Expression of genes involved in porphyrin biosynthesis pathway in the human renal cell carcinoma
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2015
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Journal of Fluorescence
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Renal cell carcinoma (RCC) remains one of the
greatest challenges of urological oncology and is the third
leading cause of death in genitourinary cancers. Surgery
may be curative when patients present with localized disease.
Our previous results demonstrated the autofluorescence of
blood PpIX in primary RCC mouse model and an increase
in fluorescence intensity as a function of growth of the subcutaneous tumor mass. In another work, a nice correlation between the growth of the tumor mass and tissue fluorescence
intensity was found. The aim of this study was to evaluate the
expression profile of porphyrin biosynthesis pathway-related
genes of human kidney cells. We used two kidney cell lines,
one normal (HK2) and another malignant (Caki-1). Endogenous and 5-aminolevolinic acid (ALA) induced protoporphyrin IX (PpIX) HK2 and Caki-1 cells were analyzed by fluorescence spectroscopy. Real-time quantitative polymerase
chain reaction (qRT-PCR) was used to measure mRNA of
those genes. Emission spectra were obtained by exciting the
samples at 405 nm. For ALA untreated cells the maximum
fluorescence intensity was detected at 635 nm. The mean peak
area of emission spectra in both cells types increased linearly
in function of cell number. Besides, basal levels of PpIX autofluorescence of each cell concentration of HK2 samples
were significantly lower than those of Caki-1 samples. For
ALA-treated cells the mean PpIX spectra shows PpIX emission peak at 635 nm with a shoulder at 700 nm. Analysis of
PpIX fluorescence intensity ratio between tumor cells and
HK2 cells showed that fluorescence intensity was, on average,
26 times greater in tumor cells than in healthy cells. qRT-PCR
revealed that in Caki-1 ALA-treated cells, PEPT gene was
significantly up-regulated and FECH and HO-1 genes were
significantly down regulated in comparison with HK2 ALAtreated cells. In conclusion, our results demonstrate the preferential accumulation of ALA-induced PpIX in human RCC
and also indicate that PEPT1, FECH and HO-1 genes are
major contributors to this accumulation.
Como referenciar
ROCHA FILHO, HUGO N. da; SILVA, EVELIN C. da; SILVA, FLAVIA R.O.; COURROL, LILIA C.; MESQUITA, CARLOS H. de; BELLINI, MARIA H. Expression of genes involved in porphyrin biosynthesis pathway in the human renal cell carcinoma. Journal of Fluorescence, v. 25, p. 1363-1369, 2015. DOI: 10.1007/s10895-015-1626-x. Disponível em: http://repositorio.ipen.br/handle/123456789/25372. Acesso em: 21 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.