Melanoma cell migration in response to red and near-infrared low-level light
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2021
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Biophysical Reviews
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INTRODUCTION
Cell migration plays an important role in tissue formation and cancer
progression. In vitro scratch assay has been used for many years to study
cell migration to mimic the migration of in vivo cells, and, thus, to evaluate
cancer growth. Low-level red and near-infrared light (LLL) can
increase normal cell migration. However, the impact of LLL on tumor
cells remains unclear.
OBJECTIVES
In this work, we aimed to evaluate the effects of a single LLL dose on
melanoma cell migration.
MATERIALS AND METHODS
B16F10 (murine melanoma) cells were cultivated in RPMI medium with
10% of fetal bovine serum until they reached 80% confluency. The cell
line was seeded in a 6-well plate at a density of 2x10 5 cells/well in
triplicate at two different moments. A wound scratch was performed to
disrupt the confluent cellmonolayerwith a 10 μL pipette tip. Immediately
after the injury, the cells were submitted to the LLL at two distinct wavelengths
(660 and 780 nm) provided by a LED and a laser, respectively,
delivering 3 different energies (1.3, 3.6, and 6 J) at an irradiance of 4.2
mW/cm 2 . The control group was not irradiated. Cells were
photographed immediately and at 3, 12, 24, and 36 h after the scratch.
The wound closure was measured using ImageJ software. To evaluate the
overall migration, we calculated the areas under the curve for each group.
DISCUSSION AND RESULTS
Cells exposed to the red laser at 6 J migrated slower than control. In
contrast, LLL at 780 nm promoted faster cell migration when irradiated
with 3.6 J.
CONCLUSION
These results suggest that low-level LEDs at 660 nm could prevent melanoma
progression in higher energies. However, 780 nm should be
avoided at middle energies.
Como referenciar
CONTATORI, CAROLINA G. de S.; PINTO, MAYARA S.; RIBEIRO, MARTHA S. Melanoma cell migration in response to red and near-infrared low-level light. Biophysical Reviews, v. 13, n. 6, p. 1380-1380, 2021. DOI: 10.1007/s12551-021-00845-2. Disponível em: http://repositorio.ipen.br/handle/123456789/32885. Acesso em: 19 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.