KELME CARDOSO DAMASCENO
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Artigo IPEN-doc 25728 In vitro and in vivo toxicity of coal fly Ash Lechatee2018 - DAMASCENO, K.C.; CAVALCANTE, A.K.; MAZIERO, J.S.; MARTINI, G.A.; ORMENIO, M.B.; MAMEDE, F.C.; MIRANDA, C.S.; CAMPELLO, F.A.; IZIDORO, J.C.; ROGERO, S.O.; FUNGARO, D.A.; LOPES-FERREIRA, M.; ROGERO, J.R.Coal Fly ash is a major solid waste from coal-fired power stations. In Brazil, more than 4 million tons per year of fly ash are generated and only 30% is applied as raw material for cement and concrete production. The remaining is disposed in on-site ponds, nearby abandoned or active mine sites and landfills. The inadequate disposal of fly ash may pose a significant risk to the environment due to the possible leaching of hazardous pollutants into the surrounding soil and groundwater. A combination of leaching tests, cytotoxicity and ecotoxicological assays were used in this studyin order toevaluate the possible adverse effects of coal fl y ash in non-target organisms. The sample was collected from coal-fi red power plant located in Southern Brazil and the coal fly ash was submitted to a leaching procedure using USEPA SW 864 Method 1311. The leachate was prepared in six dilutions: 1.56%, 3.12%, 6.25%, 12.5%, 25% and 50%. Acute toxicity tests were performed on NCTC clone 929 (CCIAL-020) culture cells by neutral red uptake cytotoxicity method; acute ecotoxicity usingDaphnia similisand Danio rerio embryos according to ABNT NBR 12713 and OECD 236, respectively were employed. The cytotoxicity index (CI50) obtained was 33%; the EC50of D. similis after 48 h of exposure to the leachate was 7.25% and the LC50of D. rerio after 96 h of exposure was 4.39%. The results of these bioassays indicated toxicity of the coal fly ash leachate toward exposed organisms.Artigo IPEN-doc 24145 Resveratrol radiomodifier effect on Danio rerio embriolarval assay2017 - DAMASCENO, KELME C.; MAMEDE, FERNANDA C.S.; CAVALCANTE, ADRIANA K.; ROGERO, SIZUE O.; FERREIRA, MONICA L.; ROGERO, JOSE R.The ionizing radiation can cause fatal damages to cells by the direct interaction with DNA and RNA or a series of toxic reactions occasioning chemical and biological changes. There are compounds with radioprotective potential, like resveratrol. For use the organism. Resveratrol is a substance found in peanuts, grapes and wine and its production occurs in plants as a response to physical, chemical and biological stress. Some studies benefits. Danio rerio (zebrafish) is a vertebrate animal and has become the model of several studies related to human diseases, due to its genomes similarity of 70 %, rapid embryonic development and the transparency o the eggs, which make it possible to observe the effects during the test period. The aim of the present study to verify the resveratrol radiomodifier effect on zebrafish during the embryolarval development by modified Fish Embryo Acute Toxicity (FET) (LC50) was 66.9 mg.L-1. Before, to understand the effects of radiation, was carried out the gamma radiation lethal dose (LD50) assay and the LD50 was 25 Gy. With these results the projec study of the radiomodifier effect of resveratrol in the presence of gamma radiationResumo IPEN-doc 23320 Ecotoxicity evaluation of Coal Fly ash to Daphnia similis and Danio rerio2017 - CAVALCANTE, A.K.; DAMASCENO, K.C.; MAZIERO, J.S.; MARTINI, G.A.; ORMENIO, M.B.; MAMEDE, F.C.S.; MIRANDA, C.S.; IZIDORO, J.C.; CAMPELLO, F.A.; FUNGARO, D.A.; ROGERO, S.O.; ROGERO, J.R.Coal Fly ash is a major solid waste from coal-fired power stations. In Brazil, more than 4 million tons per year of fly ash are generated with a tendency to increase every year. Only 30% of fly ash is applied as raw material for cement and concrete production. The remaining is disposed in onsite ponds, nearby abandoned or active mine sites, or landfills. The inadequate disposal of fly ash may pose a significant risk to the environment due to the possible leaching of hazardous pollutants into the surrounding soil and groundwater. A combination of leaching tests and ecotoxicological analyses were used in this work for the evaluation of the adverse effects of coal fly in non-target organisms. Ashes were collected from coal-fired power plant located in South of Brazil. Acute toxicity tests were performed with Danio rerio embryos and Daphnia similis, according to OECD 236 and ABNT NBR 12713, respectively. Coal fly ash sample was subjected to a leaching procedure using USEPA SW 864 Method 1311. The leachate was prepared in seven dilutions: 1.56%, 3.12%, 6.25%, 12.5%, 25%, 50%, and 100%. The assays were performed in triplicates and the results showed lethality of Danio rerio after 96 hours of exposure to the leachate, and the calculated LC50 was 4.39%. The ecotoxicity tests with Daphnia similis, observed immobility after 48 hours of exposure to the leachate, and EC50 calculated was 7.25%. The results of these tests indicate toxicity of the coal fly ash leachate toward exposed organisms.