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Artigo IPEN-doc 24768 Radiolabeled GX1 peptide for tumor angiogenesis imaging2018 - OLIVEIRA, ERICA A. de; FAINTUCH, BLUMA L.; SEO, DANIELE; BARBEZAN, ANGELICA B.; FUNARI, ANA; TARGINO, ROSELAINE C.; MORO, ANA M.Early and accurate detection of primary or metastatic tumors is of great value in staging, treatment management, and prognosis. Tumor angiogenesis plays an essential role in the growth, invasion, and metastatic spread of solid cancers, and so, is a promising approach for tumor imaging. The GX1 (CGNSNPKSC) peptide was identified by phage display library and has been investigated as a marker for human cancers. This study aims to evaluate the 99mTc-HYNIC-PEG4-c (GX1) as a biomarker for tumor imaging. Our results showed that GX1 specifically binds to tumor cells in vitro. SKMEL28 and MDA-MB231 cells achieved total binding peak at 60 min of incubation. For B16F10 and MKN45 cells, the total and specific binding were similar during all time points, while A549 cell line showed rapid cellular total uptake of the tracer at 30 min of incubation. Biodistribution showed low non-specific uptakes and rapid renal excretion. Melanoma tumors showed enhanced GX1 uptake in animal model at 60 min, and it was significantly blocked by cold peptide. The radiotracer showed tumor specificity, especially in melanomas that are highly vascularized tumors. In this sense, it should be considered in future studies, aiming to evaluate degree of angiogenesis, progression, and invasion of tumors.Artigo IPEN-doc 23935 Pancreas and liver uptake of new radiolabeled incretins (GLP-1 and Exendin-4) in models of diet-induced and diet-restricted obesity2017 - SEO, DANIELE; FAINTUCH, BLUMA L.; OLIVEIRA, ERICA A. de; FAINTUCH, JOELIntroduction: Radiolabeled GLP-1 and its analog Exendin-4, have been employed in diabetes and insulinoma. No protocol in conventional Diet-Induced Obesity (DIO), and Diet-Restricted Obesity (DRO), has been identified. Aiming to assess pancreatic beta cell uptake in DIO and DRO, a protocol was designed. Methods: GLP-1-βAla-HYNIC and HYNIC-βAla-Exendin-4 were labeled with technetium-99m. Four Swiss mouse models were adopted: Controls (C), Alloxan Diabetes Controls (ADC), DIO and DRO. Biodistribution and ex-vivo planar imaging were documented. Results: Radiolabeling yield was in the range of 97% and both agents were hydrophilic. Fasting Blood Glucose (FBG) was 79.2 ± 8.2 mg/dl in C, 590.4 ± 23.3 mg/dl in ADC, 234.3 ± 66.7 mg/dl in DIO, and 96.6 ± 9.3 in DRO (p = 0.010). Biodistribution confirmed predominantly urinary excretion. DIO mice exhibited depressed uptake in liver and pancreas, for both radiomarkers, in the range of ADC. DRO only partially restored such values. 99mTc-HYNIC-βAla-Exendin-4 demonstrated better results than GLP-1-βAla-HYNIC-99mTc. Conclusions: 1) Diet-induced obesity remarkably depressed beta cell uptake; 2) Restriction of obesity failed to normalize uptake, despite robust improvement of FBG; 3) HYNIC-βAla-Exendin-4 was the most useful marker; 4) Further studies are recommended in obesity and dieting, including bariatric surgery.Artigo IPEN-doc 13919 Determinação de urânio em amostras de mexilhões Perna perna transplantados ao longo do litoral de São Paulo, por análise por ativação com neutrons epitérmiços (AANE)2008 - SEO, D.; CATHARINO, M.G.M.; MOREIRA, E.G.; SAIKI, M.; VASCONCELLOS, M.B.A.; PEREIRA, C.D.S.; SOUSA, E.C.P.M.Moluscos bivalves vêm sendo utilizados em estudos de biomonitoração de regiões costeiras, pois são capazes de acumular poluentes. Este trabalho apresenta resultados das determinações de urânio em amostras de mexilhões, da espécie Perna perna (Linnaeus, 1758), transplantados ao longo do litoral norte do Estado de São Paulo. Mexilhões provenientes de um cultivo situado na Praia da Cocanha, Caraguatatuba (controle), foram transplantados em todas as estações do ano para quatro pontos de transplantes: Praia do Engenho d’Água, em Ilhabela; Terminal Almirante Barroso (TEBAR), em São Sebastião; e Ilha das Palmas e Itaipu, na Baía de Santos. Para as determinações de urânio foi utilizada a técnica de análise por ativação com nêutrons epitérmicos (AANE), e para controle da qualidade dos resultados foram analisados os materiais de referência certificados NIST SRM1566b Oyster Tissue e NIST SRM 1575 Pine Needles. Os resultados obtidos foram concordantes com os valores dos certificados, com percentagens de erro relativos inferiores a 2,2%, e apresentaram boa precisão, com desvios-padrão relativos inferiores a 3,3%. A concentração de urânio variou de 40,8 a 101,1 µg kg–1 (base seca) nas amostras de mexilhão analisadas. A comparação entre as concentrações de U obtidas nos mexilhões dos pontos de transplante indicou que a região de Santos possui a maior e a menor concentração de U, dependendo do período de exposição das amostras de mexilhão.Artigo IPEN-doc 17880 Biomonitoring of coastal regions of São Paulo state, Brazil, using mussels Perna perna2012 - CATHARINO, M.G.M.; VASCONCELLOS, M.B.A.; KIRSCHBAUM, A.A.; GASPARRO, M.R.; MINEI, C.C.; SOUSA, E.C.P.M. de; SEO, D.; MOREIRA, E.G.Artigo IPEN-doc 18063 Vanadium biomonitoring by using Perna perna (Linnaeus, 1758) mussels transplanted in the coast of the State os São Paulo, Brazil2012 - SEO, D.; VASCONCELLOS, M.B.A.; SAIKI, M.; CATHARINO, M.G.M.; MOREIRA, E.G.; SOUSA, E.C.P.M. de; PEREIRA, C.D.S.Artigo IPEN-doc 18757 Vanadium determination in Perna perna mussels (Linnaeus, 1758: Mollusca, Bivalvia) by instrumental neutron activation analysis using the passive biomonitoring in the Santos coast, Brazil2013 - SEO, D.; VASCONCELLOS, M.B.A.; CATHARINO, M.G.M.; MOREIRA, E.G.; SOUSA, E.C.P.M. de; SAIKI, M.Vanadium determination present in seawater is of great importance to evaluate marine contamination from industrial sources as well as to identify health hazards since mussels and other marine organisms are used as food. Besides, this evaluation in the Santos coast, SP, Brazil, is important, since this area is impacted by industrial and urban activities and discharges from ships and boats. In a previous study, V results obtained for transplanted mussel (active biomonitoring) were presented. This study aimed the V determination by passive biomonitoring by analyzing Perna perna mussels collected in natural environment, from three sites in Sa˜o Paulo State coast: Cocanha Beach (reference site), Ponta de Itaipu and Palmas Island. Ninety individuals of mussels were collected in each site between September/08 and July/09 during the four seasons of the year. After shell removal and sample preparation, V was determined by instrumental neutron activation analysis. For analytical quality control, the NIST Standard Reference Material SRM 1566b Oyster Tissue was analyzed and the results presented good accuracy. The V concentrations in mussels on dry mass basis varied from 0.77 ± 0.02 to 3.56 ± 0.88 mg kg-1 . Statistical tests were applied to the results showing differences on V concentrations among the sampling sites and season of sample collection.Artigo IPEN-doc 18840 Trace element determination in a mussel reference material using short irradiation instrumental neutron activation analysis2013 - MOREIRA, E.G.; SEO, D.; VASCONCELLOS, M.B.A.; SAIKI, M.