NÉLIO ALESSANDRO DE JESUS OLIVEIRA
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Artigo IPEN-doc 23455 Molecular cloning and characterization of pirarucu (Arapaima gigas) follicle-stimulating hormone and luteinizing hormone β-subunit cDNAs2017 - SEVILHANO, THAIS; CARVALHO, ROBERTO F. de; OLIVEIRA, NELIO A. de J.; OLIVEIRA, JOAO E.; MALTAROLLO, VINICIUS G.; TROSSINI, GUSTAVO; GARCEZ, RIVIANE; BARTOLINI, PAOLOThe common gonadotrophic hormone α-subunit (GTHα) has been previously isolated by our research group from A. gigas pituitaries; in the present work the cDNA sequences encoding FSHβ and LHβ subunits have also been isolated from the same species of fish. The FSH β-subunit consists of 126 amino acids with a putative 18 amino acid signal peptide and a 108 amino acid mature peptide, while the LH β-subunit consists of 141 amino acids with a putative 24 amino acid amino acid signal peptide and a 117 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the order of Anguilliformes (61%) for FSHβ and of Cypriniformes (76%) for LHβ, followed by Siluriformes, 53% for FSHβ and 75% for LHβ. Interestingly, the identity with the corresponding human amino acid sequences was still remarkable: 45.1% for FSHβ and 51.4% for LHβ. Three dimensional models of ag-FSH and ag-LH, generated by using the crystal structures of h-FSH and h-LH as the respective templates and carried out via comparative modeling and molecular dynamics simulations, suggested the presence of the so-called ªseat-beltº, favored by a disulfide bond formed between the 3rd and 12th cysteine in both β-subunits. The sequences found will be used for the biotechnological synthesis of A. gigas gonadotrophic hormones (ag-FSH and ag-LH). In a first approach, to ascertain that the cloned transcripts allow the expression of the heterodimeric hormones, ag-FSH has been synthesized in human embryonic kidney 293 (HEK293) cells, preliminarily purified and characterized.Artigo IPEN-doc 21730 Partial correction of the dwart phenotype by non-viral transfer of the growth hormone gene in mice: treatment age is critical2016 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; OLIVEIRA, NELIO A.J.; LIMA, ELIANA R.; VIEIRA, DANIEL P.; AAGAARD, LARS; JENSEN, THOMAS G.; JORGE, ALEXANDER A.L.; BARTOLINI, PAOLO; PERONI, CIBELE N.Resumo IPEN-doc 20291 The influence of Escherichia coli cultivation temperature on interferon alpha 2a expression (IFN-'alfa'2a)2014 - ARTHUSO, FERNANDA dos S.; SUZUKI, MIRIAM F.; OLIVEIRA, NELIO A. de J.; OLIVEIRA, JOAO E. de; BARTOLINI, PAOLO; SOARES, CARLOS R.J.Capítulo IPEN-doc 17391 Different ex vivo and direct in vivo DNA administration strategies for growth hormone gene therapy in dwarf animals2011 - PERONI, CIBELE N.; OLIVEIRA, NELIO A. de J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; BARTOLINI, PAOLOResumo IPEN-doc 17505 In vitro, ex vivo and in vivo recombinant growth hormone synthesis, for gene therapy applications2011 - PERONI, CIBELE N.; OLIVEIRA, NELIO A. de J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; SILVA, JULIANA T. da; JORGE, ALEXANDER A. de L.; JENSEN, THOMAS G.; BARTOLINI, PAOLOResumo IPEN-doc 17526 Growth parameters after intramuscular hGH plasmid administration compared to recombinant hGH injections in LIT/SCID mice2011 - HIGUTI, E.; CECCHI, C.R.; OLIVEIRA, N.A.J.; SILVA, J.T.; JORGE, A.A.L.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16658 Assessment of the in vivo permanence of mGH-secreting human keratinocytes in grafted organotypic cultures2009 - CECCHI, C.R.; OLIVEIRA, N.A.J.; HIGUTI, E.; NONOGAKI, S.; BOCCARDO, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16659 Electrotransfer of naked hGH cDNA muscle of lit/scid mouse is more efficient than genomic DNA2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; MORISCOT, A.S.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16651 Sustained hGH expression after electrotransfer of naked DNA into DWARF 'little!' mouse skeletal muscle2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16636 Enriquecimento de queratinócitos secretores de mGH em células-tronco mediante adesão rápida ao colágeno2010 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; BARTOLINI, P.; PERONI, C.N.
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