Vacuumed collagen-impregnated bioglass scaffolds

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dc.contributor.authorKIDO, HUELITON W.
dc.contributor.authorGABBAI-ARMELIN, PAULO R.
dc.contributor.authorAVANZI, INGRID R.
dc.contributor.authorSILVA, ANTONIO C. da
dc.contributor.authorFERNANDES, KELLY R.
dc.contributor.authorFORTULAN, CARLOS A.
dc.contributor.authorRENNO, ANA C.M.
dc.coverageInternacionalpt_BR
dc.date.accessioned2018-07-17T11:07:03Z
dc.date.available2018-07-17T11:07:03Z
dc.date.issued2019pt_BR
dc.description.abstractThis study evaluated physical–chemical characteristics of a vacuumed collagen-impregnated bioglass (BG) scaffolds and bone marrow stromal cells (BMSCs) behavior on those composites. scanning electron microscope and energy dispersive xray spectroscope demonstrated collagen (Col) was successfully introduced into BG. Vacuum impregnation system has showed efficiency for Col impregnation in BG scaffolds (approximately 20 wt %). Furthermore, mass weight decreasing and more stabilized pH were observed over time for BG/Col upon incubation in phosphate buffered saline compared to plain BG under same conditions. Calcium evaluation (Ca assay) demonstrated higher calcium uptake for BG/Col samples compared to BG. In addition, BG samples presented hydroxyapatite crystals formation on its surface after 14 days in simulated body fluid solution, and signs of initial degradation were observed for BG and BG/Col after 21 days. Fourier transform infrared spectroscopy spectra for both groups indicated peaks for hydroxyapatite formation. Finally, a significant increase of BMSCs viability for both composites was observed compared to control group, but no increase of osteogenic differentiation-related gene expressions were found. In summary, BG/Col scaffolds have improved degradation, pH equilibrium and Ca mineralization over time, accompanied by hydroxyapatite formation. Moreover, both BG and BG/Col scaffolds were biocompatible and noncytotoxic, promoting a higher cell viability compared to control. Future investigations should focus on additional molecular and in vivo studies in order to evaluate biomaterial performance for bone tissue engineering applications.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 14/23471-1pt_BR
dc.format.extent211-222pt_BR
dc.identifier.citationKIDO, HUELITON W.; GABBAI-ARMELIN, PAULO R.; AVANZI, INGRID R.; SILVA, ANTONIO C. da; FERNANDES, KELLY R.; FORTULAN, CARLOS A.; RENNO, ANA C.M. Vacuumed collagen-impregnated bioglass scaffolds: characterization and influence on proliferation and differentiation of bone marrow stromal cells. <b>Journal of Biomedical Materials Research Part B: applied biomaterials</b>, v. 107, n. 2, p. 211-222, 2019. DOI: <a href="https://dx.doi.org/10.1002/jbm.b.34112">10.1002/jbm.b.34112</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/28958.
dc.identifier.doi10.1002/jbm.b.34112pt_BR
dc.identifier.fasciculo2pt_BR
dc.identifier.issn1552-4973pt_BR
dc.identifier.percentilfi50.567en
dc.identifier.percentilfiCiteScore72.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/28958
dc.identifier.vol107pt_BR
dc.relation.ispartofJournal of Biomedical Materials Research Part B: applied biomaterialspt_BR
dc.rightsclosedAccesspt_BR
dc.subjectvacuum systems
dc.subjectmedium vacuum
dc.subjectimpregnation
dc.subjectcollagen
dc.subjectbone marrow cells
dc.subjectglass
dc.titleVacuumed collagen-impregnated bioglass scaffoldspt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorANTONIO CARLOS DA SILVA
ipen.codigoautor2949
ipen.contributor.ipenauthorANTONIO CARLOS DA SILVA
ipen.date.recebimento18-07pt_BR
ipen.identifier.fi2.831pt_BR
ipen.identifier.fiCiteScore5.3
ipen.identifier.ipendoc24743pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.subtitulocharacterization and influence on proliferation and differentiation of bone marrow stromal cellspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication839a3a2d-55ca-4376-993c-a8279c5cf017
relation.isAuthorOfPublication.latestForDiscovery839a3a2d-55ca-4376-993c-a8279c5cf017
sigepi.autor.atividadeSILVA, ANTONIO C. DA:2949:720:Npt_BR
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