Biological activity of different batches of equine chorionic gonadotropin as determined by reversed-phase high-performance liquid chromatography and in vivo assay

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dc.contributor.authorALVAREZ, RAFAEL H.
dc.contributor.authorNATAL, FABIO L.N.
dc.contributor.authorALMEIDA, BEATRIZ E.
dc.contributor.authorOLIVEIRA, JOAO E.
dc.contributor.authorMELO, ALFREDO J.F.
dc.contributor.authorRIBELA, MARIA T.C.
dc.contributor.authorBARTOLINI, PAOLO
dc.coverageInternacionalpt_BR
dc.date.accessioned2017-10-05T18:57:32Z
dc.date.available2017-10-05T18:57:32Z
dc.date.issued2017pt_BR
dc.description.abstractAims: To evaluate the physicochemical profile of commercial batches of eCG, in order to find if differences can be related to their biological activity. Study Design: Commercial eCG was analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC) and in vivo bioassay. Place and Duration of Study: Department of Biotechnology (IPEN-CNEN) and Animal antibody production Laboratory (Animal Science Institute), between June 2013 and April 2014. Methodology: Two commercial eCG batches for veterinary use (I and II) and an eCG official International Standard from the World Health Organization (WHO) were analyzed by RP-HPLC. Additionally, two experiments were designed to validate the biological activity. In experiment 1, groups of prepubertal 21–25 day old Wistar female rats (n = 6/group) received the equivalent to 0 UI (saline) and 10 IU of eCG of each one of these preparations. Autopsy was performed 48 h later and ovaries were removed and weighed. The experiment 2 was designed to determine whether increasing the dose of less active eCG batches could increase the ovarian response. Therefore, groups of prepubertal rats (n = 6/group) were treated with 10 and 30 IU eCG from batch II, while eCG from WHO (10 IU) and saline were control. The evaluation of ovarian response was done similar to Experiment 1. Differences among treatments were analyzed by one-way ANOVA. Results: Results of RP-HPLC showed differences in the main tR peak profile (tR 26.7) of the standard WHO compared with eCG batches I and II. In experiment 1, the average ovarian weight of rats treated with eCG from WHO (60.0 ± 12.1 mg) was higher (P < .01) than saline (23.1 ± 1.6 mg) and batches I (37.6 ± 1.4 mg) and II (31.0 ± 4.3 mg). In experiment 2, the ovarian weight of rats treated with 30 IU of eCG of batch II (45.7 ± 4.1 mg) was higher (P < .01) than saline (32.6 ± 1.4 mg) and significantly lower (P = .05) than 10 UI of the standard WHO (63.3 ± 8.1 mg). Conclusion: The low ovarian response to eCG treatments can be related to differences in the physicochemical profile of eCG batches and RP-HPLC is a fast and reliable tool for detecting these differences.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipIDFAPESP: 11/13096-0pt_BR
dc.format.extent1-9pt_BR
dc.identifier.citationALVAREZ, RAFAEL H.; NATAL, FABIO L.N.; ALMEIDA, BEATRIZ E.; OLIVEIRA, JOAO E.; MELO, ALFREDO J.F.; RIBELA, MARIA T.C.; BARTOLINI, PAOLO. Biological activity of different batches of equine chorionic gonadotropin as determined by reversed-phase high-performance liquid chromatography and in vivo assay. <b>Journal of Advances in Medical and Pharmaceutical Sciences</b>, v. 12, n. 2, p. 1-9, 2017. DOI: <a href="https://dx.doi.org/10.9734/JAMPS/2017/31956">10.9734/JAMPS/2017/31956</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/27850.
dc.identifier.doi10.9734/JAMPS/2017/31956pt_BR
dc.identifier.fasciculo2pt_BR
dc.identifier.issn2394-1111pt_BR
dc.identifier.percentilfiSem Percentilen
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/27850
dc.identifier.vol12pt_BR
dc.relation.ispartofJournal of Advances in Medical and Pharmaceutical Sciencespt_BR
dc.rightsopenAccesspt_BR
dc.subjectliquid column chromatography
dc.subjectantibody formation
dc.subjectelectrocardiograms
dc.subjecthigh-performance liquid chromatography
dc.subjectbioassay
dc.subjectgonadotropins
dc.titleBiological activity of different batches of equine chorionic gonadotropin as determined by reversed-phase high-performance liquid chromatography and in vivo assaypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorPAOLO BARTOLINI
ipen.autorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.autorJOAO EZEQUIEL DE OLIVEIRA
ipen.autorBEATRIZ ELANE DE ALMEIDA
ipen.codigoautor1503
ipen.codigoautor1197
ipen.codigoautor425
ipen.codigoautor6043
ipen.contributor.ipenauthorPAOLO BARTOLINI
ipen.contributor.ipenauthorMARIA TERESA DE CARVALHO PINTO RIBELA
ipen.contributor.ipenauthorJOAO EZEQUIEL DE OLIVEIRA
ipen.contributor.ipenauthorBEATRIZ ELANE DE ALMEIDA
ipen.date.recebimento17-10pt_BR
ipen.identifier.fiSem F.I.pt_BR
ipen.identifier.ipendoc23146pt_BR
ipen.type.genreArtigo
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relation.isAuthorOfPublication2c1f5cfd-da46-4b49-b209-77aadfb388f1
relation.isAuthorOfPublicatione9e6346c-9b8b-4d39-8bdd-505ea6997646
relation.isAuthorOfPublication30050097-a62f-4d89-b572-899cdc6f3b93
relation.isAuthorOfPublication.latestForDiscovery30050097-a62f-4d89-b572-899cdc6f3b93
sigepi.autor.atividadeALMEIDA, BEATRIZ E.:6043:-1:Npt_BR
sigepi.autor.atividadeOLIVEIRA, JOAO E.:425:810:Npt_BR
sigepi.autor.atividadeRIBELA, MARIA T.C.:1197:810:Npt_BR
sigepi.autor.atividadeBARTOLINI, PAOLO:1503:810:Npt_BR
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