Radiochemical purity determination of technetium (99m)Tc-sestamibi by high performance liquid chromatography (HPLC)

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dc.contributor.authorMARTINS, PATRICIA de A.pt_BR
dc.contributor.authorFUKUMORI, NEUZA T.O.pt_BR
dc.contributor.authorSILVA, NATANAEL G. dapt_BR
dc.contributor.authorMATSUDA, MARGARETH M.N.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoINTERNATIONAL NUCLEAR ATLANTIC CONFERENCEpt_BR
dc.date.accessioned2020-01-07T13:50:26Z
dc.date.available2020-01-07T13:50:26Z
dc.date.eventoOctober 21-25, 2019pt_BR
dc.description.abstractThe assessment of the extent of reversible or irreversible mitochondrial damage after myocardial ischemia is performed by obtaining myocardial perfusion SPECT-CT images of Technetium-99m Sestamibi radiopharmaceutical (99mTc-2-methoxy-isobutyl-isonitrile; 99mTc-sestamibi; 99mTc-MIBI). For quality control purposes, the monograph of the United States Pharmacopoeia (USP) was followed. The determination of the radiochemical purity of 99mTc-sestamibi involves the use of two chromatographic methods: thin layer chromatography in reverse phase and high performance liquid chromatography. This work aims to determine the radiochemical purity of 99mTc-sestamibi by the HPLC method described in USP. The analyses were performed on a Shimadzu liquid chromatography, LC-20AT model, consisting of two pumps, degasser, automatic sample injector, UV-visible detector and Bioscan radioactivity detector. The column used was μBondapack C18 (3.9 x 300 mm, 10 μm, Waters) and the mobile phase was a mixture of acetonitrile, 0.05 mol L-1 ammonium sulfate and methanol (20:35:45). 5 μL of sample (approximately 250 μCi) was injected with a 2 mL min-1 mobile phase flow. According to the USP monograph, the retention time for 99mTc-sestamibi is 5-10 minutes and for the 99mTc-pentamibidimethylvinylisonitrile impurity is 6-13 minutes. Not less than 90% of the total radioactivity must be present as 99mTc-sestamibi and not more than 5% as 99mTc-pentamibidimethylvinylisonitrile. For 12 analyzed batches of MIBI-TEC® produced at IPEN/CNEN-SP, the product presented a retention time of 7 minutes and the 99mTc-pentamibidimethylvinylisonitrile impurity formation was not observed.pt_BR
dc.event.siglaINACpt_BR
dc.format.extent1855-1859pt_BR
dc.identifier.citationMARTINS, PATRICIA de A.; FUKUMORI, NEUZA T.O.; SILVA, NATANAEL G. da; MATSUDA, MARGARETH M.N. Radiochemical purity determination of technetium (99m)Tc-sestamibi by high performance liquid chromatography (HPLC). In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. <b>Proceedings...</b> Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 1855-1859. Disponível em: http://repositorio.ipen.br/handle/123456789/30588.
dc.identifier.orcid0000-0003-4455-3486pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4455-3486
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30588
dc.localRio de Janeiropt_BR
dc.local.eventoSantos, SPpt_BR
dc.publisherAssociação Brasileira de Energia Nuclear
dc.rightsopenAccesspt_BR
dc.subjecthigh-performance liquid chromatography
dc.subjectquality control
dc.subjectradiochemical analysis
dc.subjectsingle photon emission computed tomography
dc.subjecttechnetium 99
dc.titleRadiochemical purity determination of technetium (99m)Tc-sestamibi by high performance liquid chromatography (HPLC)pt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorMARGARETH MIE NAKAMURA MATSUDA
ipen.autorNATANAEL GOMES DA SILVA
ipen.autorNEUZA TAEKO OKASAKI FUKUMORI
ipen.autorPATRICIA DE ANDRADE MARTINS
ipen.codigoautor841
ipen.codigoautor750
ipen.codigoautor1514
ipen.codigoautor965
ipen.contributor.ipenauthorMARGARETH MIE NAKAMURA MATSUDA
ipen.contributor.ipenauthorNATANAEL GOMES DA SILVA
ipen.contributor.ipenauthorNEUZA TAEKO OKASAKI FUKUMORI
ipen.contributor.ipenauthorPATRICIA DE ANDRADE MARTINS
ipen.date.recebimento20-01
ipen.event.datapadronizada2019pt_BR
ipen.identifier.ipendoc26238pt_BR
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublicationfa01cabc-d76d-45ee-bb21-4994c14ba167
relation.isAuthorOfPublication7374d7a3-3ba1-4852-b93d-e578e851abd1
relation.isAuthorOfPublicationab0b8070-073b-490b-bc03-17888a1c4f1d
relation.isAuthorOfPublication0fd86237-824c-4605-9f62-9ede89aef62d
relation.isAuthorOfPublication.latestForDiscovery0fd86237-824c-4605-9f62-9ede89aef62d
sigepi.autor.atividadeMATSUDA, MARGARETH M.N.:841:110:Npt_BR
sigepi.autor.atividadeSILVA, NATANAEL G. da:750:110:Npt_BR
sigepi.autor.atividadeFUKUMORI, NEUZA T.O.:1514:120:Npt_BR
sigepi.autor.atividadeMARTINS, PATRICIA de A.:965:120:Spt_BR
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