KLEICY CAVALCANTE AMARAL
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Resumo IPEN-doc 29591 Cytototic activity and chemical profile of methanolic extract obtained from Avelós stem (Euphorbia tirucalli L.) Euphorbiaceae2022 - RUSSO, DANIELA C.; AMARAL, KLEICY C.; CALDAS, LHAIS A.; VIEIRA, DANIEL P.; SARTORELLI, PATRICIA; RIBEIRO FILHO, WALDEMAR A.Avelós (Euphorbia tirucalli Linnaeus), a plant selected for this study, has been popularly used in the fight against tumors, arousing the interest of researchers in this area so that it can be used safely in the auxiliary treatment of different types of cancer.1 It belongs to the family Euphorbiaceae and to the genus Euphorbia, it is also the object of studies related to the treatment of a range of infectious and inflammatory diseases. Herbaceous much used by popular and traditional medicine, presents, a latex rich in molecules that confirm its high toxicity. The objective of this research was to verify the chemical profile of the methanolic extract obtained from the stem (modified leaves) of the plant in question and to determine the cytotoxicity of the crude extract by cytotoxic assay against the lineages of adenocarcinoma (MCF-7) and neoplastic cells of human melanoma (SK-MEL-37).2 For this, the plant was collected, and after drying and milling the material was extracted with methanol. Subsequently, the present compounds were separated by the thin-layer chromatography technique and the classes of substances found in the extract were identified by the technique of Nuclear Magnetic Resonance of Hydrogen and Carbon-13 (NMR).3 The combination of cyclohexane with Acetone and Hexane (5:3:2) provided a suitable polarity for the elution of the extract, which was revealed with ultraviolet detection and different reagents: sulfuric acid solutions; aluminum chloride; ferric chloride; 10% potassium hydroxide in ethanol; green bromocresol indicator solution; potassium permanganate, Dragendorff Reagent, vanillin and iodine vapors. The phytochemical study of the methanolic extract of Euphorbia tirucalli allowed to identify the presence of phenolic compounds, flavonoids and terpenes, a result confirmed by NMR spectra. The cytotoxic potential assays, although they are in low concentration thus altering the result, show that the methanolic extract of Euphorbia tirucalli shows activity against the tested cell lines. The observed activity may be related, according to information available in the literature, with the classes identified in the samples studied.Resumo IPEN-doc 27469 Cytotoxic activity and chemical profile of methanolic extract obtained from Avelós stem (Euphorbia tirucalli L.) Euphorbiaceae2020 - RUSSO, DANIELA C.; AMARAL, KLEICY C.; CALDAS, LHAIS A.; VIEIRA, DANIEL P.; SARTORELLI, PATRICIA; RIBEIRO FILHO, WALDEMAR A.Avelós (Euphorbia tirucalli Linnaeus), a plant selected for this study, has been popularly used in the fight against tumors, arousing the interest of researchers in this area so that it can be used safely in the auxiliary treatment of different types of cancer.1 It belongs to the family Euphorbiaceae and to the genus Euphorbia, it is also the object of studies related to the treatment of a range of infectious and inflammatory diseases. Herbaceous much used by popular and traditional medicine, presents, a latex rich in molecules that confirm its high toxicity. The objective of this research was to verify the chemical profile of the metanolic extract obtained from the stem (modified leaves) of the plant in question and to determine the cytotoxicity of the crude extract by cytotoxic assay against the lineages of adenocarcinoma (MCF-7) and neoplastic cells of human melanoma (SK-MEL-37).2 For this, the plant was collected, and after drying and milling the material was extracted with methanol. Subsequently, the present compounds were separated by the thin-layer chromatography technique and the classes of substances found in the extract were identified by the technique of Nuclear Magnetic Resonance of Hydrogen and Carbon-13 (NMR).3 The combination of cyclohexane with Acetone and Hexane (5: 3: 2) provided a suitable polarity for the elution of the extract, which was revealed with ultraviolet detection and different reagents: sulfuric acid solutions; aluminum chloride; ferric chloride; 10% potassium hydroxide in ethanol; green bromocresol indicator solution; potassium permanganate, Dragendorff Reagent, vanillin and iodine vapors. The phytochemical study of the methanolic extract of Euphorbia tirucalli allowed to identify the presence of phenolic compounds, flavonoids and terpenes, a result that was confirmed by NMR spectra. The cytotoxic potential assays, although they are in low concentration thus altering the result, show that the methanolic extract of Euphorbia tirucalli shows activity against the tested cell lines. The observed activity may be related, according to information available in the literature, with the classes identified in the samples studied.Artigo IPEN-doc 26226 Effects of an inhibitor of nitric oxide production on cell cycle and micronucleus frequency in irradiated human breast adenocarcinoma cells2019 - AMARAL, KLEICY C.; CARVALHO, LUMA R. de; ALBIERO, ANA LIGIA; LAUBE, RAQUEL; NASCIMENTO, ANA C.G.; BONFIM, LETICIA; VIEIRA, DANIEL P.Breast adenocarcinomas ar e the most frequent malignant tumor (about 25% of cases), and its malignant outcome causes about 15% of all deaths of women with cancer. The production of nitric oxide (NO) by isoforms of nitric ox ide synthases (NOS’s) are related to increased malignancy a nd stimuli to metastatic progression of breast adenocarcinomas, but its presence in irradiated cells can lead to higher frequencies of DNA damage. The work used aminoguanidine, an inhibitor of isof orm 2 of NOS (NOS 2) to treat human breast cancer cells (MCF 7) in non toxic concentrations (1 and 2mM) before exposure to gamma irradiation 60 Co) to assess if reduction of intracellular NO can protect from, or induce radio induced cell damage, cell cycle disruption or death. Cells were treated and irradiated at 0, 0.5, 1, 2, 4 or 8Gy . A dministration of aminoguanidine arrest ed the cell cycle in the synthesis (S) phase, altering the DNA repair capacity of cells. T he higher concentration (2mM) led to less genotoxic damage (about 50%) in cells irradiated at 8Gy as obse rved using micronucleus scoring by flow cytometry. Alternatively, 1mM of aminoguanidin e increased genotoxic damage and induced a less significant increase of S phase cells. Despite the findings, no significant alterations in cell proliferation rates were o bserved. Finding s showed that aminogua ni dine can modulate radio induced ef fects on cells.