Osteoconductive properties of two different bioactive glass forms (powder and fiber) combined with collagen
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2017
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Applied Surface Science
Resumo
Bioactive Glasses (BG) is a group of synthetic silica-based materials with the unique ability to bond to
living bone and can be used in bone repair. Although the osteogenic potential of BG, this material may
have not present sufficient osteoconductive and osteoinductive properties to allow bone regeneration,
especially in compromised situations. In order to overcome this limitation, it was proposed the combination
the BG in two forms (powder and fiber) combined with collagen type I (COL-1). The aim of this study
was to evaluate the BG/COL-based materials in terms of morphological characteristics, physicochemical
features and mineralization. Additionally, the second objective was to investigate and compare the
osteoconductive properties of two different bioactive glass forms (powder and fiber) enriched or not with
collagen using a tibial bone defect model in rats. For this, four different formulations (BG powder – BGp,
BG powder enriched with collagen – BGp/Col, BG fibers – BGf and BGp fibers enriched with collagen –
BGf/Col) were developed. The physicochemical and morphological modifications were analyzed by SEM,
FTIR, calcium assay and pH measurement. For in vivo evaluations, histopathology, morphometrical and
immunohistochemistry were performed in a tibial defect in rats. The FTIR analysis indicated that BGp and
BGf maintained the characteristic peaks for this class of material. Furthermore, the calcium assay showed
an increased Ca uptake in the BG fibers. The pH measurements revealed that BGp (with or without collagen)
presented higher pH values compared to BGf. In addition, the histological analysis demonstrated
no inflammation for all groups at the site of the injury, besides a faster material degradation and higher
bone ingrowth for groups with collagen. The immunohistochemistry analysis demonstrated Runx-2 and
Rank-L expression for all the groups. Those findings support that BGp with collagen can be a promising
alternative for treating fracture of difficult consolidation.
Como referenciar
MAGRI, ANGELA M.P.; FERNANDES, KELLY R.; UENO, FABIO R.; KIDO, HUELITON W.; SILVA, ANTONIO C. da; BRAGA, FRANCISCO J.C.; GRANITO, RENATA N.; GABBAI-ARMELIN, PAULO R.; RENNO, ANA C.M. Osteoconductive properties of two different bioactive glass forms (powder and fiber) combined with collagen. Applied Surface Science, v. 423, p. 557-565, 2017. DOI: 10.1016/j.apsusc.2017.06.152. Disponível em: http://repositorio.ipen.br/handle/123456789/27749. Acesso em: 10 May 2024.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.