Electrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfecta

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dc.contributor.authorGOMES, ALISSANDRA de M.pt_BR
dc.contributor.authorJESUS, GUSTAVO P.P. dept_BR
dc.contributor.authorZACARIAS, ENIO A.pt_BR
dc.contributor.authorYOSIDAKI, VANESSA L.pt_BR
dc.contributor.authorHIGUTI, ELIZApt_BR
dc.contributor.authorRANGEL, ERIKA B.pt_BR
dc.contributor.authorBARTOLINI, PAOLOpt_BR
dc.contributor.authorPERONI, CIBELE N.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2019-10-22T17:32:54Z
dc.date.available2019-10-22T17:32:54Z
dc.date.issued2019pt_BR
dc.description.abstractOsteogenesis imperfecta (OI) is an inherited connective tissue disease characterized by fragility, deformity and low bone density, as well as by other clinical manifestations. Type I OI is the mildest and most common form of the disease, caused by mutation in the COL1A1 gene, resulting in the production of only ~50% of normal collagen. The corresponding animal model is the oim mouse, presenting a phenotype very similar to human type I OI. We aim, for the first time, at evaluating the electrotransference of mouse growth hormone (mGH) gene, encoding a protein that already showed therapeutic effects, together with the administration of murine mesenchymal stem cells (MSCs), for improving heterozygous oim mice phenotype. We already prepared and evaluated two populations of MSCs (bone marrow and adipose tissue) that emit red fluorescence. These are administrated in different amounts and via three routes (intravenously, intraperitoneally or locally into the femoral condyles) using the in vivo imaging system and histological sections of femur, liver and kidneys, a methodology we recently set up and adapted to our specific conditions. Then, we will administer the most efficient MSCs population combined with mGH gene electrotransfer. In this bioassay, we are ready to analyse different parameters: body weight, total body, tail and femur length, bone mineral density and femur fragility by a biomechanical flexion test. The results will indicate if the administration of GH by gene therapy, together with MSCs infusion, can be a promising treatment for improving type I OI phenotype.pt_BR
dc.format.extent35-35pt_BR
dc.identifier.citationGOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA L.; HIGUTI, ELIZA; RANGEL, ERIKA B.; BARTOLINI, PAOLO; PERONI, CIBELE N. Electrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfecta. <b>Journal of Cell Science & Therapy</b>, v. 10, p. 35-35, 2019. DOI: <a href="https://dx.doi.org/10.4172/2157-7013-C2-053">10.4172/2157-7013-C2-053</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/30302.
dc.identifier.doi10.4172/2157-7013-C2-053pt_BR
dc.identifier.issn2157-7013pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8194-5230
dc.identifier.percentilfiSem Percentil
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30302
dc.identifier.vol10pt_BR
dc.relation.ispartofJournal of Cell Science & Therapypt_BR
dc.rightsopenAccesspt_BR
dc.sourceInternational Conference and Exhibition on Advanced Cell and Gene Therapy, 9th, March 21-22, 2019, Rome, Italypt_BR
dc.titleElectrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfectapt_BR
dc.typeResumos em periódicospt_BR
dspace.entity.typePublication
ipen.autorGUSTAVO PROTASIO PACHECO DE JESUS
ipen.autorVANESSA DE LUNA YOSIDAKI
ipen.autorCIBELE NUNES PERONI
ipen.autorPAOLO BARTOLINI
ipen.autorENIO APARECIDO ZACARIAS
ipen.autorALISSANDRA DE MOURA GOMES
ipen.codigoautor14229
ipen.codigoautor14922
ipen.codigoautor947
ipen.codigoautor1503
ipen.codigoautor14288
ipen.codigoautor14488
ipen.contributor.ipenauthorGUSTAVO PROTASIO PACHECO DE JESUS
ipen.contributor.ipenauthorVANESSA DE LUNA YOSIDAKI
ipen.contributor.ipenauthorCIBELE NUNES PERONI
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorENIO APARECIDO ZACARIAS
ipen.contributor.ipenauthorALISSANDRA DE MOURA GOMES
ipen.date.recebimento19-10
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc26093pt_BR
ipen.type.genreResumo
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relation.isAuthorOfPublicationd919a103-3e1b-4903-a7f9-9dee371ec875
relation.isAuthorOfPublication.latestForDiscoveryd919a103-3e1b-4903-a7f9-9dee371ec875
sigepi.autor.atividadePERONI, CIBELE N.:947:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:810:Npt_BR
sigepi.autor.atividadeYOSIDAKI, VANESSA L.:14922:810:Npt_BR
sigepi.autor.atividadeZACARIAS, ENIO A.:14288:810:Npt_BR
sigepi.autor.atividadeJESUS, GUSTAVO P.P. de:14229:810:Npt_BR
sigepi.autor.atividadeGOMES, ALISSANDRA de M.:14488:810:Spt_BR
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