LARISSA MIRANDA PEREIRA
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Artigo IPEN-doc 24238 Structural characterization and enzymatic activity of the recombinant Ala959 to Ser1066 region of human ace2017 - ELIASA, CAROLINE C.; PEREIRA, LARISSA M.; ARAGAO, DANIELLE S.; CASARINI, DULCE E.; AFFONSO, REGINAAngiotensin-converting enzyme catalyzes the conversion of angiotensin I to the vasoconstrictor angiotensin II and the hydrolysis of bradykinin (BK). Human somatic angiotensin-converting enzyme has two homologous domains (N and C) that share 60% identity. Although these two regions have high homology, the catalytic site of the C-domain exhibits three-fold greater activity than the N-domain in the hydrolysis of angiotensin I in vivo. The present study aimed to obtain the Ala959 to Ser1066 catalytic region of the C-domain of angiotensin-converting enzyme in a structural conformation that resembles its native form. We amplified the 324-bp sequence corresponding to the catalytic site of C-domain of angiotensin-converting enzyme and cloned this sequence into a pET28 vector. The catalytic site of C-domain of angiotensin-converting enzyme peptide was expressed in a bacterial system, and its purification was performed in one step using a His-tag affinity column. Structural analysis by circular dichroism and fluorescence confirmed that the purified protein is correctly folded, and catalytic site of C-domain of angiotensin-converting enzyme possesses enzymatic activity and is inhibited by lisinopril. This peptide can be used to test new inhibitors and C-domain of angiotensin-converting enzyme substrates because this peptide is easy to produce and this has proven efficient link with these molecules.Resumo IPEN-doc 16192 Expression and characterization of the human L10(QM) protein in E. coli2010 - PEREIRA, L.M.Resumo IPEN-doc 18649 Cloning, expression, purification and structural evaluation of the jun oncoprotein2012 - SILVA, S.F.; PEREIRA, L.M.; SARAIVA, K.W.; CHARRO, S.G.; AFFONSO, R.Resumo IPEN-doc 18648 Structural analysis of the human RPL10 protein expressed in bacterial culture in soluble form2012 - PEREIRA, L.M.; SILVA, F.S.; SARAIVA, K.W.; CHARRO, S.G.; SILVA, I.D.C.G.; AFFONSO, R.Resumo IPEN-doc 19732 Characterization of recombinant full-length human protein RPL-102012 - PEREIRA, L.M.; SILVA, S.F.; SARAIVA, K.W.; ELIAS, C.C.; ALVES, M.M.; PEREIRA, V.S.; AFFONSO, R.