Engineering of galectin-3 for glycan-binding optical imaging

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dc.contributor.authorDE LEO, THAIS C.pt_BR
dc.contributor.authorSANTOS, SOFIA N. dospt_BR
dc.contributor.authorANDRADE, CAMILLO D.C.pt_BR
dc.contributor.authorRICCI, EDUARDOpt_BR
dc.contributor.authorTURATO, WALTER M.pt_BR
dc.contributor.authorLOPES, NORBERTO P.pt_BR
dc.contributor.authorOLIVEIRA, RALPH S.pt_BR
dc.contributor.authorBERNARDES, EMERSON S.pt_BR
dc.contributor.authorDIAS-BARUFFI, MARCELOpt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-04-06T13:42:33Z
dc.date.available2020-04-06T13:42:33Z
dc.date.issued2020pt_BR
dc.description.abstractGalectin-3 (Gal-3) is a multifunctional glycan-binding protein that participates in many pathophysiological events and has been described as a biomarker and potential therapeutic target for severe disorders, such as cancer. Several probes for Gal-3 or its ligands have been developed, however both the pathophysiological mechanisms and potential biomedical applications of Gal-3 remain not fully assessed. Molecular imaging using bioluminescent probes provides great sensitivity for in vivo and in vitro analysis for both cellular and whole multicellular organism tracking and target detection. Here, we engineered a chimeric molecule consisting of Renilla luciferase fused with mouse Gal-3 (RLuc-mGal-3). RLuc-mGal-3 preparation was highly homogenous, soluble, active, and has molecular mass of 65,870.95 Da. This molecule was able to bind to MKN45 cell surface, property which was inhibited by the reduction of Gal-3 ligands on the cell surface by the overexpression of ST6GalNAc-I. In order to obtain an efficient and stable delivery system, RLuc-mGal-3 was adsorbed to poly-lactic acid nanoparticles, which increased binding to MKN45 cells in vitro. Furthermore, bioluminescence imaging showed that RLuc-mGal-3 was able to indicate the presence of implanted tumor in mice, event drastically inhibited by the presence of lactose. This novel bioluminescent chimeric molecule offers a safe and highly sensitive alternative to fluorescent and radiolabeled probes with potential application in biomedical research for a better understanding of the distribution and fate of Gal-3 and its ligands in vitro and in vivo.pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDCAPES: 88881.121165/2016-0pt_BR
dc.description.sponsorshipIDCNPq: 311947/2015-8pt_BR
dc.format.extent674-680pt_BR
dc.identifier.citationDE LEO, THAIS C.; SANTOS, SOFIA N. dos; ANDRADE, CAMILLO D.C.; RICCI, EDUARDO; TURATO, WALTER M.; LOPES, NORBERTO P.; OLIVEIRA, RALPH S.; BERNARDES, EMERSON S.; DIAS-BARUFFI, MARCELO. Engineering of galectin-3 for glycan-binding optical imaging. <b>Biochemical and Biophysical Research Communications</b>, v. 521, n. 3, p. 674-680, 2020. DOI: <a href="https://dx.doi.org/10.1016/j.bbrc.2019.10.161">10.1016/j.bbrc.2019.10.161</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31083.
dc.identifier.doi10.1016/j.bbrc.2019.10.161pt_BR
dc.identifier.fasciculo3pt_BR
dc.identifier.issn0006-291Xpt_BR
dc.identifier.orcid0000-0002-0029-7313pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0029-7313
dc.identifier.percentilfi50.18pt_BR
dc.identifier.percentilfiCiteScore62.50
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31083
dc.identifier.vol521pt_BR
dc.relation.ispartofBiochemical and Biophysical Research Communicationspt_BR
dc.rightsopenAccesspt_BR
dc.subjectproteins
dc.subjectcarbohydrates
dc.subjectbioluminescence
dc.subjectluciferase
dc.subjecttumor cells
dc.subjectlectins
dc.subjectmolecular biology
dc.subjectprobes
dc.subjectengineering
dc.subjectcorals
dc.titleEngineering of galectin-3 for glycan-binding optical imagingpt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorEMERSON SOARES BERNARDES
ipen.autorSOFIA NASCIMENTO DOS SANTOS
ipen.codigoautor12099
ipen.codigoautor14464
ipen.contributor.ipenauthorEMERSON SOARES BERNARDES
ipen.contributor.ipenauthorSOFIA NASCIMENTO DOS SANTOS
ipen.date.recebimento20-04
ipen.identifier.fi3.575pt_BR
ipen.identifier.fiCiteScore5.5
ipen.identifier.ipendoc26869pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublication8115c8bd-822c-4f5a-9f49-3c12570ed40a
relation.isAuthorOfPublicationab78881a-78eb-42be-a463-aaf80e70de3d
relation.isAuthorOfPublication.latestForDiscoveryab78881a-78eb-42be-a463-aaf80e70de3d
sigepi.autor.atividadeBERNARDES, EMERSON S.:12099:110:Npt_BR
sigepi.autor.atividadeSANTOS, SOFIA N. dos:14464:110:Npt_BR
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