MARYCEL ELENA BARBOZA COTRIM

MARYCEL ELENA BARBOZA COTRIM

(Fonte: Lattes)

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Bachelor's at Química from Faculdades Osvaldo Cruz (1985), master's at Tecnologia Nuclear from Instituto de Pesquisas Energeticas E Nucleares (1991) and doctorate at Tecnologia Nuclear from Instituto de Pesquisas Energeticas E Nucleares (2006). Has experience in Chemistry, focusing on Separation, acting on the following subjects: avaliação ambiental, metais, química ambiental, qualidade da água and química analítica. (Text obtained from the Currículo Lattes on November 17th 2021)

Mestrado em Tecnologia Nuclear pelo Instituto de Pesquisas Energéticas E Nucleares (1991) e Doutorado em Tecnologia Nuclear pelo Instituto de Pesquisas Energéticas E Nucleares (2006). Atualmente é pesquisador da Comissão Nacional de Energia Nuclear. Experiência em química analítica, atuando principalmente nos seguintes temas: avaliação ambiental, metais, química ambiental, qualidade da água e química analítica, caracterização de compostos de urânio e química analítica instrumental. (Texto extraído do Currículo Lattes em 17 nov. 2021)

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Carbon allocation of Spirodela polyrhiza under boron toxicity
2023 - PAGLIUSO, DEBORA; PEREIRA, JOAO P. de J.; ULRICH, JOAO C.; COTRIM, MARYCEL E.B.; BUCKERIDGE, MARCOS S.; GRANDIS, ADRIANA
Pectic polysaccharides containing apiose, xylose, and uronic acids are excellent candidates for boron fixation. Duckweeds are the fastest-growing angiosperms that can absorb diverse metals and contaminants from water and have high pectin content in their cell walls. Therefore, these plants can be considered excellent boron (B) accumulators. This work aimed to investigate the relationship between B assimilation capacity with apiose content in the cell wall of Spirodela polyrhiza subjected to different boric acid concentrations. Plants were grown for 7 and 10 days in ½ Schenck-Hildebrandt media supplemented with 0 to 56 mg B.L-1, the non-structural and structural carbohydrates, and related genes were evaluated. The results showed that B altered the morphology and carbohydrate composition of this species during plant development. The optimum B concentration (1.8 mg B.L-1) led to the highest relative growth and biomass accumulation, reduced starch, and high pectin and apiose contents, together with increased expression of UDP-apiose/UDP-xylose synthase (AXS) and 1,4-α-galacturonosyltransferase (GAUT). The toxic state (28 and 56 mg B.L-1) increased the hexose contents in the cell wall with a concomitant reduction of pectins, apiose, and growth. The pectin content of S. polyrhiza was strongly associated with its growth capacity and regulation of B content within the cells, which have AXS as an important regulator. These findings suggest that duckweeds are suitable for B remediation, and their biomass can be used for bioenergy production.
Disponibilidade de elementos traço para absorção cutânea em tratamentos com a lama negra de Peruíbe
2022 - TORRECILHA, JEFFERSON K.; GOUVEA, PAULO F. de M.; COTRIM, MARYCEL E.B.; SILVA, PAULO S.C. da
Introdução: No Brasil, o uso de recursos naturais passou a fazer parte do Sistema Único de Saúde (SUS) com a implantação da “Política Nacional de Práticas Integrativas e Complementares” e a Lama Negra, localizada na cidade de Peruíbe, Estado de São Paulo, tem sido amplamente utilizado para tratamentos terapêuticos, por exemplo psoríase, dermatite periférica, neuropatia, acne e seborreia, mialgia, artrite e processos reumáticos não articulares. Objetivo: O objetivo deste estudo foi verificar a mobilidade dos elementos da Lama Negra de Peruíbe, e avaliar quais (benéficos ou tóxicos) estão disponíveis para transferência ao paciente durante o tratamento terapêutico tópico. Para tanto, foram empregados dois métodos de extração: procedimento de extração sequencial e extração única com emprego de suor artificial. Métodos: Dois métodos de extração foram empregados para medir os elementos extraídos: espectroscopia de emissão atômica com plasma indutivamente acoplado e espectrometria de absorção atômica com forno de grafite. Resultados e discussão: Os resultados mostraram que os elementos Ca, Cd, Mg, Mn e Na são altamente extraídos na fração trocável e resultados semelhantes foram observados na extração com suor artificial, entretanto, isso pode não ser um problema durante o tratamento terapêutico. Conclusão: Todos os outros elementos investigados foram extraídos em baixas concentrações, indicando que os efeitos adversos à saúde devem ser desprezíveis, embora até o momento haja pouca ou nenhuma evidência de absorção pela pele.
Pb removal from water by three types of biofilters
2022 - BORDON, ISABELLA C.; CAMPOS, BRUNO G.; MIYAI, CAIO A.; SILVA, JOSE R.M.C. da; COTRIM, MARYCEL E.B.; FAVARO, DEBORAH I.T.; ABESSA, DENIS M.S.
Introduction: As aquatic organisms are directly affected by water quality, the use and evaluation of biofilters aiming at the previous removal of Pb guarantee the adequate water physicochemical parameters to the maintenance of the organisms of interest. Objective: This note aims to report the results of a water pre-treatment testing three types of biofilters (river gravel and two types of seashell-based media: self-collected and commercial) to evaluate their ability to absorb and consequently remove the Pb from water. M&M: Temperature and salinity were measured using Hg thermometers and a refractometer, respectively. Commercial colorimetric test kits were used to monitor pH and the toxic ammonia concentration. The Pb determination was performed by using GF AAS analytical technique. Results: According to results, biofilters can significantly remove concentrations of Pb from the water column. The evaluated biofilters kept the water physical-chemical parameters stable. Results support the use of these biofilters to treat water before any experiment. Conclusion: This report may subside future studies on the use of these biofilters in other activities, such as aquaculture and waste treatment, to maintain the physical-chemical water parameters well-controlled. Statement of Novelty: The present study has tested in laboratory conditions, three types of biofilters (river gravel and two types of seashell-based media: self-collected and commercial) to evaluate their ability to absorb and consequently remove the Pb. This report may subside studies on the use of these biofilters in activities, such as aquaculture and waste treatment, to maintain the physical-chemical water parameters well-controlled, applying low-cost biomaterials.
Talaromyces amestolkiae uses organic phosphate sources for the treatment of uranium‑contaminated water
2022 - COELHO, EDNEI; REIS, TATIANA A.; COTRIM, MARYCEL; MULLAN, THOMAS K.; RENSHAW, JOANNA; RIZZUTTO, MARCIA; CORREA, BENEDITO
Fungi have received particular attention in regards to alternatives for bioremediation of heavy metal contaminated locales. Enzymes produced by filamentous fungi, such as phosphatases, can precipitate heavy metal ions in contaminated environments, forming metal phosphates (insoluble). Thus, this research aimed to analyze fungi for uranium biomineralization capacity. For this, Gongronella butleri, Penicillium piscarium, Rhodotorula sinensis and Talaromyces amestolkiae were evaluated. Phytate and glycerol 2-phosphate were used as the phosphate sources in the culture media at pH 3.5 and 5.5, with and without uranium ions. After 4 weeks of fungal growth, evaluated fungi were able to produce high concentrations of phosphates in the media. T. amestolkiae was the best phosphate producer, using phytate as an organic source. During fungal growth, there was no change in pH level of the culture medium. After 3 weeks of T. amestolkiae growth in medium supplemented with phytate, there was a reduction between 20 and 30% of uranium concentrations, with high precipitation of uranium and phosphate on the fungal biomass. The fungi analyzed in this research can use the phytic acid present in the medium and produce high concentrations of phosphate; which, in the environment, can assist in the heavy metal biomineralization processes, even in acidic environments. Such metabolic capabilities of fungi can be useful in decontaminating uranium-contaminated environments.
Microscale Toxicity Identification Evaluation (TIE) for interstitial water of estuarine sediments affected by multiple sources of pollution
2022 - ALVES, ALINE V.; FERRAZ, MARIANA A.; MORENO, BEATRIZ B.; NOBRE, CAIO R.; ANTUNES, RENATA M.; PUSCEDDU, FABIO H.; BORDON, ISABELLA C. da C.L.; FAVARO, DEBORAH I.T.; COTRIM, MARYCEL E.B.; CHOUERI, RODRIGO B.
Estuaries in the world are affected by different contamination sources related to urbanisation and port/industrial activities. Identifying the substances responsible for the environmental toxicity in estuaries is challenging due to the multitude of stressors, both natural and anthropogenic. The Toxicity Identification and Evaluation (TIE) is a suitable way of determining causes of toxicity of sediments, but it poses difficulties since its application is labour intensive and time consuming. The aimof this study is to evaluate the diagnosis provided by a TIE based on microscale embryotoxicity tests with interstitial water (IW) to identify toxicants in estuarine sediments affected by multiple stressors. TIE showed toxicity due to different combinations of metals, apolar organic compounds, ammonia and sulphides, depending on the contamination source closest to the sampling station. The microscale TIE was able to discern different toxicants on sites subject to different contamination sources. There is good agreement between the results indicated in the TIE and the chemical analyses in whole sediment, although there are some disagreements, either due to the sensitivity of the test used, or due to the particularities of the use of interstitial water to assess the sediment toxicity. The improvement of TIE methods focused on identifying toxicants in multiple-stressed estuarine areas are crucial to discern contamination sources and subsidise management strategies.
Bioremediation of water contaminated with uranium using Penicillium piscarium
2020 - COELHO, EDNEI; REIS, TATIANA A.; COTRIM, MARYCEL; RIZZUTTO, MARCIA; CORREA, BENEDITO
Penicillium piscarium can be indicated as promising in the treatment of sites contaminated with uranium. Thus, this research aimed to analyze the P. piscarium dead biomass in uranium biosorption. This fungus was previously isolated from a highly contaminated uranium mine located in Brazil. Biosorption tests were carried out at pH 3.5 and 5.5 in solutions contaminated with concentrations of 1 to 100 mg/L of uranium nitrate. Our results showed that the dead biomass of P. piscarium was able to remove between 93.2 and 97.5% uranium from solutions at pH 3.5, at the end of the experiment, the pH of the solution increased to values above 5.6. Regarding the experiments carried out in solutions with pH 5.5, the dead biomass of the fungus was also able to remove between 38 and 92% uranium from the solution, at the end of the experiment, the pH of the solution increased to levels above 6.5. The analysis of electron microscopy, Energy-dispersive spectroscopy, and X-ray fluorescence demonstrated the high concentration of uranium precipitated on the surface of the fungal biomass. These results were impressive and demonstrate that the dead biomass of P. piscarium can be an important alternative to conventional processes for treating water contaminated with heavy metals, and we hope that these ecofriendly, inexpensive, and effective technologies be encouraged for the safe discharge of water from industrial activities.
Resistant fungi isolated from contaminated uranium mine in Brazil shows a high capacity to uptake uranium from water
2020 - COELHO, EDNEI; REIS, TATIANA A.; COTRIM, MARYCEL; MULLAN, THOMAS K.; CORREA, BENEDITO
The Osamu Utsumi uranium mine occupies a 20 km2 area in the city of Caldas, which is located in the state of Minas Gerais, Brazil. Since mining activities ended at Osamu Utsumi 24 years ago, the surrounding area has become contaminated by acid effluents containing high concentrations of uranium. Thus, the aim of this study was to assess the uranium bioremediation capacity of 57 fungi isolated from the mine area. In tolerance tests, 38% (22) of the fungal isolates were considered tolerant to uranium, including 10 Penicillium species. At a uranium concentration of 2000 mg L 1 48 fungi did not exhibit mycelial growth index inhibition. Minimal inhibitory concentration (MIC) analysis showed growth of 25 fungi above a uranium concentration of 8000 mg L 1. At high uranium concentrations, some fungi (i.e., Talaromyces amestolkiae and Penicillium citrinum) showed morphological changes and pigment (melanin) production. Among the fungal isolates, those considered to be more tolerant to uranium were isolated from soil and sediment samples containing higher concentrations of heavy metal. When comparing the results of resistance/tolerance tests with those for uranium biosorption capacity, we concluded that the fungi isolated from the Osamu Utsumi mine with the best potential for uranium bioremediation were Gongronella butleri, Penicillium piscarium, Penicillium citrinum, Penicillium ludwigii, and Talaromyces amestolkiae. Biosorption tests with live fungal biomass showed that 11 species had a high potential for uranium uptake from contaminated water.
Improvements in metal exposure assays
2019 - BORDON, ISABELLA C.; CAMPOS, BRUNO G. de; GUSSO‑CHOUERI, PALOMA K.; MIYAI, CAIO A.; ARAUJO, GIULIANA S. de; EMERENCIANO, ANDREWS K.; SILVA, JOSE R.M.C. da; COTRIM, MARYCEL E.B.; FAVARO, DEBORAH I.T.; ABESSA, DENIS M. de S.
This technical note aims to report, in detail, the development, suitability and analytical validation of an artificial food to assess metal bioaccumulation in the blue crab Callinectes danae after metal exposure assays. For this purpose, lead (Pb) was chosen as the metal of interest. First, a non-contaminated artificial food was prepared by mixing commercial shrimps and agarose. The food was then offered on alternate days to two groups of crabs for 7 and 14 days, respectively. After confirming that this food was well-accepted by crabs, analytical procedures were performed to confirm that Pb added to the mixture would remain in the artificial food. For this purpose, two foods containing Pb at concentrations of 0.5 and 2.0 μg g− 1 were prepared by adding aliquots of an ultrapure Pb standard solution to the mixture. Samples of each food were subjected to an acid extraction and Pb concentrations were determined by a GF-AAS. Pb recoveries in both contaminated foods were > 90%. Subsequently, another experiment was performed, and two groups of crabs were fed with contaminated artificial foods (0.5 and 2.0 μg g− 1 of Pb) during 7 and 14 days, respectively. The observed acceptance of these foods by crabs at both Pb concentrations was of 100%.
Top down arsenic uncertainty measurement in water and sediments from Guarapiranga dam (Brazil)
2018 - FAUSTINO, M.G.; LANGE, C.N.; MONTEIRO, L.R.; FURUSAWA, H.A.; MARQUES, J.R.; STELLATO, T.B.; SOARES, S.M.V.; SILVA, T.B.S.C. da; SILVA, D.B. da; COTRIM, M.E.B.; PIRES, M.A.F.
Total arsenic measurements assessment regarding legal threshold demands more than average and standard deviation approach. In this way, analytical measurement uncertainty evaluation was conducted in order to comply with legal requirements and to allow the balance of arsenic in both water and sediment compartments. A top-down approach for measurement uncertainties was applied to evaluate arsenic concentrations in water and sediments from Guarapiranga dam (São Paulo, Brazil). Laboratory quality control and arsenic interlaboratory tests data were used in this approach to estimate the uncertainties associated with the methodology.
Mechanical characterization and ion release of bioactive dental composites containing calcium phosphate particles
2018 - NATALE, LIVIA C.; RODRIGUES, MARCELA C.; ALANIA, YVETTE; CHIARI, MARINA D.S.; BOARO, LETICIA C.C.; COTRIM, MARYCEL; VEGA, OSCAR; BRAGA, ROBERTO R.
Objective: to verify the effect of the addition of dicalcium phosphate dihydrate (DCPD) particles functionalized with di- or triethylene glycol dimethacrylate (DEGDMA or TEGDMA) on the degree of conversion (DC), post-gel shrinkage (PS), mechanical properties, and ion release of experimental composites. Methods: Four composites were prepared containing a BisGMA/TEGDMA matrix and 60 vol% of fillers. The positive control contained only barium glass fillers, while in the other composites 15 vol% of the barium was replaced by DCPD. Besides the functionalized particles, non-functionalized DCPD was also tested. DC after 24 h (n=3) was determined by FTIR spectroscopy. The strain gage method was used to obtain PS 5 min after photoactivation (n=5). Flexural strength and modulus (n=10) were calculated based on the biaxial flexural test results, after specimen storage for 24 h or 60 days in water. The same storage times were used for fracture toughness testing (FT, n=10). Calcium and phosphate release up to 60 days was quantified by ICP-OES (n=3). Data were analyzed by ANOVA/Tukey test (alpha: 5%). Results: Composites containing functionalized DCPD presented higher DC than the control (p < 0.001). The material containing DEGDMA-functionalized particles showed higher PS than the other composites (p < 0.001). After 60 days, only the composite with DEGDMA-functionalized DCPD presented fracture strength similar to the control, while for flexural modulus only the composite with TEGDMA-functionalized particles was lower than the control (p < 0.001). FT of all composites containing DCPD was higher than the control after 60 days (p < 0.005). Calcium release was higher for the composite with non-functionalized DCPD at 15 days and no significant reductions were observed for composites with functionalized DCPD during the observation period (p < 0.001). For all the tested composites, phosphate release was higher at 15 days than in the subsequent periods, and no difference among them was recorded at 45 and 60 days (p < 0.001). Conclusions: DCPD functionalization affected all the studied variables. The composite with DEGDMA-functionalized particles was the only material with strength similar to the control after 60 days in water; however, it also presented the highest shrinkage. The presence of DCPD improved FT, regardless of functionalization. DCPD functionalization reduced ion release only during the first 15 days.