ALLAN BERECZKI
2 resultados
Resultados de Busca
Agora exibindo 1 - 2 de 2
Artigo IPEN-doc 30048 Micro-Raman spectroscopy identification of hydroxyapatite in dental pulp stem cells2023 - SILVA, FLAVIA R.O.; PASCOAL, DIEGO R.C.; BERECZKI, ALLAN; SIPERT, CARLA R.; BRAGA, ROBERTO R.; BELLINI, MARIA H.; SILVA, LUIS F.T.; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U.Cell differentiation using calcium phosphate nanoparticles was studied. The hydroxyapatite was internalized in human dental pulp stem cells and characterized by Raman spectroscopy. Raman spectra showed the hydroxyapatite distribution in nanoparticles nodules in the cells.Resumo IPEN-doc 29318 Micro-Raman spectroscopy characterization of dental pulp stem cells differentiation induced by calcium phosphate nanoparticles2022 - SILVA, FLAVIA R.O.; PASCOAL, DIEGO R.C.; BERECZKI, ALLAN; SIPERT, CARLA R.; BRAGA, ROBERTO R.; BELLINI, MARIA H.; SILVA, LUIS F.T. da; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U.Calcium phosphates are chemical compounds used in medicine for tissue engineering. This work analyzes the process of cell differentiation by nanoparticles in dental pulp stem cells for tissues regeneration and the development of new therapeutic methods. The most widely used synthetic calcium phosphate based bioceramic is hydroxyapatite [HA, Ca10(PO4)6(OH)2]. Micro-Raman spectroscopy assays are a powerful tool for measuring and characterizing calcium phosphates nanoparticles internalized by cells because of its capability to detect the chemical bonds of nanoparticles and collagen simultaneously and evidencing their interaction within the cell-nanoparticle system. Microscope images (Fig.1a) and Raman spectra (Fig.1b) were obtained for HA-incorporated stem cells where it was possible to observe the formed nodules of calcium phosphate and the matrix in the incorporated samples. HA and collagen peaks were detected in the samples, showing that the nanoparticles induced osteogenic differentiation of the stem cells. The spectra of the nodules showed HA characteristic peaks, while matrix spectra displayed characteristic collagen peaks. Studies have been carried out for the development of new and modified calcium phosphate nanoparticles that should further improve biostimulation.