EUTIMIO GUSTAVO FERNANDEZ NUNEZ
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Artigo IPEN-doc 23936 Radiotracers for different angiogenesis receptors in a melanoma model2012 - OLIVEIRA, ERICA A.; FAINTUCH, BLUMA L.; NUNEZ, EUTIMIO G.F.; MORO, ANA M.; NANDA, PRASANT K.; SMITH, CHARLES J.Early and reliable diagnosis of melanoma, a skin tumor with a poor prognosis, is extremely important. Phage display peptide libraries are a convenient screening resource for identifying bioactive peptides that interact with cancer targets. The aim of this study was to evaluate two technetium-99m tracers for angiogenesis detection in a melanoma model, using cyclic pegylated pentapeptide with RGD and NGR motifs conjugated with the bifunctional chelator mercaptoacetyltriglycine (MAG3). The conjugated peptides (10 ll of a lg/ll solution) were labeled with technetium-99m using a sodium tartrate buffer. Radiochemical evaluation was carried out by instant thin-layer chromatography and confirmed by high-performance liquid chromatography. The partition coefficient was determined and internalization assays were performed in two melanoma cell lines (B16F10 and SKMEL28). Biodistribution evaluation of the tracers was carried out in healthy animals at different time points and also in tumor-bearing mice, 120 min post injection. Blocking studies were also conducted by coinjection of cold peptides. The conjugates displayed a rather similar pharmacokinetic profile. They were radiolabeled with high radiochemical purity (> 97%) and both were hydrophilic with preferential renal excretion. Yet, tumor uptake was higher for human than for murine melanoma cells, especially for [99mTc]-MAG3-PEG8-c(RGDyk) (7.85± 2.34%injected dose/g 120 min post injection). The performance of [99mTc]-MAG3-PEG8-c(RGDyk) was better than the NGR tracer with regard to human melanoma uptake. In this sense, it should be considered for future radiotracer studies of tumor diagnosis. Melanoma Res 22:45–53 c 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.Artigo IPEN-doc 18913 Radiotracers for different angiogenesis receptors in a melanoma model2012 - OLIVEIRA, ERICA A.; FAINTUCH, BLUMA L.; NUNEZ, EUTIMIO G.F.; MORO, ANA M.; NANDA, PRASANT K.; SMITH, CHARLES J.Artigo IPEN-doc 14527 Influence of colloid particle profile on sentinel lymph node uptake2009 - NUNEZ, EUTIMIO G.F.; FAINTUCH, BLUMA L.; TEODORO, RODRIGO; WIECEK, DANIELLE P.; MARTINELLI, JOSE R.; SILVA, NATANAEL G. da; CASTANHEIRA, CLAUDIA E.; OLIVEIRA FILHO, RENATO S. de; PASQUALINI, ROBERTOArtigo IPEN-doc 17833 Radiolabeled nano-peptides show specificity for an animal model of human PC3 prostate cancer cells2011 - FAINTUCH, BLUMA L.; FERNANDEZ NUNEZ, GUSTAVO E.; TEODORO, RODRIGO; MORO, ANA M.; MENGATTI, JAIRArtigo IPEN-doc 18085 Combining dose and injection volume for good performance of a specific radiopharmaceutical for sentinel node detection2012 - NUNEZ, EUTIMIO G.F.; OLIVEIRA, ERICA A. de; SILVA, NATANAEL G. da; OLIVEIRA FILHO, RENATO S. de