AECIO MASSAYOSHI YAMADA JUNIOR

AECIO MASSAYOSHI YAMADA JUNIOR

Página do item completo

Resultados de Busca

Agora exibindo 1 - 6 de 6

Bactericidal effect of malachite green and red laser on Antinobacillus actinomycetemcomitans
2007 - PRATES, RENATO A.; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; HASHIMOTO, MARIA C.E.; CAI, SILVANA; SOARES, SHEILA G.; GOMES, LAERCIO; RIBEIRO, MARTHA S.
Comparative study between the effects of photodynamic therapy and conventional therapy on microbial reduction in ligature-induced peri-implantitis in dogs
2005 - HAYEK, R.R.A.; ARAUJO, N.S.; GIOSO, M.A.; FERREIRA, J.; BAPTISTA SOBRINHO, C.A.; YAMADA JUNIOR, A.M.; RIBEIRO, M.S.
The irradiation parameters investigation of photodynamic therapy on yeast cells
2008 - PRATES, RENATO A.; SILVA, ERIQUES G. da; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; PAULA, CLAUDETE R.; RIBEIRO, MARTHA S.
It has been proposed that photodynamic therapy (PDT) can inactivate microbial cells. A range of photosensitizers and light sources were reported as well as different fluence parameters and dye concentrations. However, much more knowledge regarding to the role of fluences, irradiation time and irradiance are required for a better understanding of the photodynamic efficiency. The aims of this study were to investigate the role of light parameters on the photoinactivation of yeast cells, and compare cell survivors in different growing phases following PDT. To perform this study, a suspension (106cfu/mL) of Candida albicans ATCC-90028 was used in log and stationary-phase. Three irradiances 100mW/cm2, 200mW/cm2 and 300mW/cm2 were compared under 3min, 6min and 9min of irradiation, resulting in fluences of 18, 36, 54, 72,108 and 162J/cm2. The light source used was a laser emitting at 660nm with output power of 30, 60 and 90mW. As photosensitizer, 100μΜ methylene blue was used. PDT was efficient against yeast cells (6 log reduction) in log and stationary-phase. Neither photosensitizer nor light alone presented any reduction of cell viability. The increase of irradiance and time of irradiation showed a clearly improvement of cell photoinactivation. Interestingly, the same fluences in different irradiances presented dissimilar effects on cell viability. The irradiance and time of irradiation are important in PDT efficiency. Fluence per se is not the best parameter to compare photoinativation effects on yeast cells. The growing-phases presented the same susceptibility under C. albicans photoinactivation.
Photosensitization of Aggregatibacter actinomycetemcomitans with methylene blue: A microbiological and spectroscopic study
2008 - YAMADA JUNIOR, AECIO; PRATES, RENATO A.; CAI, SILVANA; RIBEIRO, MARTHA S.
The aim of this study was to determinate the efficiency of methylene blue (MB) to kill cultures of Aggregatibacter actinomycetemcomitans under red light and to investigate MB photobleaching by optical absorption spectroscopy. Bacteria were diluted in aqueous solution, putted in glass tubes and distributed in 5 groups: (L-MB-) control group; (L+MB-) laser alone by 5min; (L-MB+) MB alone through 5min; (3L+MB+) MB+laser 3min; (5L+MB+) MB+laser 5min. Laser parameters were P=30mW, λ=660nm, E=9J in 5min and E=5.4J in 3min. The samples were diluted and bacterial colonies were counted and converted into colony forming units (CFU). Absorption spectra of the MB-stained bacterial suspension and photosensitized bacterial suspension were obtained. Groups L-MB-, L+MB-, and L-MB+ did not show a decrease in CFU/mL. L+MB+ groups showed a significant decrease in CFU/mL but no statistically significant differences were observed between 3min and 5min. Spectroscopy showed that MB is photodegraded after irradiation and that dimer species are more notably consumed than monomeric species. These results suggest that MB is a suitable photosensitizer to reduce A. actinomycetemcomitans, and that 3min of irradiation are enough to produce a significant effect. Due to the spectral changes observed on MB solution after irradiation a type I mechanism may be involved.
Light parameters influence cell viability in antifungal photodynamic therapy in a fluence and rate fluence-dependent manner
2009 - PRATES, RENATO A.; SILVA, ERIQUES G. da; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; PAULA, CLAUDETE R.; RIBEIRO, MARTHA S.
Histomorphometric and microbial assessment of photodynamic therapy as an adjuvant treatment for periodontilis: A short-term evaluation of inflammatory periodontal conditions and bacterial reduction in a rat model
2011 - PRATES, RENATO A.; YAMADA JUNIOR, AECIO M.; SUZUKI, LUIS C.; FRANÇA, CRISTIANE M.; CAI, SILVANA; MAYER, MARCIA P.A.; RIBEIRO, ADRIANA C.; RIBEIRO, MARTHA S.