ANDRE RICARDO MACHI
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Artigo IPEN-doc 26924 Ionizing radiation and the influence of package to control of Sitophilus oryzae in rice2017 - MACHI, ANDRE R.; HARDER, MARCIA N.C.; ARTHUR, PAULA B.; FRANCO, SUELY S.H.; ARTHUR, VALTERObjective: This study aimed to use the gamma radiation from Cobalt-60 to control of Sitophilus oryzae in package with previously infested rice and artificial reinfestation. Material and Methods: In the research, 3 types of sample were used: 2 commercial rice packages and 1 in crystal sugar packages. The samples were irradiated with a dose of 1.0kGy to promote disinfestations under a dose rate of 0.560 Gy/h. After 60 days were counted the number of live and dead insects in each package. Results: We concluded that the package containing holes for gases exchange from inside to outside of the package showed easily penetration of insects, while the two packages without holes prevented the reinfestation of insects. Conclusion: The dose of 1.0 kGy was sufficient to do the disinfestations of rice.Artigo IPEN-doc 26264 Disinfestation of mangoes haden infested by Anastrepha fraterculus (Wied., 1830) (Diptera, Tephritidae), with gamma radiation2019 - ARTHUR, PAULA B.; MACHI, ANDRE R.; LEANDRO, R.S.R.; ARTHUR, VALTERThe objective of experiment was determinate the radiation dose for disinfestation to mango Mangifera indica cv. Haden, infested by Anastrepha fraterculus larvae. For realization of the experiment, were collected fruits in the field, which were taking to Entomology laboratory where there was a infestation by the flies in cages during 72 hours period. Waited for the development of the larvae and before 5 – 8 days to infestation, the mangos were irradiated in a Cobalt-60 source with doses of: 0(control), 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1.100, 1.200 and 1.300 Gy. After the irradiation, the fruits were placed in climate chamber with 25 ±5°C of temperature and 70±5% of relative humidity, posteriorly waited the larvae exit to out of the fruit until the transformation in pupae and posteriorly in adult stage. By the results obtained our can concluded that the lethal dose to larvae in mangoes infested with 5-8 days after infestation were 600 Gy and 1.000 Gy (0.6 and 1 kGy) respectively. The dose of 50 Gy prevented the total adult emergence for both treatments.Artigo IPEN-doc 26262 Propagation of mango seedlings of the cultivar “Tommy” by grafts irradiated2019 - FRANCO, SUELY S.H.; FRANCO, JOSE G.; FERRARI, LUIZ; MACHI, ANDRE R.; LEANDRO, RODRIGO S.R.; ARTHUR, PAULA B.; ARTHUR, VALTERDue to difficulties of vegetative propagating of any pants, many tests were performed with ionizing radiation aiming the modification of the characteristics of the plants for obtaining of the improved genotypes and of smaller size. Rootstock “Espada” cultivar, were used to obtain mango seedlings of “Tommy” cultivar. The cuttings were irradiated with different doses of gamma radiation: 0 (control), 2.5, 5.0, 7.5 and 10.0 Gy, in a source of Cobalt-60 type Gammacell-220, installed in the Center for Nuclear Energy in Agriculture, CENA-USP. After irradiation, was performed the grafting type cleft graft. Was made the first evaluation of height of the plants from the budding grafting after 24 months. Data were subjected by statistic program (SAS) and the means were compared by Tukey test (p <0.05). From the results obtained can be concluded that the doses of 2.5 Gy stimulated growth of the plants and the lethal dose was 10.0 Gy because don’t have development of plants.Artigo IPEN-doc 26260 Sterilizing of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) irradiated in pupa stage2019 - ARTHUR, PAULA B.; VILLAVICENCIO, ANNA L.C.H.; MACHI, ANDRE R.; LEANDRO, RODRIGO S.R.; ARTHUR, VALTERWorldwide the loss of stored grain is a problem of economic order of importance, in view of the concern of the increased supply of food for a world population increasingly expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations in the resources of a country. This lack could be met by adequate supply of grain produced, requiring for it, a system that provides optimum grain storage conditions in securing the quality until the time of consumption. The use of radiation in stored grain can solve the problem of the losses in these products, as it does not induce resistance of insects and leaves no toxic residue to the consumer, and is considered an effective and safe method. The aim of the experiment was to determine the sterilizing dose of ionizing radiation from cobalt-60 to Alphitobius diaperinus (Panzer, 1797)(Coleoptera: Tenebrionidae) in peanuts irradiated in pupa stage. The experiment was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP., Piracicaba, SP, Brazil. Peanuts samples infested with pupae of A. diaperinus were utilized in the experiment that consisted of 8 treatments with 5 repetitions. Each repetition consisted of 20 pupae a total of 100 individuals per treatment. Were irradiated with doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a room with temperatures of 25 ± 5 ° C and relative humidity of 70 ± 5%. After of irradiation process was evaluated of the number of emerged adult insects in each repetition in the treatments. The results show that the sterilizing dose in F1 generation was 125 Gy and the pupa lethal dose 150 Gy. The dose of 150 Gy of gamma radiation can be used as phytosanitary treatment to control of immature stages of A. diaperinus infested peanuts.Artigo IPEN-doc 25778 Gamma radiation sterilization dose of adult males in Asian tiger mosquito pupae2019 - MACHI, ANDRE R.; MAYNE, RAQUEL R.; GAVA, MARCIO A.; ARTHUR, PAULA B.; ARTHUR, VALTERThe pathogen-carrying tiger mosquito, Aedes albopictus, has spread from theWestern Pacific and Southeast Asia to Europe, Africa, the Middle East, North and South America, and the Caribbean. This species of mosquito transmits arboviral infections, such as yellow fever, chikungunya, dengue, zika, and several encephalitides. The objective of this research was to provide a radiation dose inducing sterilization in adult male Ae. albopictus in the pupal stage. A cobalt-60 source of gamma radiation at a dose rate of 381 Gy/h was used. The pupae were irradiated with doses of 0 (control), 20, 30, 40, 50, and 60 Gy. Each treatment had a total of five replications using 60 pupae. After irradiation, the different phases of Ae. albopictus development (egg, larva, pupa, and adult) in the F1 generation were observed daily. Parameters such as viability, fertility, longevity, and mortality were recorded. The results from these studies showed that a dose of 60 Gy was necessary to sterilize 100% of the male Ae. albopictus pupae.Artigo IPEN-doc 25721 Efficacy of irradiated glyphosate against Brachiaria plantaginea L.2018 - MACHI, ANDRE R.; FERRARI, LUIZ S.; ARTHUR, VALTERGlyphosate irradiated with cobalt-60 type Gammacell 220 at different rates was sprayed on seedlings of Brachiaria plantaginea L., a common weed, grown in pots (one seedling with 3-4 leaves in each pot), at different concentrations equivalent to 1 L h-1 or 3 L h-1. Each sample containing 40 ml of the product (glyphosate) taken to a source of Cobalt-60 type Gammacell 220, under a dose rate of 0.312 kGy/hour in doses of: T0, control (water instead of glyphosate); T1, glyphosate at 1.0 L h-1; T2, glyphosate at 3.0 L h-1; T3, glyphosate irradiated at 250 Gy at 1.0 L h-1; T4, glyphosate irradiated at 500 Gy at 1.0 L h-1; T5, glyphosate irradiated at 750 Gy at 1.0 L h-1; and T6, glyphosate irradiated at 1000 Gy at 1.0 L h-1. The effects were assessed in terms of the extent of injury, where scale of 0% (no visible injury) to 100% (plant dead), number of tillers, weed survival and dry biomass produced by the plants. The most effective treatment was glyphosate irradiated at 250 Gy h-1 and applied at 1.0 L h-1. It is important to know that gamma radiation in glyphosate herbicide can be used without antagonistic interactions without cause resistance.Artigo IPEN-doc 25596 Control of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) by gamma radiation2018 - ARTHUR, PAULA B.; VILLAVICENCIO, ANNA L.; MACHI, ANDRÉ R.; ROSSI, RODRIGO S.; ARTHUR, VALTERThe aim of the experiment was to determine the sterilizing dose from ionizing radiation by cobalt-60 to Alphitobius diaperinus (Panzer, 1797)(Coleoptera: Tenebrionidae) in peanuts. The study was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP at Piracicaba, SP - Brazil. Peanuts samples infested with adults of A. diaperinus with 8 treatments and 5 repetitions were used. Each repetition consisted of 20 adults, a total of 100 individuals per treatment. The samples were irradiated in doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a room with a relative of 25 ± 5 ° C temperatures and humidity of 70 ± 5%. After 50 days of irradiation process was evaluated of the number of emerged insects in each treatment. The results show that the sterilizing doses in F1 and F2 generations respectively were: 150 Gy and 125 Gy. The dose of 150 Gy of gamma radiation can be used in both generations as phytosanitary treatment to control of A. diaperinus infested peanuts.Artigo IPEN-doc 24149 Quarantine treatment by gamma radiation for different development stages of Callosobruchus maculatus in bean Vigna sinensis2017 - ARTHUR, VALTER; FONTES, LUCIA da S.; ARTHUR, PAULA B.; MACHI, ANDRE R.; HARDER, MARCIA N.C.; ROSSI, RODRIGO S.; FRANCO, JOSE G.; FRANCO, SUELY S.H.The loss of stored grain caused by insects generates a problem of economic order of importance, due to concern about the increased supply of food for the world population is expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations. The use of ionizing radiation in grains and products stored without a doubt can solve the problem of the losses in these products, since it does not induce resistance to insects and leaves no toxic residue in the products, and is considered an effective and safe method. The aim of the experiment was to determine the effect of ionizing radiation from cobalt-60 as a quarantine treatment for the different stages of development of Callosobruchus maculatus (Fabr., 1972) (Coleóptera, Chysomilidae) in bean Vigna sinensis. The experiment was conducted in the laboratory of Radiobiology and Environment CENA / USP., Piracicaba, SP, Brazil. Bean samples infested with eggs, larvae, pre-pupae and pupae C. maculatus, the experiment consisted of 4 replicates for each stage of the insect's life cycle, and each repetition consisted of 20 individuals (eggs, larvae, pre-pupae and pupae), a total of 200 subjects per treatment which were irradiated with doses of 0 (control), 25, 50, 75 and 100 Gy, a source of cobalt-60, Gammabeam-650 type, in a rate dose of 1.3 kGy / h. The experiment was conducted in a room with a relative of 25 ± 2 ° C temperature and humidity of 70 ± 5%. After 35 days of irradiation process were carried out evaluations of the number of insects emerged in each repetition within the treatments. From the results obtained it was concluded that the dose lethal to eggs and larvae was 25 Gy, while for pre-pupae was 50 Gy, to pupae 100 Gy was not sufficient to control the adult emergence.Artigo IPEN-doc 24110 Gamma radiation for all phases of life cycle of cotton bollworm Helicoverpa armigera aiming at its control2017 - HADDAD, GIANNI Q.; MACHI, ANDRE R.; ARTHUR, VALTERSince the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (SIT), this insect control method traditionally uses ionizing radiations to sterilize insects, being a technique that does not generate residue, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within IPM programs, to overcome the resistance of chemical products, such as: reducing residues of pesticides. For some important crops of our country, we have a wide spectrum of pests occurring from beginning to end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars among them Helicoverpa armigera. Due to this the objective this study was establishes doses of gamma radiation to sterilizing of the phases of : eggs, larvae, pupae and adults of H. armigera aiming their control. The experiment was carried out with application of gamma radiation from a Cobalt-60 source. The treatments consisted of doses of gamma radiation varying of according with the insect phase, being this variation of: 0 (control) to 400 Gy. The experiments with pupae and adult phases showed satisfactory results in the sterilization of H. armigera for use in autocide control programs. The sterilize dose to adult and pupae phase were 400 Gy and 100 Gy respectively, being the best doses for the application of the sterile insect technique to this pest in cotton.Artigo IPEN-doc 23556 Milbemectin and etoxazol acaricide resistant and susceptible strains of Tetranychus urticae (Trombidiformes: Tetranychidae) are equally radiosusceptible and unable to reproduce when irradiated with 400 Gy2016 - ARTHUR, VALTER; NICASTRO, ROBERTO L.; SATO, MARIO E.; MACHI, ANDRE R.The twospotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is considered one of the most important phytophagous mites causing considerable damage in several agricultural crops. The aim of this study was to evaluate the susceptibility to gamma irradiation of strains of T. urticae resistant (R) and susceptible (S) to the acaricides, milbemectin and etoxazol. The R and S strains for milbemectin and etoxazol were irradiated with 200 and 400 Gy of gamma radiation in a Gammacell 220 source to evaluate the effects of gamma radiation on the growth rate of the mites. A dose of 400 Gy totally eliminated populations of both R and S strains of T. urticae within 10 d. A dose of 200 Gy was not sufficient to totally eliminate both T. urticae strains, but it significantly reduced egg viability of both strains. In the most likely measure of efficacy for phytosanitary irradiation of mites—i.e., prevention of F1 egg hatch when parent adults are irradiated—no differences were found in response to irradiation among the 4 strains.
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