Inactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapy

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dc.contributor.authorSABINO, CAETANO P.pt_BR
dc.contributor.authorWAINWRIGHT, MARKpt_BR
dc.contributor.authorANJOS, CAROLINA dospt_BR
dc.contributor.authorSELLERA, FABIO P.pt_BR
dc.contributor.authorBAPTISTA, MAURICIO S.pt_BR
dc.contributor.authorLINCOPAN, NILTONpt_BR
dc.contributor.authorRIBEIRO, MARTHA S.pt_BR
dc.coverageInternacionalpt_BR
dc.date.accessioned2019-12-12T17:06:48Z
dc.date.available2019-12-12T17:06:48Z
dc.date.issued2019pt_BR
dc.description.abstractBackground: Antimicrobial Photodynamic therapy (A-PDT) has been used to treat infections. Currently, microbial inactivation data is reported presenting survival fraction averages and standard errors as discrete points instead of a continuous curve of inactivation kinetics. Standardization of this approach would allow clinical protocols to be introduced globally, instead of the piecemeal situation which currently applies. Methods: To this end, we used a power-law function to fit inactivation kinetics and directly report values of lethal doses (LD) and a tolerance factor (T) that informs if inactivation rate varies along the irradiation procedure. A deduced formula was also tested to predict LD for any given survival fraction value. We analyzed the photoantimicrobial effect caused by red light activation of methylene blue (MB-APDT) and by blue light (BL) activation of endogenous microbial pigments against 5 clinically relevant pathogens. Results: Following MB- APDT, Escherichia coli and Staphylococcus aureus cells become increasingly more tolerant to inactivation along the irradiation process (T < 1). Klebsiella pneumoniae presents opposite behavior, i.e., more inactivation is observed towards the end of the process (T > 1). P. aeruginosa and Candida albicans present constant inactivation rate (T˜1). In contrast, all bacterial species presented similar behavior during inactivation caused by BL, i.e., continuously becoming more sensitive to blue light exposure (T > 1). Conclusion: The power-law function successfully fit all experimental data. Our proposed method precisely predicted LD and T values. We expect that these analytical models may contribute to more standardized methods for comparisons of photodynamic inactivation efficiencies.pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipIDFAPESP: 13/07937-8; 16/08593-9; 16/25095-2; 17/22406-0pt_BR
dc.description.sponsorshipIDCNPq: 465763/2014-6; 141901/2016-0; 312249/2017-9pt_BR
dc.format.extent186-191pt_BR
dc.identifier.citationSABINO, CAETANO P.; WAINWRIGHT, MARK; ANJOS, CAROLINA dos; SELLERA, FABIO P.; BAPTISTA, MAURICIO S.; LINCOPAN, NILTON; RIBEIRO, MARTHA S. Inactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapy. <b>Photodiagnosis and Photodynamic Therapy</b>, v. 28, p. 186-191, 2019. DOI: <a href="https://dx.doi.org/10.1016/j.pdpdt.2019.08.022">10.1016/j.pdpdt.2019.08.022</a>. Disponível em: http://repositorio.ipen.br/handle/123456789/30437.
dc.identifier.doi10.1016/j.pdpdt.2019.08.022pt_BR
dc.identifier.issn1572-1000pt_BR
dc.identifier.orcid0000-0002-4203-1134pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4203-1134
dc.identifier.percentilfi41.189pt_BR
dc.identifier.percentilfiCiteScore63.25
dc.identifier.urihttp://repositorio.ipen.br/handle/123456789/30437
dc.identifier.vol28pt_BR
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapypt_BR
dc.rightsopenAccesspt_BR
dc.subjectantimicrobial agents
dc.subjecttherapy
dc.subjectchemotherapy
dc.subjectinactivation
dc.subjectlethal doses
dc.subjectlight sources
dc.subjectdata analysis
dc.subjectcandida
dc.subjectmethylene blue
dc.titleInactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapypt_BR
dc.typeArtigo de periódicopt_BR
dspace.entity.typePublication
ipen.autorMARTHA SIMOES RIBEIRO
ipen.codigoautor574
ipen.contributor.ipenauthorMARTHA SIMOES RIBEIRO
ipen.date.recebimento19-12
ipen.identifier.fi2.894pt_BR
ipen.identifier.fiCiteScore4.1
ipen.identifier.ipendoc26453pt_BR
ipen.identifier.iwosWoSpt_BR
ipen.identifier.ods3
ipen.range.fi1.500 - 2.999
ipen.range.percentilfi25.00 - 49.99
ipen.type.genreArtigo
relation.isAuthorOfPublication36215a53-0150-4910-91d7-9559717b62d7
relation.isAuthorOfPublication.latestForDiscovery36215a53-0150-4910-91d7-9559717b62d7
sigepi.autor.atividadeRIBEIRO, MARTHA S.:574:920:Npt_BR
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