NÉLIO ALESSANDRO DE JESUS OLIVEIRA
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Artigo IPEN-doc 27126 Phenotype changes of oral epithelial stem cells after in vitro culture2020 - DALTOE, FELIPE P.; OLIVEIRA, NÉLIO A.J. de; PERONI, CIBELE N.; SHARPE, PAUL T.; MANTESSO, ANDREAThe aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on the expression of the epithelial stem cell markers p75NTR and CD71. We also examined the co-expression of other epithelial stem markers such as integrins β1 and α6 and their stem cell-like proprieties in in vitro assays. Three passages after being sorted by MACS, more than 93% of the p75NTR+ve cells lost the expression of p75NTR, while 5.46% of the p75NTR-ve gained it. Within the small population of the p75NTR+ve cells, 88% co-expressed other epithelial stem cell markers such as integrins β1 and α6, while only 28% of p75NTR-ve cells co-expressed these markers. These results were confirmed by sorting cells by FACS. Additionally, when double staining was used for sorting cells, 99% of the p75NTR+veCD71-ve and 33% of the p75NTR-veCD71+ve cells expressed both integrins, but just one week after culture, only 1.74% of the p75NTR+veCD71-ve cells still expressed p75NTR and only 0.32% still expressed CD71. Similar results were obtained when co-culturing p75NTR+ve and p75NTR-ve populations before analysis. Our results suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body. In addition, in vitro culture may not offer ideal conditions for epithelial stem cell maintenance due to phenotype changes under standard culture conditions.Artigo IPEN-doc 21730 Partial correction of the dwart phenotype by non-viral transfer of the growth hormone gene in mice: treatment age is critical2016 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; OLIVEIRA, NELIO A.J.; LIMA, ELIANA R.; VIEIRA, DANIEL P.; AAGAARD, LARS; JENSEN, THOMAS G.; JORGE, ALEXANDER A.L.; BARTOLINI, PAOLO; PERONI, CIBELE N.Capítulo IPEN-doc 17391 Different ex vivo and direct in vivo DNA administration strategies for growth hormone gene therapy in dwarf animals2011 - PERONI, CIBELE N.; OLIVEIRA, NELIO A. de J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; BARTOLINI, PAOLOResumo IPEN-doc 17505 In vitro, ex vivo and in vivo recombinant growth hormone synthesis, for gene therapy applications2011 - PERONI, CIBELE N.; OLIVEIRA, NELIO A. de J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; SILVA, JULIANA T. da; JORGE, ALEXANDER A. de L.; JENSEN, THOMAS G.; BARTOLINI, PAOLOResumo IPEN-doc 17526 Growth parameters after intramuscular hGH plasmid administration compared to recombinant hGH injections in LIT/SCID mice2011 - HIGUTI, E.; CECCHI, C.R.; OLIVEIRA, N.A.J.; SILVA, J.T.; JORGE, A.A.L.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16658 Assessment of the in vivo permanence of mGH-secreting human keratinocytes in grafted organotypic cultures2009 - CECCHI, C.R.; OLIVEIRA, N.A.J.; HIGUTI, E.; NONOGAKI, S.; BOCCARDO, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16659 Electrotransfer of naked hGH cDNA muscle of lit/scid mouse is more efficient than genomic DNA2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; MORISCOT, A.S.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16651 Sustained hGH expression after electrotransfer of naked DNA into DWARF 'little!' mouse skeletal muscle2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16636 Enriquecimento de queratinócitos secretores de mGH em células-tronco mediante adesão rápida ao colágeno2010 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16421 Growth parameters after intramuscular hGH plasmid administration compared to recombinant hGH injections in lit/scid mice2011 - HIGUTI, E.; CECCHI, C.R.; OLIVEIRA, N.A.J.; SILVA, J.T.; JORGE, A.A.L.; BARTOLINI, P.; PERONI, C.N.