Phenotype changes of oral epithelial stem cells after in vitro culture

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dc.contributor.authorDALTOE, FELIPE P.pt_BR
dc.contributor.authorOLIVEIRA, NÉLIO A.J. dept_BR
dc.contributor.authorPERONI, CIBELE N.pt_BR
dc.contributor.authorSHARPE, PAUL T.pt_BR
dc.contributor.authorMANTESSO, ANDREApt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2020-07-22T18:46:24Z
dc.date.available2020-07-22T18:46:24Z
dc.date.issued2020pt_BR
dc.description.abstractThe aim of our study was to isolate populations of keratinocyte stem cells based on the expression of cell surface markers and to investigate whether the culture could affect their phenotype. keratinocytes from human oral mucosa were sorted based on the expression of the epithelial stem cell markers p75NTR and CD71. We also examined the co-expression of other epithelial stem markers such as integrins β1 and α6 and their stem cell-like proprieties in in vitro assays. Three passages after being sorted by MACS, more than 93% of the p75NTR+ve cells lost the expression of p75NTR, while 5.46% of the p75NTR-ve gained it. Within the small population of the p75NTR+ve cells, 88% co-expressed other epithelial stem cell markers such as integrins β1 and α6, while only 28% of p75NTR-ve cells co-expressed these markers. These results were confirmed by sorting cells by FACS. Additionally, when double staining was used for sorting cells, 99% of the p75NTR+veCD71-ve and 33% of the p75NTR-veCD71+ve cells expressed both integrins, but just one week after culture, only 1.74% of the p75NTR+veCD71-ve cells still expressed p75NTR and only 0.32% still expressed CD71. Similar results were obtained when co-culturing p75NTR+ve and p75NTR-ve populations before analysis. Our results suggest that phenotype changes may be part of an intrinsic cellular mechanism to conserve levels of protein expression as they may found in the human body. In addition, in vitro culture may not offer ideal conditions for epithelial stem cell maintenance due to phenotype changes under standard culture conditions.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorshipIDFAPESP: 08/11641-9pt_BR
dc.description.sponsorshipIDCAPES: 2586-11-8pt_BR
dc.format.extent1-8pt_BR
dc.identifier.citationDALTOE, FELIPE P.; OLIVEIRA, NÉLIO A.J. de; PERONI, CIBELE N.; SHARPE, PAUL T.; MANTESSO, ANDREA. Phenotype changes of oral epithelial stem cells after in vitro culture. <b>Brazilian Oral Research</b>, v. 34, p. 1-8, 2020. DOI: <a href="https://dx.doi.org/10.1590/1807-3107bor-2020.vol34.0033">10.1590/1807-3107bor-2020.vol34.0033</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/31352.
dc.identifier.doi10.1590/1807-3107bor-2020.vol34.0033pt_BR
dc.identifier.issn1806-8324pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8194-5230
dc.identifier.percentilfi41.85pt_BR
dc.identifier.percentilfiCiteScore72.00
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/31352
dc.identifier.vol34pt_BR
dc.relation.ispartofBrazilian Oral Researchpt_BR
dc.rightsopenAccesspt_BR
dc.subjectstem cells
dc.subjectphenotype
dc.subjectmucous membranes
dc.subjectoral cavity
dc.subjectepithelium
dc.subjectanimal cells
dc.subjectanimal tissues
dc.subjectcell cultures
dc.subjectin vitro
dc.titlePhenotype changes of oral epithelial stem cells after in vitro culturept_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorCIBELE NUNES PERONI
ipen.autorNÉLIO ALESSANDRO DE JESUS OLIVEIRA
ipen.codigoautor947
ipen.codigoautor6042
ipen.contributor.ipenauthorCIBELE NUNES PERONI
ipen.contributor.ipenauthorNÉLIO ALESSANDRO DE JESUS OLIVEIRA
ipen.date.recebimento20-07
ipen.identifier.fi2.303pt_BR
ipen.identifier.fiCiteScore3.4
ipen.identifier.ipendoc27126pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublicationc30bdc49-9059-4fa3-91a9-7c92d7cd1dab
relation.isAuthorOfPublication85a21d3e-d0c8-434e-ab44-2e95b3019811
relation.isAuthorOfPublication.latestForDiscovery85a21d3e-d0c8-434e-ab44-2e95b3019811
sigepi.autor.atividadePERONI, CIBELE N.:947:810:Npt_BR
sigepi.autor.atividadeOLIVEIRA, NÉLIO A.J. de:6042:810:Npt_BR
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