ALVARO ROSSAN DE BRANDAO PRIETO DA SILVA
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Resumo IPEN-doc 14425 Hydrid protein endostatin-bax presents higher degree of apoptosis than endostatin after its internalization by endothelial cell line. (C-PAE)2007 - CHURA-CHAMBI, R.M.; AMARANTE MENDES, G.P.; JACYSYN, J.F.; PRIETO da SILVA, A.; MALAVASI, N.V.; RODRIGUES, D.B.; MORGANTI, L.Resumo IPEN-doc 12461 Expression of gyroxin-like in CHO-DHFR cells2007 - YONAMINE, C.M.; SILVA, A.R.B.P. da; MORGANTI, L.; MAGALHAES, G.S.; CHURA-CHAMBI, R.M.; CAMILLO, M.A.P.; BAPTISTA, G.R.; YAMANE, T.Artigo IPEN-doc 20121 Improving the therapeutic potential of endostatin by fusing it the BAX BH3 death domain2014 - CHURA-CHAMBI, R.M.; BELLINI, M.H.; JACYSYN, J.F.; ANDRADE, L.N.; MEDINA, L.P.; PRIETO da SILVA, A.R.B.; AMARANTE-MENDES, G.P.; MONGANTI, L.Endostatin (ES) inhibits angiogenesis, reducing tumor growth in animal models. However, it has low therapeutic effect in human clinical trials. BAX is a member of the BCL-2 family of proteins; its proapoptotic (BH3) domain interacts with other members of the family in the cytoplasm, to induce apoptosis. Here, we fused the BAX BH3 domain with murine ES, to enhance ES potency. Endothelial cells specifically internalize the fusion protein ES-BAX. The presence of the BAX domain enhances endothelial cell death by apoptosis by 1.8-fold and diminishes microvessel outgrowth in the rat aortic ring assay by 6.5-fold. Daily injections of 15 lg of ES-BAX/g in tumor-bearing mice reduce tumor weight by 86.9% as compared with ES-treated animals. Co-immunoprecipitation assays confirmed that ES-BAX interacts with members of the BCL-2 family. Also, ES interacts with BCL-2, BCL-XL, and BAK in endothelial cell lysates, suggesting a potential new mechanism for the apoptosis induction by ES. The superiority of the ES-BAX antiangiogenic effect indicates that this fusion protein could be a promising therapeutic alternative to treat cancer.Resumo IPEN-doc 13667 Cloning and sequence analysis of gyroxin-like from Crotalus durissus terrificus venom gland2007 - YONAMINE, C.M.; PRIETO da SILVA, A.R.B.; YASSAKA, R.T.; CAMILLO, M.A.P.; RADIS BAPTISTA, G.; YAMANE, T.Artigo IPEN-doc 14737 Cloning of serine protease cDNAs from Crotalus durissus terrificus venom gland and expression of a functional gyroxin homologue in COS-7 cells2009 - YONAMINE, C.M.; PRIETO da SILVA, A.R.B.; MAGALHAES, G.S.; RADIS BAPTISTA, G.; MORGANTI, L.; AMBIEL, F.C.; CHURA-CHAMBI, R.M.; YAMANE, T.; CAMILLO, M.A.P.