Pancreas and liver uptake of new radiolabeled incretins (GLP-1 and Exendin-4) in models of diet-induced and diet-restricted obesity

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dc.contributor.authorSEO, DANIELE
dc.contributor.authorFAINTUCH, BLUMA L.
dc.contributor.authorOLIVEIRA, ERICA A. de
dc.contributor.authorFAINTUCH, JOEL
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-08-15T13:18:19Z
dc.date.available2017-08-15T13:18:19Z
dc.date.issued2017pt_BR
dc.description.abstractIntroduction: Radiolabeled GLP-1 and its analog Exendin-4, have been employed in diabetes and insulinoma. No protocol in conventional Diet-Induced Obesity (DIO), and Diet-Restricted Obesity (DRO), has been identified. Aiming to assess pancreatic beta cell uptake in DIO and DRO, a protocol was designed. Methods: GLP-1-βAla-HYNIC and HYNIC-βAla-Exendin-4 were labeled with technetium-99m. Four Swiss mouse models were adopted: Controls (C), Alloxan Diabetes Controls (ADC), DIO and DRO. Biodistribution and ex-vivo planar imaging were documented. Results: Radiolabeling yield was in the range of 97% and both agents were hydrophilic. Fasting Blood Glucose (FBG) was 79.2 ± 8.2 mg/dl in C, 590.4 ± 23.3 mg/dl in ADC, 234.3 ± 66.7 mg/dl in DIO, and 96.6 ± 9.3 in DRO (p = 0.010). Biodistribution confirmed predominantly urinary excretion. DIO mice exhibited depressed uptake in liver and pancreas, for both radiomarkers, in the range of ADC. DRO only partially restored such values. 99mTc-HYNIC-βAla-Exendin-4 demonstrated better results than GLP-1-βAla-HYNIC-99mTc. Conclusions: 1) Diet-induced obesity remarkably depressed beta cell uptake; 2) Restriction of obesity failed to normalize uptake, despite robust improvement of FBG; 3) HYNIC-βAla-Exendin-4 was the most useful marker; 4) Further studies are recommended in obesity and dieting, including bariatric surgery.pt_BR
dc.format.extent57-64pt_BR
dc.identifier.citationSEO, DANIELE; FAINTUCH, BLUMA L.; OLIVEIRA, ERICA A. de; FAINTUCH, JOEL. Pancreas and liver uptake of new radiolabeled incretins (GLP-1 and Exendin-4) in models of diet-induced and diet-restricted obesity. <b>Nuclear Medicine and Biology</b>, v. 49, p. 57-64, 2017. DOI: <a href="https://dx.doi.org/10.1016/j.nucmedbio.2017.03.002">10.1016/j.nucmedbio.2017.03.002</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27707.
dc.identifier.doi10.1016/j.nucmedbio.2017.03.002pt_BR
dc.identifier.issn0969-8051pt_BR
dc.identifier.percentilfi53.10en
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27707
dc.identifier.vol49pt_BR
dc.relation.ispartofNuclear Medicine and Biologypt_BR
dc.rightsopenAccesspt_BR
dc.subjectalloxan
dc.subjectbiomedical radiography
dc.subjectblood
dc.subjectdesign
dc.subjectdiet
dc.subjectexcretion
dc.subjectglucose
dc.subjectice
dc.subjectlabelling
dc.subjectliver
dc.subjectmetabolic diseases
dc.subjectmice
dc.subjectpancreas
dc.subjectsurgery
dc.subjecttechnetium
dc.subjecttechnetium 99
dc.subjectuptake
dc.subjectyields
dc.titlePancreas and liver uptake of new radiolabeled incretins (GLP-1 and Exendin-4) in models of diet-induced and diet-restricted obesitypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorBLUMA LINKOWSKI FAINTUCH
ipen.autorDANIELE SEO
ipen.codigoautor51
ipen.codigoautor6400
ipen.contributor.ipenauthorBLUMA LINKOWSKI FAINTUCH
ipen.contributor.ipenauthorDANIELE SEO
ipen.date.recebimento17-08pt_BR
ipen.identifier.fi2.203pt_BR
ipen.identifier.ipendoc23935pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreArtigo
relation.isAuthorOfPublicatione86aa86f-0601-4b44-aa7a-3ad168745a77
relation.isAuthorOfPublicationc96def2e-d2f1-4481-8209-ed17f2ffc2e6
relation.isAuthorOfPublication.latestForDiscoveryc96def2e-d2f1-4481-8209-ed17f2ffc2e6
sigepi.autor.atividadeSEO, DANIELE:6400:110:Spt_BR
sigepi.autor.atividadeFAINTUCH, BLUMA L.:51:110:Npt_BR
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