KELME CARDOSO DAMASCENO
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Artigo IPEN-doc 27385 Species-specific in vitro and in vivo evaluation of toxicity of silver nanoparticles stabilized with gum arabic protein2020 - MAZIERO, JOANA S.; THIPE, VELAPHI C.; ROGERO, SIZUE O.; CAVALCANTE, ADRIANA K.; DAMASCENO, KELME C.; ORMENIO, MATHEUS B.; MARTINI, GISELA A.; BATISTA, JORGE G.S.; VIVEIROS, WILLIAM; KATTI, KAVITA K.; KARIKACHERY, ALICE R.; MOHANDOSS, DARSHAKUMAR D.; DHURVAS, RASHMI D.; NAPPINNAI, MOHANAVELU; ROGERO, JOSE R.; LUGAO, ADEMAR B.; KATTI, KATTESH V.Introduction: We report, herein, in vitro, and in vivo toxicity evaluation of silver nanoparticles stabilized with gum arabic protein (AgNP-GP) in Daphnia similis, Danio rerio embryos and in Sprague Dawley rats. Purpose: The objective of this investigation was to evaluate in vitro and in vivo toxicity of silver nanoparticles stabilized with gum arabic protein (AgNP-GP), in multispecies due to the recognition that toxicity evaluations beyond a single species reflect the environmental realism. In the present study, AgNP-GP was synthesized through the reduction of silver salt using the tri-alanine-phosphine peptide (commonly referred to as “Katti Peptide”) and stabilized using gum arabic protein. Methods: In vitro cytotoxicity tests were performed according to ISO 10993– 5 protocols to assess cytotoxicity index (IC50) values. Acute ecotoxicity (EC50) studies were performed using Daphnia similis, according to the ABNT NBR 15088 protocols. In vivo toxicity also included evaluation of acute embryotoxicity using Danio rerio (zebrafish) embryos following the OECD No. 236 guidelines. We also used Sprague Dawley rats to assess the toxicity of AgNP-GP in doses from 2.5 to 10.0 mg kg− 1 body weight. Results: AgNP-GP nanoparticles were characterized through UV (405 nm), core size (20± 5 nm through TEM), hydrodynamic size (70– 80 nm), Zeta (ζ) potential (- 26 mV) using DLS and Powder X ray diffraction (PXRD) and EDS. PXRD showed pattern consistent with the Ag (1 1 1) peak. EC50 in Daphnia similis was 4.40 (3.59– 5.40) μg L− 1. In the zebrafish species, LC50 was 177 μg L− 1. Oral administration of AgNP-GP in Sprague Dawley rats for a period of 28 days revealed no adverse effects in doses of up to 10.0 mg kg− 1 b.w. in both male and female animals. Conclusion: The non-toxicity of AgNP-GP in rats offers a myriad of applications of AgNP-GP in health and hygiene for use as antibiotics, antimicrobial and antifungal agents.Resumo IPEN-doc 25278 Avaliação da toxicidade in vitro e in vivo das Nanopartículas de Prata2018 - MAZIERO, J.S.; ROGERO, S.O.; DAMASCENO, K.C.; ORMENIO, M.B.; CAVALCANTE, A.K.; LUGAO, A.B.; ROGERO, J.R.Devido às suas propriedades como tamanho, formato variado, elevada área superficial e alto poder bactericida, as nanopartículas de prata (NPAg) vem sendo amplamente utilizadas em diversos setores da indústria: curativos, devido a sua capacidade bactericida; no interior de refrigeradores de alimentos, para retardar a deterioração; em palmilhas antimicrobianas, para evitar odores; em purificadores de ar; em instrumentos cirúrgicos e etc. A sua ampla utilização tem provocado grande preocupação na área acadêmica, principalmente ecotoxicológica, quanto aos impactos e riscos potenciais que estas podem causar ao meio ambiente e à saúde humana. Baseado nessas considerações, este trabalho teve como objetivo verificar o nível de toxicidade destas NPAg utilizando ensaios in vitro e in vivo. O teste in vitro de citotoxicidade, foi realizado seguindo a norma ISO 10993 - 5 pelo método de incorporação do corante vermelho neutro, em células da linhagem NCTC-L929 e os ensaios in vivo de ecotoxicidade aguda, de acordo com a norma brasileira ABNT NBR 12713, utilizando como organismo teste a Daphnia similis. Os resultados obtidos foram o IC50 (índice de citotoxicidade, que é a concentração das NPAg que causa mortalidade a 50% das células expostas), de 2,57 mg. L-1, e a CE50 (concentração efetiva das NPAg que causa imobilidade em 50% dos organismos expostos) de 4,40 μg. L-1. Estes resultados mostram que os organismos aquáticos são muito mais sensíveis às NPAg do que as células em cultura, elevando a importância de se realizar mais estudos relacionados às adversidades que essas nanopartículas podem causar. Além disso, mostra-se necessário verificar o descarte das mesmas no meio ambiente, visto que no Brasil ainda não há legislações que quantifiquem os limites permissíveis para esse descarte.Artigo IPEN-doc 24145 Resveratrol radiomodifier effect on Danio rerio embriolarval assay2017 - DAMASCENO, KELME C.; MAMEDE, FERNANDA C.S.; CAVALCANTE, ADRIANA K.; ROGERO, SIZUE O.; FERREIRA, MONICA L.; ROGERO, JOSE R.The ionizing radiation can cause fatal damages to cells by the direct interaction with DNA and RNA or a series of toxic reactions occasioning chemical and biological changes. There are compounds with radioprotective potential, like resveratrol. For use the organism. Resveratrol is a substance found in peanuts, grapes and wine and its production occurs in plants as a response to physical, chemical and biological stress. Some studies benefits. Danio rerio (zebrafish) is a vertebrate animal and has become the model of several studies related to human diseases, due to its genomes similarity of 70 %, rapid embryonic development and the transparency o the eggs, which make it possible to observe the effects during the test period. The aim of the present study to verify the resveratrol radiomodifier effect on zebrafish during the embryolarval development by modified Fish Embryo Acute Toxicity (FET) (LC50) was 66.9 mg.L-1. Before, to understand the effects of radiation, was carried out the gamma radiation lethal dose (LD50) assay and the LD50 was 25 Gy. With these results the projec study of the radiomodifier effect of resveratrol in the presence of gamma radiation