GABRIELA ORTEGA COELHO THOMAZI
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Artigo IPEN-doc 27224 Paratrygon aiereba irradiated anti-mucus serum reduce edematogenic activity induced in experimental model2020 - THOMAZI, GABRIELA O.C.; COSTA, ANDREA da; RODRIGUES, JAQUELINE P.; ALVES, GLAUCIE J.; PREZOTTO NETO, JOSE P.; TURIBIO, THOMPSON de O.; ROCHA, ANDRE M.; AIRES, RAQUEL da S.; SEIBERT, CARLA S.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. de; NASCIMENTO, NANCI doAccidents by freshwater stingrays are common in northern Brazil, there is no specific therapy for high morbidity and local tissue destruction. The irradiation of venoms and toxins by ionizing radiation has been used to produce appropriate immunogens for the production of antisera. We planned to study the efficacy of stinging mucus irradiation in the production of antisera, with serum neutralization assays of edematogenic activity and quantification of cytokines performed in animal models of immunization with native and irradiated mucus of Paratrygon aiereba, a large freshwater stingray. Antiserum potency and its cross-reactivity with mucus from other freshwater stingrays were detected by ELISA. Immunization models demonstrated the ability to stimulate a strong humoral response with elevated levels of serum IgG detectable by ELISA, and both native and irradiated mucus were immunogenic and capable of recognizing mucus proteins from other freshwater neotropical stingrays. Mucus P. aiereba causes cellular and humoral adaptive immune responses in cells of immunized mice producing antibodies and cytokines such as TNF-α, IL-6 and IL-17. Rabbit antisera immunized with mucus from P. aiereba irradiated at 2 kGy showed a significant reduction of mucus-induced edematogenic activity in mice. Our data suggest that the use of antisera against freshwater stingray mucus show the possibility of specific therapy for these accidents.Artigo IPEN-doc 24156 Previous 60-CO radiation from Paratrygon aiereba mucus induces the production of highly responsive antibodies and a better immune response in mice2017 - THOMAZI, GABRIELA O.C.; COSTA, ANDREA da; RODRIGUES, JAQUELINE P.; ALVES, GLAUCIE J.; TURIBIO, THOMPSON de O.; ROCHA, ANDRE M.; AIRES, RAQUEL da S.; JACOME, LARISSA B.S.; SEIBERT, CARLA S.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J.; ANDRADE JUNIOR, HEITOR F. deWounds from stinging freshwater stingrays are painful, difficult to heal and cause extensive necrosis and systemic phenomena. The treatment is symptomatic, of low efficiency and there is no therapy, which causes more suffering to the injured. This study aimed to evaluate the immune response induced by the native or irradiated by 60-Co gamma from Paratrygon aiereba mucus. IPEN’s Committee on Ethics in the Use of Animals (n.º126/2013) and lanes captured under license from the Chico Mendes Institute for Biodiversity Conservation (n.º6781-1/2014) approved this research. For the assays, sera from Swiss mice previously immunized against native or irradiated mucus were used. The proliferation of splenic B cells in response to mucus was evaluated by the In Vitro Induced Antibody Production method and serum and splenic cytokines were also quantified. Our data demonstrate that the irradiated mucus of P. aiereba induces greater production of antibodies and more immunological memory in the mice. Spleen cells from animals immunized against irradiated mucus produced IFN-γ, TNF-α and IL-10, and serum TNF-α (immunized group against irradiated mucus) and IL-6 and IL-17 (immunized group against native mucus). The results corroborate the use of ionizing radiation, with production of highly responsive antibodies and better immune response, besides proving that Paratrygon aiereba mucus is capable of stimulating cellular and humoral adaptive immune response, contributing to the continuity of associated investigations.Artigo IPEN-doc 21107 Comparative nephrotoxicity of native or Co-60 gamma rays irradiated crotoxin in mice2015 - ROCHA, ANDRE M.; ROCHA, JOSEFA M.N.; MAGALHAES FILHO, ASTERIO S.; ALVES, GLAUCIE J.; AIRES, RAQUEL da S.; TURIBIO, THOMPSON O.; THOMAZI, GABRIELA O.C.; SPENCER, PATRICK J.; NASCIMENTO, NANCI doArtigo IPEN-doc 21030 Humoral immune response against native or sup(60)Co irradiated venm and mucus from stingray Paratrygon aiereba2015 - THOMAZI, GABRIELA O.C.; ALVES, GLAUCIE J.; AIRES, RAQUEL da S.; TURBIO, THOMPSON de O.; ROCHA, ANDRE M.; SEIBERT, CARLA S.; SPENCER, PATRICK J.; NASCIMENTO, NANCI do