DALTON GIOVANNI NOGUEIRA DA SILVA

DALTON GIOVANNI NOGUEIRA DA SILVA

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Uso de mini-espectrômetro de fluorescência de raios X como alternativa para a prática clínica de dialisados
2023 - SOUZA, CATARINA da S.; ZAMBONI, CIBELE B.; MOURA, LARISSA A.S.; METAIRON, SABRINA; SILVA, DALTON G.N. da
Neste estudo, o uso de equipamento compacto e portátil, baseado na tecnologia de Fluorescência de Raios X, foi empregado para execução de análises bioquímicas (dosagens de íons) em microamostras de fluidos corpóreos depositadas em papel. Foi realizada a análise simultânea de íons de relevância clínica e nutricional (Ca, Cl, Fe, K, P e S), em sangue total de pacientes com insuficiência renal crônica (IRC) submetidos a tratamento dialítico. Durante a investigação as concentrações obtidas em sangue total levaram a resultados que corroboram com o quadro clínico obtido pelas análises convencionais. Com os dados do presente foi possível elaborar uma discussão sobre a viabilidade de uso deste equipamento para a realização desses exames bioquímicos em Unidades Básicas de Saúde Fluviais (UBSF), gerando melhorias na qualidade de vida de populações ribeirinhas, bem como no âmbito econômico em função de sua aplicabilidade com menor custo. Descritores: Íons, Sangue, Análise Clínica.
Quantitative analysis of light elements using compact XRF spectrometers
2021 - ZAMBONI, CIBELE B.; SILVA, DALTON G.N. da; TASSO, ORION G.; MEDEIROS, JOSE A.G. de; AZEVEDO, MARIA R.A. de
The performance of two compact X-Ray Fluorescence (FRX) spectrometers consisting of Ag and Au X-ray mini-tubes associated with a Si Drift detector was checked for clinical finality. Using the Energy Dispersive X-Ray Fluorescence technique (FRXDE) specific light elements of clinical relevance (Ca, Cl, K and Fe) were determined in human whole blood samples without chemical digestion using direct and non-destructive analysis, in air atmosphere. A comparative study between Ag and Au targets data was carried out and the results are in good agreement.
Ions concentration in blood samples of SJL/J dystrophic mice strains using X-ray fluorescence spectrometry
2019 - GIOVANNI, D.N.S.; ALMEIDA, M.R.; ZAMBONI, C.B.; METAIRON, S.; BALDUINO, K.N.; SUZUKI, M.F.; BUENO JUNIOR, C.R.
Star This study proposes an investigation of ions in whole blood of the dystrophic animal model SJL/J (mice strain with dysferlin protein deficiency) and in the control group (C57BL/6J) using the Energy Dispersive X-ray Fluorescence (EDXRF) spectrometry technique. The comparison between control and dystrophic mice results shown an increase in blood for P, S, K and Fe (p < 0.05) while a decrease in Ca (p < 0.05). This elemental analysis will contribute to evaluate the best diagnostic, care and treatment procedures, for the Progressive Muscular Dystrophy.
Sulfur status in judo athletes by XRF
2019 - ALMEIDA, M.R.; ZAMBONI, C.B.; GIOVANNI, D.N.S.; AZEVEDO, M.R.A.
The human biomonitoring, measurement of chemical and/or their metabolites in human fluids, is an important tool for assessing the health condition of subjects, included athletes. In this study, sulfur levels were investigated in blood of judo athletes using Energy Dispersive X-ray Fluorescence (EDXRF) technique. Twenty athletes participated of this study. Two groups of athletes were selected: judo with a balanced diet with multivitamin/mineral supplements consumption and judo with diet not controlled. These data were compared with the control group (subjects of the same age but not involved with physical activities). There was a significant increase of S levels in athletes with diet not controlled. These data can be useful to a well-planned nutritional proposition that can contribute to better performance of athletes.
Evaluation of iron in blood of athletes by the EDXRF technique
2019 - ALMEIDA, MATEUS R. de; ZAMBONI, CIBELE B.; SILVA, DALTON G.N. da; AZEVEDO, MARIA R.
The aim of this study was evaluate Fe in blood of athletes from different modalities (judo athletes, cyclist and long distance runners) by X-ray Fluorescence methodology using portable equipment, as an alternative for clinical practice. The study showed the practicality and efficacy of using this methodology for successive clinical evaluations, during the preparation period of competitions, providing data that help in the elaboration of a balanced diet, as well as contribute to the proposal of new clinical evaluation protocols. In addition, the results emphasize the need to adopt differentiated diets for adequate iron intake as a function of sports activity.
Uso de mini-espectrômetro de fluorescência de raios-x como alternativa para prática clínica de dialisados
2019 - SOUZA, CATARINA S.; ZAMBONI, CIBELE B.; SILVA, DALTON G.N. da; METAIRON, SABRINA
O objetivo do presente estudo foi avaliar a performance da técnica de Fluorescência de Raios-X por Dispersão de Energia (FRX-DE) para análise de íons, de relevância clínica (Ca, Cl, K, Fe), em sangue total de pacientes com insuficiência renal crônica (IRC) submetidos a tratamento dialítico. Com os dados do presente foi possível elaborar uma discussão sobre as vantagens e limitações do uso deste procedimento para a realização desses exames bioquímicos em Centros de Hemólise. Durante a investigação as concentrações obtidas para Ca, Cl, K e Fe levaram a resultados que corroboram com o quadro clínico obtido pelas análises convencionais.
Correlation analysis of inorganic elements in whole blood of GRMD dogs using INAA
2019 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; SILVA, DALTON G.N. da
Instrumental neutron activation analysis technique (INAA) has been used to determine Br, Ca, Cl, Cr, Fe, K, Mg, Na, P and S concentrations in whole blood of GRMD dogs’ samples from control, carrier and affected dogs (treated and untreated). The GRMD dogs are relevant as an experimental model for Duchenne Muscular Dystrophy (DMD), which is the most severe and prevalent type of muscular dystrophy. Muscle weakness, premature death and instability of the membrane that involves the muscle fibers - causing functional/structural abnormalities and cell death - are main characteristics of this genetic disease. To show in more details the alterations that this disease may cause in whole blood, correlations matrixes were generated for control, carried and affected groups permitting a comparison between them. The results emphasize physiologic differences for K and Mg and suggest that Br results are altered, emphasizing a constant monitoring need. Other than that, these results may help the researchers to evaluate the efficiency of new treatments and to compare the advantages of different treatment approaches before performing tests in patients with muscular dystrophy.
Progress and trends for radiation applications in clinical practice
2019 - ZAMBONI, CIBELE B.; METAIRON, SABRINA; MEDEIROS, JOSE A.G. de; SILVA, DALTON G.N. da; TASSO, ORION G.
In this study, we intend to optimize the use of equipment based on the X-Ray Fluorescence technology to perform ions dosage in body fluids. This procedure can be an efficient alternative for clinical diagnosis, mainly in underserved regions of Brazil with the deficit of medical care in the hospitals. In addition, this procedure has potential use when the biological material is scarce, case of the pediatric practice in newborns and premature infants (blood collection is the main cause of transfusions) as well as in Hemodialysis units which requires several biochemical tests (before and after dialysis).
Iron determination in whole blood samples of dystrophic mouse strains using X-Ray fluorescence spectrometry
2017 - METAIRON, SABRINA; ZAMBONI, CIBELE B.; GIOVANNI, DALTON N. da S.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.
Several diseases can be monitored by assessing the variation of specific ion concentration in body fluids. This study proposes an alternative analysis, based on the X-ray fluorescence spectrometry technique, for the investigation of iron (Fe concentration determination) in whole blood of the dystrophic animal models. We performed blood analyses from three different strains with Muscular Dystrophy (Dmdmdx/ J, SJL/J and A/J) and from healthy (C57BL/6J) mice strain adopted as the control group. The results emphasize that iron levels in whole blood male samples of SJL/J and A/J are significantly higher. Related to the gender for Dmdmdx/J and SJL/J mouse strains the comparison emphasizes that iron level increase for males. The study of Fe behavior in whole blood mice samples with muscular dystrophy contributes to its diagnosis, providing a better understanding of this disease.
Iron determination in whole blood samples of dystrophic mice strains using X-ray fluorescence spectrometry
2017 - SILVA, DALTON G.N. da; METAIRON, SABRINA; ZAMBONI, CIBELE B.; SUZUKI, MIRIAM F.; BUENO JUNIOR, CARLOS R.; RIZZUTTO, MARCIA de A.
The use of alternative analytical techniques to investigate specific electrolytes in body fluids has increased in past years with the goal of adding advantages and simplifications, compared to the procedures used in conventional clinical practice. Specifically, in this study, a portable X-ray spectrometer (PXRFS) from Amptek (model X-123 SDD) with Ag X-ray mini-tube has been employed for the whole blood iron measurements in mice species with Muscular Dystrophy (Dmdmdx/J, SJL/J and A/J). The term whole blood refers to solid (cells) and liquid (plasma) components in blood. Usually the conventional procedures for clinical tests are performed in serum using samples of 0.5 to 1.0 mL. However, when the biological material is scarce (case of small size animal model, i.e. mice), the possibility to use whole blood became a fascinating alternative for clinical practice: the Energy Dispersive X-Ray Fluorescence (EDFRX) analyses, using PXRFS, can be done using 50 μL (the total body blood in mice is around 1.0 mL). In this study, we investigated whole blood samples of dystrophic mice strains, a genetic disease that cannot be reversed. In this investigation, twenty samples were investigated. The blood samples were obtained from Jackson Laboratory (Maine, USA) and further inbred at IPEN. The whole blood was collected by the retro–orbital venous plexus and immediately transferred to filter paper (Whatman n41), prior to its coagulation. No anticoagulants or reagents have been used to prevent interference on the results. This procedure simplify the preparation and conservation of biological samples: it is not a destructive procedure and the sample does not need refrigeration. The collection was performing according to the rules approved by Animal Research Ethics Committee (087/99). A comparative study between EDXFX and INAA (Instrumental Neutron Activation Analysis) data was also carried out and the results are in good agreement. This alternative procedure was capable, in a few minutes, to determine whole blood Fe concentrations higher than 6.5 mgL-1, without chemical digestion using direct and non-destructive analysis. The study of Fe behavior in whole blood samples of mice with muscular dystrophy contributes to its diagnosis.