Preclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescence

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dc.contributor.authorCABRAL, FERNANDA V.pt_BR
dc.contributor.authorSABINO, CAETANO P.pt_BR
dc.contributor.authorDIMMER, JESICA A.pt_BR
dc.contributor.authorSAUTER, ISMAEL P.pt_BR
dc.contributor.authorCORTEZ, MAURO J.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-06-19T19:37:56Z
dc.date.available2020-06-19T19:37:56Z
dc.date.issued2020pt_BR
dc.description.abstractCutaneous leishmaniasis (CL) is a neglected disease that promotes destructive lesions. Difficulties in treatment are related to accessibility of drugs, resistance and toxicity. Antimicrobial photodynamic therapy (APDT) has been emerging as a promising treatment for CL. In this work, we evaluated methylene blue (MB)-mediated APDT (MB-APDT) on Leishmania amazonensis in vitro and in vivo by bioluminescence technique. In vitro, MB-APDT was performed using a red LED (k = 660 11 nm, 100 mW cm 2) and MB (100 μM) at different light doses. In vivo, mice were infected and 4 weeks later, randomly divided into three groups: control, APDT 1 (single session) and APDT 2 (two sessions of MB-APDT). MB was used at 100 μM and energy dose was established at 150 J cm 2. Parasite burden, lesion size and pain were evaluated weekly for 4 weeks. In vitro, lethal dose for 90% parasite inactivation was achieved at 48.8 J cm 2. In vivo, although APDT 1 and APDT 2 groups have showed similar parasite burden after 4 weeks, two sessions were clinically better, especially considering the inflammatory process associated to CL. Our findings reinforce MB-APDT as a costeffective treatment to combat CL.pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDCNPq: 465763/2014-6pt_BR
dc.description.sponsorshipIDFAPESP: 12/24105-3pt_BR
dc.format.extent604-610pt_BR
dc.identifier.citationCABRAL, FERNANDA V.; SABINO, CAETANO P.; DIMMER, JESICA A.; SAUTER, ISMAEL P.; CORTEZ, MAURO J.; RIBEIRO, MARTHA S. Preclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescence. <b>Photochemistry and Photobiology</b>, v. 96, n. 3, p. 604-610, 2020. DOI: <a href="https://dx.doi.org/10.1111/php.13188">10.1111/php.13188</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31330.
dc.identifier.doi10.1111/php.13188pt_BR
dc.identifier.fasciculo3pt_BR
dc.identifier.issn0031-8655pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi48.47pt_BR
dc.identifier.percentilfiCiteScore73.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31330
dc.identifier.vol96pt_BR
dc.relation.ispartofPhotochemistry and Photobiologypt_BR
dc.rightsclosedAccesspt_BR
dc.subjectantimicrobial agents
dc.subjecttherapy
dc.subjectphotosensitivity
dc.subjectmethylene blue
dc.subjectparasites
dc.subjectbioluminescence
dc.subjectphotochemistry
dc.subjectin vivo
dc.subjectin vitro
dc.subjectmice
dc.titlePreclinical investigation of methylene blue-mediated antimicrobial photodynamic therapy on Leishmania parasites using real-time bioluminescencept_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorISMAEL PRETTO SAUTER
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorFERNANDA VIANA CABRAL
ipen.codigoautor14992
ipen.codigoautor574
ipen.codigoautor12732
ipen.contributor.ipenauthorISMAEL PRETTO SAUTER
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorFERNANDA VIANA CABRAL
ipen.date.recebimento20-06
ipen.identifier.fi3.421pt_BR
ipen.identifier.fiCiteScore5.3
ipen.identifier.ipendoc26678pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi3.000 - 4.499
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublication0cab68f4-20bc-4fd5-b062-46d25ab6e49a
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication622f5d85-e9c4-40d7-a55f-873e2a346f55
relation.isAuthorOfPublication.latestForDiscovery622f5d85-e9c4-40d7-a55f-873e2a346f55
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
sigepi.autor.atividadeSAUTER, ISMAEL P.:14992:920:Npt_BR
sigepi.autor.atividadeCABRAL, FERNANDA V.:12732:920:Spt_BR
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