ALLAN BERECZKI
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Artigo IPEN-doc 30048 Micro-Raman spectroscopy identification of hydroxyapatite in dental pulp stem cells2023 - SILVA, FLAVIA R.O.; PASCOAL, DIEGO R.C.; BERECZKI, ALLAN; SIPERT, CARLA R.; BRAGA, ROBERTO R.; BELLINI, MARIA H.; SILVA, LUIS F.T.; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U.Cell differentiation using calcium phosphate nanoparticles was studied. The hydroxyapatite was internalized in human dental pulp stem cells and characterized by Raman spectroscopy. Raman spectra showed the hydroxyapatite distribution in nanoparticles nodules in the cells.Artigo IPEN-doc 30047 Direct nanoplastics detection below the diffraction limit using micro Raman2023 - BERECZKI, ALLAN; DIPOLD, JESSICA; FREITAS, ANDERSON Z.; WETTER, NIKLAUSRaman spectra of polystyrene nanoparticles of 50 nm diameter were directly measured using micro-Raman spectroscopy. Data analysis demonstrated that particles as small as 20 nm could be directly measured with this simple and robust technique.Artigo IPEN-doc 30037 Sub-10 nm nanoparticle detection using multi-technique-based micro-raman spectroscopy2023 - BERECZKI, ALLAN; DIPOLD, JESSICA; FREITAS, ANDERSON Z.; WETTER, NIKLAUS U.Microplastic pollution is a growing public concern as these particles are ubiquitous in various environments and can fragment into smaller nanoplastics. Another environmental concern arises from widely used engineered nanoparticles. Despite the increasing abundance of these nanosized pollutants and the possibility of interactions with organisms at the sub cellular level, with many risks still being unknown, there are only a few publications on this topic due to the lack of reliable techniques for nanoparticle characterization. We propose a multi-technique approach for the characterization of nanoparticles down to the 10 nm level using standard micro-Raman spectroscopy combined with standard atomic force microscopy. We successfully obtained single-particle spectra from 25 nm sized polystyrene and 9 nm sized TiO2 nanoparticles with corresponding mass limits of detection of 8.6 ag (attogram) and 1.6 ag, respectively, thus demonstrating the possibility of achieving an unambiguous Raman signal from a single, small nanoparticle with a resolution comparable to more complex and time-consuming technologies such as Tip-Enhanced Raman Spectroscopy and Photo-Induced Force Microscopy