FABIANO YOKAICHIYA
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Artigo IPEN-doc 27384 Capsaicin-cyclodextrin complex enhances mepivacaine targeting and improves local anesthesia in inflamed tissues2020 - COUTO, VERONICA M.; OLIVEIRA-NASCIMENTO, LAURA de; CABEÇA, LUIZ F.; GERALDES, DANILO C.; COSTA, JULIANA S.R.; RISKE, KARIN A.; FRANZ-MONTAN, MICHELLE; YOKAYCHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; PAULA, ENEIDA deAcidic environments, such as in inflamed tissues, favor the charged form of local anesthetics (LA). Hence, these drugs show less cell permeation and diminished potency. Since the analgesic capsaicin (CAP) triggers opening of the TRPV1 receptor pore, its combination with LAs could result in better uptake and improved anesthesia. We tested the above hypothesis and report here for the first time the analgesia effect of a two-drug combination (LA and CAP) on an inflamed tissue. First, CAP solubility increased up to 20 times with hydroxypropyl-beta-cyclodextrin (HP-β-CD), as shown by the phase solubility study. The resulting complex (HP-β-CD-CAP) showed 1:1 stoichiometry and high association constant, according to phase-solubility diagrams and isothermal titration calorimetry data. The inclusion complex formation was also confirmed and characterized by differential scanning calorimetry (DSC), X-ray diffraction, and 1H-NMR. The freeze-dried complex showed physicochemical stability for at least 12 months. To test in vivo performance, we used a pain model based on mouse paw edema. Results showed that 2% mepivacaine injection failed to anesthetize mice inflamed paw, but its combination with complexed CAP resulted in pain control up to 45 min. These promising results encourages deeper research of CAP as an adjuvant for anesthesia in inflamed tissues and cyclodextrin as a solubilizing agent for targeting molecules in drug delivery.Artigo IPEN-doc 26865 Complexation of the local anesthetic pramoxine with hydroxypropyl-beta-cyclodextrin can improve its bioavailability2020 - BEZAMAT, JULIANA M.; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; CASTRO, SIMONE R.; PAULA, ENEIDA de; CABEÇA, LUIS F.Local anesthetics are widely used in clinical procedures, to eliminate pain during/after invasive procedures. A wide range of drug delivery systems has been developed to improve the effect of local anesthetics and/or to reduce their toxicity. Pramoxine (PMX) is a topical anesthetic agent with an unusual (morpholine ring) structure and low solubility (ca. 3 mM at pH 7.4). In this work, a novel formulation was devised for PMX in hydroxypropyl- β-cyclodextrin (HP-β-CD). Host-guest inclusion complex was prepared by the co-solubilization method, with complexation kinetics of 10 h, and 1:1 PMX/HP-β-CD stoichiometry. Complexation promoted 14-fold increase in the solubility of PMX. X-ray diffraction measurements revealed loss of the crystalline PMX pattern in the presence of HP-β-CD, an indication of inclusion complexation. Using 1H NMR (DOSY) experiments the association constant of PMX to HP-β-CD (Ka = 923.1 mol/L) was determined, while nuclear Overhauser (ROESY) analysis confirmed the formation of PMX/HP-β-CD inclusion complex, by detection of spatial proximities between hydrogens from PMX aromatic ring and cyclodextrin's cavity. In two in vitro toxicity models (mouse 3T3 fibroblasts in culture and red blood cells hemolysis) pramoxine toxicity was significantly reduced upon complexation into HP-β-CD. These results point out PMX/HP-β-CD as a promising pharmaceutical formulation to improve the bioavailability of pramoxine, allowing its application beyond topical anesthesia.Artigo IPEN-doc 22552 Tuning ferroic states in La doped BiFeOsub(3)-PbTiOsub(3) displacive multiferroic compounds2014 - COTICA, L.F.; FREITAS, V.F.; PROTZEK, O.A.; EIRAS, J.A.; GARCIA, D.; YOKAICHIYA, F.; SANTOS, I.A.; GUO, R.; BHALLA, A.S.Artigo IPEN-doc 22551 Pluronics F-127/L-81 binary hydrogels as drug-delivery systems: inlfuence of physicochemical aspects on release kinetics and cytotoxicity2014 - OSHIRO, ALISSON; SILVA, DEYSE C. da; MELLO, JOYCE C. de; MORAES, VIVIAN W.R. de; CAVALCANTI, LEIDE P.; FRANCO, MARGARETH K.K.D.; ALKSCHBIRS, MELISSA I.; FRACETO, LEONARDO F.; YOKAICHIYA, FABIANO; RODRIGUES, TIAGO; ARAUJO, DANIELE R. deArtigo IPEN-doc 22548 Cssub(2)NaAlsub(1-x)Crsub(x)Fsub(6): A family of compounds presenting magnetocaloric effect2014 - PEDRO, S.S.; TEDESCO, J.C.G.; YOKAICHIYA, F.; BRANDAO, P.; LANDSGESELL, S.; PIRES, M.J.M.; SOSMAN, L.P.; MANSANARES, A.M.; REIS, M.S.; BORDALLO, H.N.Artigo IPEN-doc 21336 Microfocus X-ray imaging of Brazil nuts for quality control2015 - FRANCO, MARGARETH K.K.D.; YOKAICHIYA, FABIANO; KARDJILOV, NIKOLAY; FERRAZ, ANTONIO C. de O.Artigo IPEN-doc 21331 Synchrotron powder diffraction study of cements pastes2015 - GARCEZ, E.O.; ALDRIGE, L.P.; RAVEN, M.; GATES, W.P.; COLLINS, F.; FRANCO, M.; YOKAICHIYA, F.Artigo IPEN-doc 21326 Assessment of cement durability in repository environment2015 - FERREIRA, EDUARDO G.A.; VICENTE, ROBERTO; TURRILLAS, XABIER; YOKAICHIYA, FABIANO; FRANCO, MARGARETH K.K.D.; MARTINEZ, LUIS G.; MARUMO, JULIO T.Artigo IPEN-doc 21224 Clonidine complexation with hydroxypropyl-beta-cyclodextrin: From physico-chemical characterization to in vivo adjuvant effect in local2016 - BRAGA, M.A.; MARTINI, M.F.; PICKHOLZ, M.; YOKAICHIYA, F.; FRANCO, MARGARETH K.K.D.; CABEÇA, L.F.; GUILHERME, V.A.; SILVA, C.M.G.; LIMIA, C.E.G.; PAULA, E. deArtigo IPEN-doc 21219 Assessing the pozzolanic activity of cements with added sugar cane straw ash by synchrotron X-ray diffraction and Rietveld analysis2015 - CALLIGARIS, GUILHERME A.; FRANCO, MARGARETH K.K.D.; ALDRIGE, LAURENCE P.; RODRIGUES, MICHELLE S.; BERALDO, ANTONIO L.; YOKAICHIYA, FABIANO; TURRILLAS, XAVIER; CARDOSO, LISANDRO P.Sugar and alcohol industries generate large amount of wastes that could produce ashes of great reactivity with pozzolan properties. The objective of this paper is to evaluate the pozzolanicity of Sugar Cane Straw Ashes (SCSA), thermal treated, at different curing times. Employing Synchrotron X-ray radiation for XRD measurements, scans from 10 to 110 (h 2h setup) allowed the quantification of several phases of the cement pasts through Rietveld analysis. The SCSA substitution of 20% (weight) in Ordinary Portland Cement (OPC) has improved the AFt (Ettringite) formation up to 47% for 90 days curing time. The Portlandite concentration analysis allowed concluding that this addition of SCSA in OPC has caused a delay in the cement setting time. Moreover, the behaviour of the C3S and Calcite contents in both OPC and OPC/SCSA samples were determined by refinement of the XRD pattern using the Rietveld method.