Effects of low-level laser irradiation on VEGF expression of melanoma cell lines

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dc.contributor.authorCONTATORI, C.G. de S.pt_BR
dc.contributor.authorSILVA, C.R.pt_BR
dc.contributor.authorYOSHIMURA, T.M.pt_BR
dc.contributor.authorRIBEIRO, M.S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-04-07T17:30:27Z
dc.date.available2020-04-07T17:30:27Z
dc.date.issued2020pt_BR
dc.description.abstractImpact of low-level laser irradiation on tumor cell lines remains controversial. Vascular endothelial growth factor (VEGF) is a key molecule to form new blood vessels, which contribute for cancer development and growth. The aim of this study was to evaluate the effects of different light fluences on human melanoma SKMEL 37 cells and murine melanoma B16F10 cells using a near infrared laser (λ= 780 nm) with output power of 40 mW delivering energies of 1.2, 3.6 and 6 J (fluences of 30, 90 and 150 J/cm2, respectively). The cell lines were irradiated 24 h after they were seeded in a 96-well plate at a density of 5x103 cells per well, in triplicate at three different days. Following irradiation, both cell line supernatants were stored in Eppendorf tubes at - 20°C until VEGF-A expression measurement. Specific ELISA kits were used according to cell line (murine or human). Samples and standard solutions were added in a 96-well plate antibody-coated and incuba ted over night at 4°C. Reagent dilution and set time followed fabricant instructions. The stop solution was added and the absorbance was read in a microplate reader at 450 nm. Results showed a non-statistically significant difference among treated and control groups for both cell lines. These findings indicate that irradiation with near infrared laser does not influence VEGF expression on melanoma cell lines regardless the fluence used and should be tested to prevent cancer growth in preclinical assays.pt_BR
dc.format.extent269-269pt_BR
dc.identifier.citationCONTATORI, C.G. de S.; SILVA, C.R.; YOSHIMURA, T.M.; RIBEIRO, M.S. Effects of low-level laser irradiation on VEGF expression of melanoma cell lines. <b>Lasers in Medical Science</b>, v. 35, n. 1, p. 269-269, 2020. DOI: <a href="https://dx.doi.org/10.1007/s10103-019-02900-7">10.1007/s10103-019-02900-7</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31110.
dc.identifier.doi10.1007/s10103-019-02900-7pt_BR
dc.identifier.fasciculo1pt_BR
dc.identifier.issn0268-8921pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi56.351pt_BR
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31110
dc.identifier.vol35pt_BR
dc.relation.ispartofLasers in Medical Sciencept_BR
dc.rightsopenAccesspt_BR
dc.sourceInternational Congress on Laser Medicine and Surgery, 30th, November 7-9, 2019, Florence, Italypt_BR
dc.subjectlaser radiation
dc.subjectlow dose irradiation
dc.subjectdoses
dc.subjectirradiation
dc.subjecttumor cells
dc.subjectmelanomas
dc.subjectenzyme immunoassay
dc.titleEffects of low-level laser irradiation on VEGF expression of melanoma cell linespt_BR
dc.typeResumos em periódicospt_BR
dspace.entity.typePublication
ipen.autorCAMILA RAMOS SILVA
ipen.autorMARTHA SIMOES RIBEIRO
ipen.autorTANIA MATEUS YOSHIMURA
ipen.autorCAROLINA GOUVEA DE SOUZA CONTATORI
ipen.codigoautor11642
ipen.codigoautor574
ipen.codigoautor10230
ipen.codigoautor14379
ipen.contributor.ipenauthorCAMILA RAMOS SILVA
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.contributor.ipenauthorTANIA MATEUS YOSHIMURA
ipen.contributor.ipenauthorCAROLINA GOUVEA DE SOUZA CONTATORI
ipen.date.recebimento20-04
ipen.identifier.fi2.342pt_BR
ipen.identifier.ipendoc26888pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi50.00 - 74.99
ipen.type.genreResumo
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relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication36384aec-6adf-42ef-99ae-68f62b9a69b9
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relation.isAuthorOfPublication.latestForDiscoveryf1eb22e7-7818-44f0-9752-af6d7f6e7d82
sigepi.autor.atividadeRIBEIRO, M.S.:574:920:Npt_BR
sigepi.autor.atividadeYOSHIMURA, T.M.:10230:920:Npt_BR
sigepi.autor.atividadeSILVA, C.R.:11642:920:Npt_BR
sigepi.autor.atividadeCONTATORI, C.G. de S.:14379:920:Spt_BR
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