GUSTAVO BERNARDES FANARO

GUSTAVO BERNARDES FANARO

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Deterccao da Phakopsora pachyrhizi por reacao em cadeia pela polimerase (PCR) depos do tratamento dos graos de soja por feixes de eletrons
2005 - FANARO, G.B.; AQUINO, S.; GUEDES, R.L.; CREDE, R.G.; SABUNDJIAN, I.T.; RUIZ, M.O.; VILLAVICENCIO, A.L.C.H.
Graos de soja inoculados artificialmente com o fungo causados da ferrugem da soja (Phakopsora pachyrhizi) processados por feixe eletrons
2005 - SABUNDJIAN, I.T.; AQUINO, S.; GUEDES, R.L.; FANARO, G.B.; CREDE, R.G.; VILLAVICENCIO, A.L.C.H.
Evaluation of fungal contamination in irradiated phytoterapic
2005 - AQUINO, S.; DARTORA, M.M.P.A.; GONCALVES, E.; SABUNDJIAN, G.; GUEDES, R.L.; FANARO, G.B.; CREDE, R.G.; CORREA, B.; VILLAVICENCIO, A.L.C.H.
Efeitos da radiacao gama em alimentos miniminamente processados contaminados artificialmente com Escherichia coli
2005 - GUEDES, R.L.; CREDE, R.G.; SABUNDJIAN, I.T.; AQUINO, S.; RUIZ, M.O.; FANARO, G.B.; VILLAVICENCIO, A.L.C.H.
Effects of electron beam treatment in soybean grains artificially inoculated by Phakopsora pachyrhizi
2007 - FANARO, G.B.; SILVA, P.V.; NUNES, T.C.F.; ROGOVSCHI, V.D.; AQUINO, S.; VILLAVICENCIO, A.L.C.H.
Irradiation as a decontamination processing for rice paper sheet
2007 - ARAUJO, MICHEL M.; THOMAZ, FERNANDA S.; FANARO, GUSTAVO B.; DUARTE, RENATO C.; AQUINO, SIMONE; CORREA, BENEDITO; VILLAVICENCIO, ANNA L.C.H.
Starch is one of the most important plant products to man. The major sources of this compound for man's use are the cereals, but roots and tubers are also important. The starch industry comes in recent years growing and perfecting it self, leading to the necessity products with specific characteristics that take care the requirements of the market, it makes possible through processing raw material, still seldom explored. Rice paper sheet is an edible product derived from potatoes and rice, being commonly used to cover cakes, pies, and sweets in confectioner’s shop. A microbiological control is necessary to give a high quality and to guarantee the security of this food. Irradiation would be a safe alternative as a decontamination method without adverse effects on the physical properties in the final products. The aim of this study was to investigate the best dose used as a decontamination method as well as discover the most prevalent fungi found in this product and changes on physical properties. Samples of rice paper sheet were irradiated with 2.5, 5.0 and 10.0 kGy using a 60Co irradiator. Irradiation appeared as a safe alternative as a decontamination method without adverse effects on the physical properties in the final products.
Detection of Phakopsora pachyrhizi by polymerase chain reaction (PCR) and use of germination test and DNA comet assay afther e-beam processing in soybean
2007 - VILLAVICENCIO, A.L.C.H.; FANARO, G.B.; ARAUJO, M.M.; AQUINO, S.; SILVA, P.V.; MANCINI FILHO, J.
Soybean harvest is the main agribusiness in Brazil, which is the second largest exporter in the world and has a revenue of billions of dollars. Asian dust is caused by the fungus Phakopsora pachyrhizi and its dissemination is difficult to control, since it occurs through wind dispersion. Actually P. pachyrhizi is found in different parts of the world. Electron beam treatment could be an alternative process to minimize these losses, especially for the grains exportation industry. Besides the possibility of being disconnected when not in use, this source does not need to be reloaded, is easily available and, streamlines the process and reduces logistics costs. The present work aims to identify, by the polymerase chain reaction technique (PCR), the P. pachyrhizi fungus presence in the irradiated soybeans and the possibility to use radiation treatment as a sanitary alternative. Doses 0, 1.0, 2.0, 5.0, 6.0, 7.0, 8.0, 9.0 and 10.0 kGy (IPEN-CNEN/SP Electron Accelerator) were applied and two fast-screening methods were performed: DNA comet assay (for the detection of DNA damage) and germination test (for the measurement of roots inhibition). These tests are very easy to carry out and measure damage response depending on radiation dose.