GUSTAVO BERNARDES FANARO
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Resumo IPEN-doc 17434 Advances of E-beam processing for food preservation in Brazil2009 - VILLAVICENCIO, A.L.C.H.; ARAUJO, M.M.; SILVA, P.V.; FANARO, G.B.Artigo IPEN-doc 15260 Effects of gamma radiation on sensorial properties in black tea2009 - SILVEIRA, ANA P.M.; FANARO, GUSTAVO B.; COSTA, HELBERT S.F.; SILVA, PRISCILA V.; SANTILLO, AMANDA G.; VILLAVICENCIO, ANNA L.C.H.Artigo IPEN-doc 15147 Gamma irradiation as a quarantine treatment against eggs of citrus black fly (Aleurocanthus Woglumi Ashby)2009 - VILLAVICENCIO, ANNA L.C.H.; ARAUJO, MICHEL M.; FANARO, GUSTAVO B.; COSTA, HELBERT S.F.; SILVA, PRISCILA P.V.; ARHUR, VALTER; FARIA, JOSE T.Artigo IPEN-doc 12686 Effects of electron beam treatment in soybean grains artificially inoculated by Phakopsora pachyrhizi2007 - FANARO, G.B.; SILVA, P.V.; NUNES, T.C.F.; ROGOVSCHI, V.D.; AQUINO, S.; VILLAVICENCIO, A.L.C.H.Artigo IPEN-doc 12745 Detection of Phakopsora pachyrhizi by polymerase chain reaction (PCR) and use of germination test and DNA comet assay afther e-beam processing in soybean2007 - VILLAVICENCIO, A.L.C.H.; FANARO, G.B.; ARAUJO, M.M.; AQUINO, S.; SILVA, P.V.; MANCINI FILHO, J.Soybean harvest is the main agribusiness in Brazil, which is the second largest exporter in the world and has a revenue of billions of dollars. Asian dust is caused by the fungus Phakopsora pachyrhizi and its dissemination is difficult to control, since it occurs through wind dispersion. Actually P. pachyrhizi is found in different parts of the world. Electron beam treatment could be an alternative process to minimize these losses, especially for the grains exportation industry. Besides the possibility of being disconnected when not in use, this source does not need to be reloaded, is easily available and, streamlines the process and reduces logistics costs. The present work aims to identify, by the polymerase chain reaction technique (PCR), the P. pachyrhizi fungus presence in the irradiated soybeans and the possibility to use radiation treatment as a sanitary alternative. Doses 0, 1.0, 2.0, 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 kGy (IPEN-CNEN/SP Electron Accelerator) were applied and two fast-screening methods were performed: DNA comet assay (for the detection of DNA damage) and germination test (for the measurement of roots inhibition). These tests are very easy to carry out and measure damage response depending on radiation dose.