Effects of near-infrared low level laser irradiation on melanoma cells

Página do item simplificado
dc.contributor.authorCONTATORI, CAROLINA G. de S.pt_BR
dc.contributor.authorSILVA, CAMILA R.pt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.contributor.editorCOSTA-FELIX, RODRIGOpt_BR
dc.contributor.editorMACHADO, JOÃO C.pt_BR
dc.contributor.editorALVARENGA, ANDRÉ V.pt_BR
dc.coverageInternacionalpt_BR
dc.creator.eventoBRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, 26thpt_BR
dc.date.accessioned2019-08-30T19:44:40Z
dc.date.available2019-08-30T19:44:40Z
dc.date.eventoOctober 21-25, 2018pt_BR
dc.description.abstractLow-level laser (LLL) therapy promotes biostimulating effects in cell cultures growing in nutritional deficit. However, the effects of LLLs on tumor cell lines remain controversial. Studies indicate stimulatory, inhibitory or even no influence in this type of cells. Therefore, the aim of this study was to evaluate the influence of LLL irradiation on the cell viability (with and without nutritional deficit) of human melanoma SKMEL 37 cells and murine melanoma B16F10 cells using an infrared laser (k = 780 nm) with different radiant exposures. The cell lines were subjected to the LLL 24 h after they were seeded in a 96-well plate at a density of 5 104 cells per well. The analysis of cell proliferation by mitochondrial activity occurred at intervals of 24 and 72 h after laser irradiation. At each time, culture medium was removed and 180 μL of PBS and 20 μL of MTT were added. The plates were incubated for 4 h and the absorbance was read in a microplate reader at 570 nm. Results showed a non-significant statistical difference among the groups for both cell lines regardless the nutritional medium. The metabolic pattern was similar among the groups. It is concluded that irradiation with 780 nm laser light at radiant exposures of 30, 90 and 150 J/cm2 and an output power of 40 mW does not promote cell proliferation on melanoma cell lines.pt_BR
dc.event.siglaCBEBpt_BR
dc.format.extent797-801pt_BR
dc.identifier.citationCONTATORI, CAROLINA G. de S.; SILVA, CAMILA R.; RIBEIRO, MARTHA S. Effects of near-infrared low level laser irradiation on melanoma cells. In: COSTA-FELIX, RODRIGO (ed.); MACHADO, JOÃO C. (ed.); ALVARENGA, ANDRÉ V. (ed.). In: BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, 26th, October 21-25, 2018, Armação de Buzios, RJ. <b>Proceedings...</b> Singapore: Springer Nature Singapore, 2019. p. 797-801. (IFMBE Proceedings, volume 70/2). DOI: <a href="https://dx.doi.org/10.1007/978-981-13-2517-5_122">10.1007/978-981-13-2517-5_122</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/30149.
dc.identifier.doi10.1007/978-981-13-2517-5_122pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30149
dc.localSingaporept_BR
dc.local.eventoArmação de Buzios, RJpt_BR
dc.publisherSpringer Nature Singaporept_BR
dc.relation.ispartofseriesIFMBE Proceedings, volume 70/2pt_BR
dc.rightsclosedAccesspt_BR
dc.subjectlasers
dc.subjecttherapy
dc.subjectirradiation
dc.subjectlaser radiation
dc.subjectmelanomas
dc.subjecttumor cells
dc.subjectradiation doses
dc.titleEffects of near-infrared low level laser irradiation on melanoma cellspt_BR
dc.typeTexto completo de eventopt_BR
dspace.entity.typePublication
ipen.autorCAMILA RAMOS SILVA
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorCAROLINA GOUVEA DE SOUZA CONTATORI
ipen.codigoautor11642
ipen.codigoautor574
ipen.codigoautor14379
ipen.contributor.ipenauthorCAMILA RAMOS SILVA
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorCAROLINA GOUVEA DE SOUZA CONTATORI
ipen.date.recebimento19-08
ipen.event.datapadronizada2019pt_BR
ipen.identifier.ipendoc25943pt_BR
ipen.identifier.ods3
ipen.notas.internasProceedingspt_BR
ipen.type.genreArtigo
relation.isAuthorOfPublication7b89c9af-427d-4f2a-bb3d-1f6252c548c2
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublicationf1eb22e7-7818-44f0-9752-af6d7f6e7d82
relation.isAuthorOfPublication.latestForDiscoveryf1eb22e7-7818-44f0-9752-af6d7f6e7d82
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
sigepi.autor.atividadeSILVA, CAMILA R.:11642:920:Npt_BR
sigepi.autor.atividadeCONTATORI, CAROLINA G. de S.:14379:920:Spt_BR
Pacote Original
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
25943.pdf
Tamanho:
269.55 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Baixar
Coleções
Eventos