DÉCIO DOS SANTOS PINTO JUNIOR
3 resultados
Resultados de Busca
Agora exibindo 1 - 3 de 3
Artigo IPEN-doc 19178 Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQo-induced potentially malignant oral lesions2013 - BARCESSAT, ANA R.; HUANG, ISAAC; ROSIN, FLAVIA P.; PINTO JUNIOR, DECIO dos S.; ZEZELL, DENISE M.Fractionation can improve photodynamic therapy (PDT) efficacy for potentially malignant oral lesion treatment. The aim of this study was to demonstrate the apoptosis/proliferation index of oral keratinocytes after two sessions of topical 5-ALA-mediated PDT in 4-Nitroquinoline-1-oxide-induced potentially malignant oral lesion, and to suggest the ideal interval between PDT sessions. Immuno-histochemical tests for proliferating cell nuclear antigen and caspase-3, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay were performed at 6 h, 24 h, 48 h, and 72 h time intervals after PDT. The number of positive cells showing caspase-3 expression was significantly higher, mainly at 6 h after PDT. In the first cycle of PDT, the highest frequency of positive cells for TUNEL was found at 24 h. At 72 h after PDT, proliferating cell nuclear antigen positive cells increased significantly, indicating that there was an epithelial response in direction towards DNA repair and cell proliferation at this time. Because cell proliferation increases and cell death index decreases at 72 h after PDT, it is recommended that the interval between the PDT sessions must not be longer than 2 days up to total lesion remission.Artigo IPEN-doc 17729 Evaluation of microRNA expression in head and neck squamous cell carcinoma cell lines and in primary culture of oral keratinocytes2011 - ANDREGHETTO, FLAVIA M.; KLINGBEIL, MARIA F.G.; SOARES, RENATA M.; SITNIK, ROBERTA; PINTO JUNIOR, DECIO dos S.; MATHOR, MONICA B.; NUNES, FABIO D.; SEVERINO, PATRICIAArtigo IPEN-doc 15789 Is it safe to utilize in vitro reconstituted human oral epithelium? An oncogenetic pathway study2012 - KLINGBEIL, MARIA F.G.; MATHOR, MONICA B.; GIUDICE, FERNANDA S.; YOSHITO, DANIELE; PINTO JUNIOR, DECIO dos S.