SILVIA CRISTINA NUNEZ
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Artigo IPEN-doc 23565 Exploring the effects of low-level laser therapy on fibroblasts and tumor cells following gamma radiation exposure2016 - SILVA, CAMILA R.; CABRAL, FERNANDA V.; CAMARGO, CLAUDINEI F.M. de; NUNEZ, SILVIA C.; YOSHIMURA, TANIA M.; LUNA, ARTHUR C. de L.; MARIA, DURVANEI A.; RIBEIRO, MARTHA S.Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm–2, 90 J cm–2, and 150 J cm–2. Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes – in a fluence-dependent manner – increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G2/M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4th day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.Artigo IPEN-doc 21364 PDT in oral diseases of microbial origin: The challenge of oral biofilm2015 - NUNEZ, SILVIA C.Artigo IPEN-doc 11895 Antimicrobial photodynamic therapy combined with conventional endodontic treatment to eliminate root canal biofilm infection2007 - GARCEZ, AGUINALDO S.; RIBEIRO, MARTHA S.; TEGOS, GEORGE P.; NUNEZ, SILVIA C.; JORGE, ANTONIO O.C.; HAMBLIN, MICHAEL R.Artigo IPEN-doc 10832 He-Ne laser effects on blood microcirculation during wound healing: a method of in vivo study through laser doppler flowmetry2004 - NUNEZ, S.C.; NOGUEIRA, G.E.C.; RIBEIRO, M.S.; GARCEZ, A.S.; MARQUES, J.L.LArtigo IPEN-doc 11592 Collagen birefringence in skin repair in response to red polarized-laser therapy2006 - SILVA, DANIELA de F.T. da; VIDAL, BENEDICTO de C.; ZEZELL, DENISE M.; ZORN, TELMA M.T.; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.Artigo IPEN-doc 18050 Antimicrobial photodynamic therapy on drug-resistant Pseudomonas aeruginosa-induced infection. An in vivo study2012 - HASHIMOTO, MARIA C.E.; PRATES, RENATO A.; KATO, ILKA T.; NUNEZ, SILVIA C.; COURROL, LILIA C.; RIBEIRO, MARTHA S.