CLAUDIA REGINA CECCHI
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Artigo IPEN-doc 27539 Optimization of mouse growth hormone plasmid DNA electrotransfer into tibialis cranialis muscle of "little" mice2020 - LIMA, ELIANA R.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; JESUS, GUSTAVO P.P. de; GOMES, ALISSANDRA M.; ZACARIAS, ENIO A.; BARTOLINI, PAOLO; PERONI, CIBELE N.Previous non-viral gene therapy was directed towards two animal models of dwarfism: Immunodeficient (lit/scid) and immunocompetent (lit/lit) dwarf mice. The former, based on hGH DNA administration into muscle, performed better, while the latter, a homologous model based on mGH DNA, was less efficient, though recommended as useful for pre-clinical assays. We have now improved the growth parameters aiming at a complete recovery of the lit/lit phenotype. Electrotransfer was based on three pulses of 375 V/cm of 25 ms each, after mGH-DNA administration into two sites of each non-exposed tibialis cranialis muscle. A 36-day bioassay, performed using 60-day old lit/lit mice, provided the highest GH circulatory levels we have ever obtained for GH non-viral gene therapy: 14.7 ± 3.7 ng mGH/mL. These levels, at the end of the experiment, were 8.5 ± 2.3 ng/mL, i.e., significantly higher than those of the positive control (4.5 ± 1.5 ng/mL). The catch-up growth reached 40.9% for body weight, 38.2% for body length and 82.6%–76.9% for femur length. The catch-up in terms of the mIGF-1 levels remained low, increasing from the previous value of 5.9% to the actual 8.5%. Although a complete phenotypic recovery was not obtained, it should be possible starting with much younger animals and/or increasing the number of injection sites.Resumo IPEN-doc 22302 Efficient and non-invasive plasmid DNA administration into the tibialis cranialis muscle of dwarf mice2014 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; LIMA, ELIANA R.; AZEVEDO, MAYARA L. de; AAGAARD, LARS; BARTOLINI, PAOLO; PERONI, CIBELE N.Resumo IPEN-doc 21241 Early treatment for growth hormone deficiency based on naked DNA administration in dwart mice allows efficient catch-up growth2015 - HIGUTI, ELIZA; CECCHI, CLAUDIA R.; LIMA, ELIANA R.; AAGAARD, LARS; JENSEN, THOMAS G.; BARTOLINI, PAOLO; PERONI, CIBELE N.Capítulo IPEN-doc 17391 Different ex vivo and direct in vivo DNA administration strategies for growth hormone gene therapy in dwarf animals2011 - PERONI, CIBELE N.; OLIVEIRA, NELIO A. de J.; CECCHI, CLAUDIA R.; HIGUTI, ELIZA; BARTOLINI, PAOLOResumo IPEN-doc 17526 Growth parameters after intramuscular hGH plasmid administration compared to recombinant hGH injections in LIT/SCID mice2011 - HIGUTI, E.; CECCHI, C.R.; OLIVEIRA, N.A.J.; SILVA, J.T.; JORGE, A.A.L.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 17367 Non-viral gene transfer to skin, muscle and liver for expression of growth homone2011 - CECCHI, CLAUDIA R.; DAGUACS HANSEN, FREDERIK; AGAARD, LARS; GISSEL, HANNE; GOTHELF, ANITA; JAKOBSEN, MARIA; HANSEN, BIRGIT H.; FRYSTYK, JAN; OLIVEIRA, NELIO A.J.; HIGUTI, ELIZA; BARTOLINI, PAOLO; PERONI, CIBELE N.; JENSEN, THOMAS G.Resumo IPEN-doc 16659 Electrotransfer of naked hGH cDNA muscle of lit/scid mouse is more efficient than genomic DNA2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; MORISCOT, A.S.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16651 Sustained hGH expression after electrotransfer of naked DNA into DWARF 'little!' mouse skeletal muscle2009 - OLIVEIRA, N.A.J.; CECCHI, C.R.; HIGUTI, E.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 16421 Growth parameters after intramuscular hGH plasmid administration compared to recombinant hGH injections in lit/scid mice2011 - HIGUTI, E.; CECCHI, C.R.; OLIVEIRA, N.A.J.; SILVA, J.T.; JORGE, A.A.L.; BARTOLINI, P.; PERONI, C.N.Resumo IPEN-doc 15598 Highly significant body weight increase of Lit/Scid mice after electrotransfer of hGH-coding plasmid DNA2010 - CECCHI, C.R.; OLIVEIRA, N.A.J.; HIGUTI, E.; JENSEN, M.; BARTOLINI, P.; PERONI, C.N.