Semi quantitative evaluation of skin burn wound healing

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dc.contributor.authorCASTRO, PEDRO A.A. dept_BR
dc.contributor.authorZEZELL, DENISE M.pt_BR
dc.contributor.authorMORAIS, MYCHEL R.P.T.pt_BR
dc.contributor.authorZORN, TELMA M.pt_BR
dc.coverageNacionalpt_BR
dc.creator.eventoENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42.pt_BR
dc.date.accessioned2019-10-09T18:39:52Z
dc.date.available2019-10-09T18:39:52Z
dc.date.evento26-31 de maio, 2019pt_BR
dc.description.abstractThe role of skin wound healing the biochemical mechanisms are still not fully understood and spectroscopy may shine some light on this information. The Fourier Transform Infrared spectroscopy coupled with Attenuated Total Re°ectance (ATR-FTIR) has been demonstrated potential to monitor the biomolecule activity upon biological samples. This work aims to evaluate the feasibility of using ATR-FTIR to discriminate burned skin throughout wound stages. Water vapor at 90±C was applied to the dorsum of Wistar rats resulting in standardized third-degree burns. Tissue samples were collected after 3±, 7±, 14± and 21 days post burn injury. For the acquisition of the spectra, 150 scans were averaged with a resolution of 4 cm¡1 and wavenumbers ranging from 4000 to 400 cm¡1. Analysis of the spectra was calculated using MATLABR°R2015a (MathWorks, Natick, MA) software. Fingerprint region (900 - 1800 cm¡1) and high wavenumber (2800 - 3000 cm¡1) of spectra were o®set-corrected and vector normalized. Area under the curve (AUC) of collagen (1236 cm¡1), amide II (1540 cm¡1), amide I (1632 cm¡1) and lipid (2852 cm¡1) of each group were performed by integration method. The FTIR results exhibit the high biochemical activity of the tissue in the 14o post burn injury when it is compared with control group which correspond to the stage when the new tissue formation is still ongoing. The lipid content decrease in the 21 day, which suggest that the metabolic activity and structural reorganization decrease as the wound healing progress. For future works, micro-FTIR imaging will be used spatially discriminate the bands.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: CEPID 05/51689-2pt_BR
dc.description.sponsorshipIDCAPES: PROCAD 88881.068505/2014-01pt_BR
dc.description.sponsorshipIDCNPq: INCT 465763/2014-6; 141946/2018-0; PQ 309902/2017-7pt_BR
dc.event.siglaEOSBFpt_BR
dc.identifier.citationCASTRO, PEDRO A.A. de; ZEZELL, DENISE M.; MORAIS, MYCHEL R.P.T.; ZORN, TELMA M. Semi quantitative evaluation of skin burn wound healing: ATR-FTIR study. In: ENCONTRO DE OUTONO DA SOCIEDADE BRASILEIRA DE FÍSICA, 42., 26-31 de maio, 2019, Aracaju, SE. <b>Resumo...</b> São Paulo: Sociedade Brasileira de Física, 2019. Disponível em: http://repositorio.ipen.br/handle/123456789/30218.
dc.identifier.orcid0000-0001-7404-9606pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7404-9606
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30218
dc.localSão Paulopt_BR
dc.local.eventoAracaju, SEpt_BR
dc.publisherSociedade Brasileira de Físicapt_BR
dc.rightsopenAccesspt_BR
dc.titleSemi quantitative evaluation of skin burn wound healingpt_BR
dc.typeResumo de eventos científicospt_BR
dspace.entity.typePublication
ipen.autorDENISE MARIA ZEZELL
ipen.autorPEDRO ARTHUR AUGUSTO DE CASTRO
ipen.codigoautor693
ipen.codigoautor12053
ipen.contributor.ipenauthorDENISE MARIA ZEZELL
ipen.contributor.ipenauthorPEDRO ARTHUR AUGUSTO DE CASTRO
ipen.date.recebimento19-10
ipen.event.datapadronizada2019pt_BR
ipen.identifier.ipendoc26009pt_BR
ipen.notas.internasResumopt_BR
ipen.subtituloATR-FTIR studypt_BR
ipen.type.genreResumo
relation.isAuthorOfPublicationa565f8ad-3432-4891-98c0-a587f497db21
relation.isAuthorOfPublication4fc30bdc-40c6-4bd5-9431-96db978a0475
relation.isAuthorOfPublication.latestForDiscovery4fc30bdc-40c6-4bd5-9431-96db978a0475
sigepi.autor.atividadeZEZELL, DENISE M.:693:920:Npt_BR
sigepi.autor.atividadeCASTRO, PEDRO A.A. de:12053:920:Spt_BR
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