Photodynamic therapy associated with ionizing radiation in the treatment of triple-negative breast cancer cells
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2021
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Biophysical Reviews
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INTRODUCTION
Breast cancer is the most common cancer for women worldwide.
According to the World Health Organization, it is considered the 5 th
leading cause of death from cancer. Triple-negative breast cancer
(TNBC) is a subtype of this disease that represents around 20% of all
invasive breast cancer, whose main characteristics are resistance to conventional
treatments, such as exposure to ionizing radiation (IR). On the
order hand, the photodynamic therapy (PDT) using porphyrins and their
derivatives has been described in the literature as a potential therapy
against cancer.
OBJECTIVES
Thus, our goal in this work was to associate PDT and IR in the treatment
of TNBC.
MATERIALS AND METHODS
MDA-MB-231 cells at a concentration of 2x104 cells were submitted to
PDT using TMPyP porphyrin (30 μM) and a red light (660 ±11 nm) with
fluences of the 23 and 57.5 J/cm 2 (57.3 mW/cm 2). Immediately
post-PDT, cells were divided into groups: non-treated (control), only IR
and PDT associated with IR (PDT57+IR and PDT23+IR) and then, exposed
to IR with a dose of 2.5 Gy. Past 24-h of the PDT-session, the cell
viability, clonogenicity and total glutathione were verified.
DISCUSSION AND RESULTS
Cells exposed to IR not presented statistically significance difference
compared to the control group. However, treated groups showed around
38% lower cell viability in relation to the control and IR groups. For the
clonogenic assay a reduction of the approximately 65% was observed
between IR and treated groups. Regarding to the total glutathione, all
groups showed an increase when compared to control group.
Nonetheless, no were identified differences between IR and treated
groups.
CONCLUSION
Taken together, our results indicate that PDTassociate with IR may be an
ally in TNBC treatment.
Como referenciar
SILVA, CAMILA R.; PINTO, MAYARA S.; RIBEIRO, MARTHA S. Photodynamic therapy associated with ionizing radiation in the treatment of triple-negative breast cancer cells. Biophysical Reviews, v. 13, n. 6, p. 1386-1386, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32889. Acesso em: 21 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.