUNIOR, A.J.; CAPRONI, P.; CASARE, M.; ANDRADE JUNIOR, H.F. de; SPENCER, P.J.; NASCIMENTO, N.