Application of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro study

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dc.contributor.authorLEAL, CINTIA
dc.contributor.authorALVARENGA, LETICIA
dc.contributor.authorSILVA, TAMIRES
dc.contributor.authorBUSSADORI, SANDRA
dc.contributor.authorKATO, ILKA
dc.contributor.authorRIBEIRO, MARTHA
dc.contributor.authorPRATES, RENATO
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-10-02T12:02:19Z
dc.date.available2017-10-02T12:02:19Z
dc.date.issued2017pt_BR
dc.description.abstractBackground: Streptococcus mutans is a microorganism associated with tooth decay; many genes that express adhesion, biofilm formation, extracellular polysaccharide, carbohydrate uptake and acid tolerance regulate its cariogenic properties. S. mutans inhabits a complex biofilm and it produces a large amount of exopolysaccharides to promote adhesion and enabling transport pumps. Photodynamic therapy involves the use of a photosensitizer (PS), which is absorbed by specific cells followed by irradiation with visible light, resulting in cell death. The aim of this study was to investigate antimicrobial photodynamic therapy on Streptococcus mutans in the presence of glucose. Study: Streptococcus mutans was grown in brain heart infusion (BHI) at 37 8C for 48 h. Inocula were prepared with pure colonies, which were suspended in phosphate buffered saline (PBS) with and without 50mM glucose. One-hundred micromolar methylene blue was used as photosensitizer and the experiments were performed with groups (control, irradiated with LED, FS without irradiation, and PDT 30, 60, and 120 s). Colony form units were counted and statistically analyzed (oneway ANOVA and Tukey 5%). Results: The irradiation as well as the photosensitizer in the dark did not cause cell death. In contrast, in experiments without glucose, PDT caused cell death proportional the amount of light used. The more light, the higher the inactivation of S. mutans and after 2 min a reduction of 7 orders of magnitude (100%) was observed. In experiments with glucose cell death was observed even increasing the radiant exposure. Conclusion: We concluded that PDT is a viable solution for inactivation of S. mutans in suspension, and that the presence of glucose activates efflux pumps in the bacterial cell wall, and it drastically reduces the effect of PDT.pt_BR
dc.format.extent47-47pt_BR
dc.identifier.citationLEAL, CINTIA; ALVARENGA, LETICIA; SILVA, TAMIRES; BUSSADORI, SANDRA; KATO, ILKA; RIBEIRO, MARTHA; PRATES, RENATO. Application of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro study. <b>Lasers in Surgery and Medicine</b>, v. 49, n. S28, p. 47-47, 2017. DOI: <a href="https://dx.doi.org/10.1002/lsm.22650">10.1002/lsm.22650</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27823.
dc.identifier.doi10.1002/lsm.22650pt_BR
dc.identifier.fasciculoS28pt_BR
dc.identifier.issn0196-8092pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27823
dc.identifier.vol49pt_BR
dc.relation.ispartofLasers in Surgery and Medicinept_BR
dc.rightsopenAccesspt_BR
dc.sourceAMERICAN SOCIETY FOR LASER MEDICINE AND SURGERY, 37thpt_BR
dc.subjectbacteria
dc.subjectcaries
dc.subjectsensitizers
dc.subjectphotosensitivity
dc.titleApplication of photodynamic therapy with led and methylene blue in streptococcus mutans in the presence of glucose- in vitro studypt_BR
dc.typeResumos em periódicospt_BR
dspace.entity.typePublication
ipen.autorRENATO ARAÚJO PRATES
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorILKA TIEMY KATO
ipen.autorTAMIRES GOMES DA SILVA
ipen.codigoautor4028
ipen.codigoautor574
ipen.codigoautor3089
ipen.codigoautor12061
ipen.contributor.ipenauthorRENATO ARAÚJO PRATES
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorILKA TIEMY KATO
ipen.contributor.ipenauthorTAMIRES GOMES DA SILVA
ipen.date.recebimento17-10pt_BR
ipen.identifier.fi2.312pt_BR
ipen.identifier.ipendoc23128pt_BR
ipen.range.fi1.500 - 2.999
ipen.type.genreResumo
relation.isAuthorOfPublicationfde20222-8ca2-4a9e-941b-516590cac130
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication6c5e2bd6-1ae0-41e8-a0f6-9b29d0c1b115
relation.isAuthorOfPublicationb7d2d3f1-c85c-4eda-8536-06393445fc97
relation.isAuthorOfPublication.latestForDiscoveryb7d2d3f1-c85c-4eda-8536-06393445fc97
sigepi.autor.atividadeSILVA, TAMIRES:12061:-1:Npt_BR
sigepi.autor.atividadeKATO, ILKA:3089:920:Npt_BR
sigepi.autor.atividadeRIBEIRO, MARTHA:574:920:Npt_BR
sigepi.autor.atividadePRATES, RENATO:4028:920:Npt_BR
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