DALTON GIOVANNI NOGUEIRA DA SILVA

DALTON GIOVANNI NOGUEIRA DA SILVA

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X- ray experimental set-up for in-vitro nail test
2023 - ZAMBONI, C.B.; GIOVANNI, D.N.S.; ALMEIDA, M.R.; TODO, A.S.; SABARA, F.; RODRIGUES JUNIOR, O.
In this investigation, the elements Ca and S were evaluated in a single nail clipping using compact and portable X-ray spectrometer model X-123 SDD with Ag target. Experimental conditions for current, voltage and excitation time were investigated. These analyses can be useful for a variety of applications, including nutritional and medical diagnosis, such as, the evaluation of bone dysfunctions by measurement of Ca (bone decalcification) and S for nutrition evaluations (collagen production). This analysis offers some benefits comparatively to blood and serum analyses, such as, non-invasive collection, fast analyses (minutes) and low cost. In addition, nail clippings are simple to obtain, easy to store, and easy to transport. This pilot study show a positive expectation for clinical application using in-vitro nail test.
Uso de mini-espectrômetro de fluorescência de raios X como alternativa para a prática clínica de dialisados
2023 - SOUZA, CATARINA da S.; ZAMBONI, CIBELE B.; MOURA, LARISSA A.S.; METAIRON, SABRINA; SILVA, DALTON G.N. da
Neste estudo, o uso de equipamento compacto e portátil, baseado na tecnologia de Fluorescência de Raios X, foi empregado para execução de análises bioquímicas (dosagens de íons) em microamostras de fluidos corpóreos depositadas em papel. Foi realizada a análise simultânea de íons de relevância clínica e nutricional (Ca, Cl, Fe, K, P e S), em sangue total de pacientes com insuficiência renal crônica (IRC) submetidos a tratamento dialítico. Durante a investigação as concentrações obtidas em sangue total levaram a resultados que corroboram com o quadro clínico obtido pelas análises convencionais. Com os dados do presente foi possível elaborar uma discussão sobre a viabilidade de uso deste equipamento para a realização desses exames bioquímicos em Unidades Básicas de Saúde Fluviais (UBSF), gerando melhorias na qualidade de vida de populações ribeirinhas, bem como no âmbito econômico em função de sua aplicabilidade com menor custo. Descritores: Íons, Sangue, Análise Clínica.
Comparação de desempenho do espectrômetro portátil de fluorescência de raios-X utilizando alvos de Au e Ag para análise de amostras na área da saúde
2022 - TASSO, ORION G.; ZAMBONI, CIBELE B.; SILVA, ANA C.K. de S.; MEDEIROS, JOSE A.G. de; SILVA, DALTON G.N. da
Este trabalho teve por objetivo avaliar o desempenho do espectrômetro portátil e compacto de Fluorescência de Raios-X (pFRX), para dosagem de íons em amostras biológicas de interesse na área da saúde (prioritariamente fluidos corpóreos como soro e urina), utilizando alvos de Prata (Ag) e Ouro (Au). O foco das comparações foi direcionado às análises quantitativas de elementos de relevância clínica e nutricional (prioritariamente a dosagem de Ca, Cl, Fe, K, P e S). Para este estudo foram avaliados (para cada alvo) os parâmetros: linearidade, reprodutibilidade, acurácia, sensibilidade e limite de detecção. Essas investigações permitiram a realização de um estudo comparativo entre os alvos, com a finalidade de fornecer subsídios que permitem a escolha adequada do alvo (Ag e/ou Au), agilizando as medidas além de fornecer resultados mais precisos. Os resultados de FRX em soro e urina formam comparados com as dosagens obtidas pela técnica de Análise por Ativação com Nêutrons e apresentaram boa concordância.
Non-destructive analysis in the study of historical photographs by pXRF and ATR-FTIR spectroscopies
2021 - ZAMBONI, CIBELE B.; REDÍGOLO, MARCELO M.; MIURA, VINICIUS T.; COSTA, ISOLDA; NAGAI, MARIA L.E.; SALVADOR, PABLO A.V.; SILVA, DALTON G.N. da
Material characterization is essential to the provenance of graphic arts. Non-destructive analytical techniques are increasingly required in the authentication process of cultural heritage. This work presents a suite of portable, non-destructive, and complementary analytical techniques, energy dispersive x-ray fluorescence (EDXRF), Fourier transform infrared (FTIR) spectroscopies, and brightfield microscopy, applied to the analysis of historical photographs depicting São Paulo city architecture, whose registration date and process of fabrication are unknown. The EDXRF analysis emphasizes the use of typical POP (printing-out paper) photograph with baryta (BaSO4) coated paper substrate while the FTIR and microscopy analyses confirm the presence of collodion and a gelatin-based baryta layer. This photographic process was widely employed by professional photographers from 1889 to 1930, when it was gradually abandoned in commercial use. This time interval (1889–1930) is consistent with the information surveyed on the photographic collection. In conclusion, employing complementary techniques (elemental and molecular spectroscopies and image magnification) is essential in identifying the manufacturing materials of cultural heritage material, which is the basis of contemporary authentication procedures. These data provide to curators and historians fundamental information for cataloging, adding subsidies for the correct storage and preservation (“heritage appreciation”). Still, for professional photographers, they present information on the manufacturing processes of historical photographs. The data from the present study also emphasize its perspective of use in graphic arts to aid connoisseurship in identifying forgeries during provenance and authentication studies.
Sodium analysis in sweeteners by neutron activation analyses technique
2019 - MEDEIROS, I.M.M.A.; ZAMBONI, C.B.; GIOVANNI, D.N.S.; MEDEIROS, J.A.G.
Evaluation of Sodium in sweeteners is important in nutritional investigations and for consumers: excessive sodium consumption is one of the major risk factors, responsible for hypertension and cardiovascular diseases. In this study, twelve brands acquired in markets of São Paulo city were analysed by Instrumental Neutron Activation Analyses technique (INAA). The aim was to verify compliance with ANVISA recommendation in relation to sodium level. The results were compared with the amounts recommended and with the tolerable intake limit (< 0.4 g/kg). The sodium concentration in sweeteners samples showed low content for most of the brands. The results shown that for the general population, it is not risk.
Ions concentration in blood samples of SJL/J dystrophic mice strains using X-ray fluorescence spectrometry
2019 - GIOVANNI, D.N.S.; ALMEIDA, M.R.; ZAMBONI, C.B.; METAIRON, S.; BALDUINO, K.N.; SUZUKI, M.F.; BUENO JUNIOR, C.R.
Star This study proposes an investigation of ions in whole blood of the dystrophic animal model SJL/J (mice strain with dysferlin protein deficiency) and in the control group (C57BL/6J) using the Energy Dispersive X-ray Fluorescence (EDXRF) spectrometry technique. The comparison between control and dystrophic mice results shown an increase in blood for P, S, K and Fe (p < 0.05) while a decrease in Ca (p < 0.05). This elemental analysis will contribute to evaluate the best diagnostic, care and treatment procedures, for the Progressive Muscular Dystrophy.
Sulfur status in judo athletes by XRF
2019 - ALMEIDA, M.R.; ZAMBONI, C.B.; GIOVANNI, D.N.S.; AZEVEDO, M.R.A.
The human biomonitoring, measurement of chemical and/or their metabolites in human fluids, is an important tool for assessing the health condition of subjects, included athletes. In this study, sulfur levels were investigated in blood of judo athletes using Energy Dispersive X-ray Fluorescence (EDXRF) technique. Twenty athletes participated of this study. Two groups of athletes were selected: judo with a balanced diet with multivitamin/mineral supplements consumption and judo with diet not controlled. These data were compared with the control group (subjects of the same age but not involved with physical activities). There was a significant increase of S levels in athletes with diet not controlled. These data can be useful to a well-planned nutritional proposition that can contribute to better performance of athletes.
Multielemental analyses of Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar using EDXRF and INAA techniques
2019 - ZAMBONI, C.B.; GIOVANNI, D.N.S.; SALVADOR, V.L.R.; SATO, I.M.; MENDONÇA, R.Z.; WEN, F.H.; SIMONS, M.S.
In this study, multielemental analysis of Lonomia obliqua (Lepidoptera, Saturniidae) caterpillar was performed using energy dispersive X‐ray fluorescence and instrumental neutron activation analysis techniques. This caterpillar is poisonous and has the ability to cause fatal hemorrhagic effects in humans after contact. The need of this study is related to morphological changes (mainly size and color) observed in some caterpillars used for preparation of antilonomic serum (antivenom). The samples were classified as healthy (caterpillars of control) and unhealthy (caterpillars visibly modified). The XRF measurements were performed in an energy dispersive X‐ray fluorescence spectrometer and the instrumental neutron activation analysis using the IEAR1 nuclear reactor at IPEN. The results show significant differences for several elements (mainly, P, Cl, Ca, Ti, Mn, Fe, Ni, and Zn) in unhealthy caterpillars that can affect the development of this species as well as the quality and yield of the antivenom. Furthermore, its elemental characterization contributes for the understanding the potential pharmacological (procoagulant and antithrombotic) in the prevention of life‐threatening blood clots.
Iron determination in whole blood samples of dystrophic mouse strains using X-Ray fluorescence spectrometry
2017 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; GIOVANNI, DALTON N. da S.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.
Several diseases can be monitored by assessing the variation of specific ion concentration in body fluids. This study proposes an alternative analysis, based on the X-ray fluorescence spectrometry technique, for the investigation of iron (Fe concentration determination) in whole blood of the dystrophic animal models. We performed blood analyses from three different strains with Muscular Dystrophy (Dmdmdx/ J, SJL/J and A/J) and from healthy (C57BL/6J) mice strain adopted as the control group. The results emphasize that iron levels in whole blood male samples of SJL/J and A/J are significantly higher. Related to the gender for Dmdmdx/J and SJL/J mouse strains the comparison emphasizes that iron level increase for males. The study of Fe behavior in whole blood mice samples with muscular dystrophy contributes to its diagnosis, providing a better understanding of this disease.